GENETIC-ANALYSIS OF THE RHIZOBIUM-MELILOTI EXOYFQ OPERON - EXOY IS HOMOLOGOUS TO SUGAR TRANSFERASES AND EXOQ REPRESENTS A TRANSMEMBRANE PROTEIN

MULLER P, KELLER M, WENG WM, QUANDT J, Arnold W, Pühler A (1993)
MOLECULAR PLANT-MICROBE INTERACTIONS 6(1): 55-65.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
; ; ; ; ;
Abstract / Bemerkung
The nucleotide sequence of a 4.8-kb ClaI-EcoRI DNA fragment of megaplasmid 2 of Rhizobium meliloti Rm2011 involved in succinoglucan (EPS I) synthesis and nodule infection was determined. Four open reading frames (ORFs) were identified on this fragment. A mutational analysis revealed that these ORFs represent genes that were termed exoX, exoY, exoF, and exoQ. The locations of transposon insertions in these exo genes were determined at the nucleotide level. Plasmid integration mutagenesis revealed that the genes exoY, exoF, and exoQ are organized in an operon. The exoX gene running in opposite direction forms a monocistronic transcriptional unit. The exoX gene was shown to negatively influence the amount of EPS I synthesized. The exoY gene is coding for a membrane associated protein homologous to the C-terminal part of the Xanthomonas campestris glucosyltransferase GumD and the Salmonella typhimurium galactose transferase RfbP. ExoF, a probable periplasmatic protein, is nearly identical to the protein encoded by ORF1 of Rhizobium sp. strain NGR234. ExoQ is most probably a membrane associated protein as deduced by its hydrophobic structural features. All three genes of the exoYFQ operon were shown to be essential for succinoglucan synthesis and nodule infection.
Erscheinungsjahr
Zeitschriftentitel
MOLECULAR PLANT-MICROBE INTERACTIONS
Band
6
Zeitschriftennummer
1
Seite
55-65
ISSN
PUB-ID

Zitieren

MULLER P, KELLER M, WENG WM, QUANDT J, Arnold W, Pühler A. GENETIC-ANALYSIS OF THE RHIZOBIUM-MELILOTI EXOYFQ OPERON - EXOY IS HOMOLOGOUS TO SUGAR TRANSFERASES AND EXOQ REPRESENTS A TRANSMEMBRANE PROTEIN. MOLECULAR PLANT-MICROBE INTERACTIONS. 1993;6(1):55-65.
MULLER, P., KELLER, M., WENG, W. M., QUANDT, J., Arnold, W., & Pühler, A. (1993). GENETIC-ANALYSIS OF THE RHIZOBIUM-MELILOTI EXOYFQ OPERON - EXOY IS HOMOLOGOUS TO SUGAR TRANSFERASES AND EXOQ REPRESENTS A TRANSMEMBRANE PROTEIN. MOLECULAR PLANT-MICROBE INTERACTIONS, 6(1), 55-65. doi:10.1094/MPMI-6-055
MULLER, P., KELLER, M., WENG, W. M., QUANDT, J., Arnold, W., and Pühler, A. (1993). GENETIC-ANALYSIS OF THE RHIZOBIUM-MELILOTI EXOYFQ OPERON - EXOY IS HOMOLOGOUS TO SUGAR TRANSFERASES AND EXOQ REPRESENTS A TRANSMEMBRANE PROTEIN. MOLECULAR PLANT-MICROBE INTERACTIONS 6, 55-65.
MULLER, P., et al., 1993. GENETIC-ANALYSIS OF THE RHIZOBIUM-MELILOTI EXOYFQ OPERON - EXOY IS HOMOLOGOUS TO SUGAR TRANSFERASES AND EXOQ REPRESENTS A TRANSMEMBRANE PROTEIN. MOLECULAR PLANT-MICROBE INTERACTIONS, 6(1), p 55-65.
P. MULLER, et al., “GENETIC-ANALYSIS OF THE RHIZOBIUM-MELILOTI EXOYFQ OPERON - EXOY IS HOMOLOGOUS TO SUGAR TRANSFERASES AND EXOQ REPRESENTS A TRANSMEMBRANE PROTEIN”, MOLECULAR PLANT-MICROBE INTERACTIONS, vol. 6, 1993, pp. 55-65.
MULLER, P., KELLER, M., WENG, W.M., QUANDT, J., Arnold, W., Pühler, A.: GENETIC-ANALYSIS OF THE RHIZOBIUM-MELILOTI EXOYFQ OPERON - EXOY IS HOMOLOGOUS TO SUGAR TRANSFERASES AND EXOQ REPRESENTS A TRANSMEMBRANE PROTEIN. MOLECULAR PLANT-MICROBE INTERACTIONS. 6, 55-65 (1993).
MULLER, P, KELLER, M, WENG, WM, QUANDT, J, Arnold, Walter, and Pühler, Alfred. “GENETIC-ANALYSIS OF THE RHIZOBIUM-MELILOTI EXOYFQ OPERON - EXOY IS HOMOLOGOUS TO SUGAR TRANSFERASES AND EXOQ REPRESENTS A TRANSMEMBRANE PROTEIN”. MOLECULAR PLANT-MICROBE INTERACTIONS 6.1 (1993): 55-65.

35 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Mechanism of bacitracin resistance in gram-negative bacteria that synthesize exopolysaccharides.
Pollock TJ, Thorne L, Yamazaki M, Mikolajczak MJ, Armentrout RW., J Bacteriol 176(20), 1994
PMID: 7928993
Nucleotide sequence analysis of genes essential for capsular polysaccharide biosynthesis in Streptococcus pneumoniae type 19F.
Guidolin A, Morona JK, Morona R, Hansman D, Paton JC., Infect Immun 62(12), 1994
PMID: 7960118
Family of glycosyl transferases needed for the synthesis of succinoglycan by Rhizobium meliloti.
Glucksmann MA, Reuber TL, Walker GC., J Bacteriol 175(21), 1993
PMID: 8226645

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 8439670
PubMed | Europe PMC

Suchen in

Google Scholar