Analysis of the Rhizobium meliloti exoH/exoK/exoL fragment: ExoK shows homology to excreted endo-beta-1,3-1,4-glucanases and ExoH resembles membrane proteins

BECKER A, KLEICKMANN A, Arnold W, Pühler A (1993)
Mol Gen Genet 238(1-2): 145-154.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Nucleotide sequencing of a 4.15 kb DNA fragment from megaplasmid 2 of Rhizobium meliloti 2011 revealed the location of the genes exoH, exoK and exoL. The putative proteins encoded by these genes have molecular weights of 41, 30, and 44 kDa, respectively. The hydrophobicity profile of the ExoH amino acid sequence resembles that of transmembrane proteins. The predicted exoL gene product does not contain hydrophobic regions, indicating a cytoplasmic localization. The exoK gene product is characterized by a putative signal peptide and exhibits significant homology to endo-beta-1,3-1,4-glucanases of bacilli and Clostridium thermocellum. R. meliloti exoK mutants induced pink nodules and synthesized a reduced amount of exopolysaccharide (EPS). Colonies of this mutant showed a delay in the appearance of the Calcofluor white fluorescence. In addition, the formation of the characteristic halo was strongly delayed. R. meliloti exoL and exoH mutants induced pseudonodules. The exoH, but not the exoL mutant, synthesized an EPS that could be precipitated by cetyl pyridinium chloride (CPC) and also by ethanol. Plasmid integration mutagenesis revealed promoter regions preceding exoH, exoK and exoL.
Erscheinungsjahr
Zeitschriftentitel
Mol Gen Genet
Band
238
Ausgabe
1-2
Seite(n)
145-154
ISSN
PUB-ID

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BECKER A, KLEICKMANN A, Arnold W, Pühler A. Analysis of the Rhizobium meliloti exoH/exoK/exoL fragment: ExoK shows homology to excreted endo-beta-1,3-1,4-glucanases and ExoH resembles membrane proteins. Mol Gen Genet. 1993;238(1-2):145-154.
BECKER, A., KLEICKMANN, A., Arnold, W., & Pühler, A. (1993). Analysis of the Rhizobium meliloti exoH/exoK/exoL fragment: ExoK shows homology to excreted endo-beta-1,3-1,4-glucanases and ExoH resembles membrane proteins. Mol Gen Genet, 238(1-2), 145-154.
BECKER, A., KLEICKMANN, A., Arnold, W., and Pühler, A. (1993). Analysis of the Rhizobium meliloti exoH/exoK/exoL fragment: ExoK shows homology to excreted endo-beta-1,3-1,4-glucanases and ExoH resembles membrane proteins. Mol Gen Genet 238, 145-154.
BECKER, A., et al., 1993. Analysis of the Rhizobium meliloti exoH/exoK/exoL fragment: ExoK shows homology to excreted endo-beta-1,3-1,4-glucanases and ExoH resembles membrane proteins. Mol Gen Genet, 238(1-2), p 145-154.
A. BECKER, et al., “Analysis of the Rhizobium meliloti exoH/exoK/exoL fragment: ExoK shows homology to excreted endo-beta-1,3-1,4-glucanases and ExoH resembles membrane proteins”, Mol Gen Genet, vol. 238, 1993, pp. 145-154.
BECKER, A., KLEICKMANN, A., Arnold, W., Pühler, A.: Analysis of the Rhizobium meliloti exoH/exoK/exoL fragment: ExoK shows homology to excreted endo-beta-1,3-1,4-glucanases and ExoH resembles membrane proteins. Mol Gen Genet. 238, 145-154 (1993).
BECKER, A, KLEICKMANN, A, Arnold, Walter, and Pühler, Alfred. “Analysis of the Rhizobium meliloti exoH/exoK/exoL fragment: ExoK shows homology to excreted endo-beta-1,3-1,4-glucanases and ExoH resembles membrane proteins”. Mol Gen Genet 238.1-2 (1993): 145-154.

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Piacente F, De Castro C, Jeudy S, Molinaro A, Salis A, Damonte G, Bernardi C, Abergel C, Tonetti MG., J Biol Chem 289(35), 2014
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Glenn SA, Gurich N, Feeney MA, González JE., J Bacteriol 189(19), 2007
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Skorupska A, Janczarek M, Marczak M, Mazur A, Król J., Microb Cell Fact 5(), 2006
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Environmental regulation of exopolysaccharide production in Sinorhizobium meliloti.
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PMID: 10633091
The mdoC gene of Escherichia coli encodes a membrane protein that is required for succinylation of osmoregulated periplasmic glucans.
Lacroix JM, Lanfroy E, Cogez V, Lequette Y, Bohin A, Bohin JP., J Bacteriol 181(12), 1999
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The 32-kilobase exp gene cluster of Rhizobium meliloti directing the biosynthesis of galactoglucan: genetic organization and properties of the encoded gene products.
Becker A, Rüberg S, Küster H, Roxlau AA, Keller M, Ivashina T, Cheng HP, Walker GC, Pühler A., J Bacteriol 179(4), 1997
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Rhizobium extracellular structures in the symbiosis.
Coronado C, Sánchez-Andújar B, Palomares AJ., World J Microbiol Biotechnol 12(2), 1996
PMID: 24415160
Low molecular weight EPS II of Rhizobium meliloti allows nodule invasion in Medicago sativa.
González JE, Reuhs BL, Walker GC., Proc Natl Acad Sci U S A 93(16), 1996
PMID: 8710923
Rhizobium meliloti exopolysaccharides: synthesis and symbiotic function.
González JE, York GM, Walker GC., Gene 179(1), 1996
PMID: 8955640
Effect of Phosphate Limitation on Synthesis of Periplasmic Cyclic (beta)-(1,2)-Glucans.
Breedveld MW, Benesi AJ, Marco ML, Miller KJ., Appl Environ Microbiol 61(3), 1995
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A new gene required for cellulose production and a gene encoding cellulolytic activity in Acetobacter xylinum are colocalized with the bcs operon.
Standal R, Iversen TG, Coucheron DH, Fjaervik E, Blatny JM, Valla S., J Bacteriol 176(3), 1994
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Cloning and sequencing of a Rhodothermus marinus gene, bglA, coding for a thermostable beta-glucanase and its expression in Escherichia coli.
Spilliaert R, Hreggvidsson GO, Kristjansson JK, Eggertsson G, Palsdottir A., Eur J Biochem 224(3), 1994
PMID: 7925416
Family of glycosyl transferases needed for the synthesis of succinoglycan by Rhizobium meliloti.
Glucksmann MA, Reuber TL, Walker GC., J Bacteriol 175(21), 1993
PMID: 8226645

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