A HIGHLY SENSITIVE IMMUNOENZYMOMETRIC ASSAY FOR THE DETERMINATION OF ANGIOGENIN

BLASER J, TRIEBEL S, KOPP C, Tschesche H (1993)
EUROPEAN JOURNAL OF CLINICAL CHEMISTRY AND CLINICAL BIOCHEMISTRY 31(8): 513-516.

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Abstract
A polyclonal antibody to human recombinant angiogenin was prepared in rabbits using a Pam3CysSerGly conjugate. The antibody was then used to develop the first highly sensitive enzyme-labelled immunometric assay for this vascularisation inducing and tumour associated protein. The assay was suitable for quantification of angiogenin in body fluids between 2.5 and 0.05 mug/l. The mean intra-assay imprecision was 6.0% and the inter-assay imprecision 7.9%. Angiogenin in human plasma was found to lie in the range of 0.38 to 0.11 mg/l with a mean of 0.25 +/- 0.07 mg/l.
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BLASER J, TRIEBEL S, KOPP C, Tschesche H. A HIGHLY SENSITIVE IMMUNOENZYMOMETRIC ASSAY FOR THE DETERMINATION OF ANGIOGENIN. EUROPEAN JOURNAL OF CLINICAL CHEMISTRY AND CLINICAL BIOCHEMISTRY. 1993;31(8):513-516.
BLASER, J., TRIEBEL, S., KOPP, C., & Tschesche, H. (1993). A HIGHLY SENSITIVE IMMUNOENZYMOMETRIC ASSAY FOR THE DETERMINATION OF ANGIOGENIN. EUROPEAN JOURNAL OF CLINICAL CHEMISTRY AND CLINICAL BIOCHEMISTRY, 31(8), 513-516.
BLASER, J., TRIEBEL, S., KOPP, C., and Tschesche, H. (1993). A HIGHLY SENSITIVE IMMUNOENZYMOMETRIC ASSAY FOR THE DETERMINATION OF ANGIOGENIN. EUROPEAN JOURNAL OF CLINICAL CHEMISTRY AND CLINICAL BIOCHEMISTRY 31, 513-516.
BLASER, J., et al., 1993. A HIGHLY SENSITIVE IMMUNOENZYMOMETRIC ASSAY FOR THE DETERMINATION OF ANGIOGENIN. EUROPEAN JOURNAL OF CLINICAL CHEMISTRY AND CLINICAL BIOCHEMISTRY, 31(8), p 513-516.
J. BLASER, et al., “A HIGHLY SENSITIVE IMMUNOENZYMOMETRIC ASSAY FOR THE DETERMINATION OF ANGIOGENIN”, EUROPEAN JOURNAL OF CLINICAL CHEMISTRY AND CLINICAL BIOCHEMISTRY, vol. 31, 1993, pp. 513-516.
BLASER, J., TRIEBEL, S., KOPP, C., Tschesche, H.: A HIGHLY SENSITIVE IMMUNOENZYMOMETRIC ASSAY FOR THE DETERMINATION OF ANGIOGENIN. EUROPEAN JOURNAL OF CLINICAL CHEMISTRY AND CLINICAL BIOCHEMISTRY. 31, 513-516 (1993).
BLASER, J, TRIEBEL, S, KOPP, C, and Tschesche, Harald. “A HIGHLY SENSITIVE IMMUNOENZYMOMETRIC ASSAY FOR THE DETERMINATION OF ANGIOGENIN”. EUROPEAN JOURNAL OF CLINICAL CHEMISTRY AND CLINICAL BIOCHEMISTRY 31.8 (1993): 513-516.
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Hu GF, Riordan JF, Vallee BL., Proc. Natl. Acad. Sci. U.S.A. 94(6), 1997
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