DIRECT ACTIVATION OF HUMAN NEUTROPHIL PROCOLLAGENASE BY RECOMBINANT STROMELYSIN

KNAUPER V, WILHELM SM, SEPERACK PK, DECLERCK YA, LANGLEY KE, OSTHUES A, Tschesche H (1993)
BIOCHEMICAL JOURNAL 295: 581-586.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ; ;
Abstract
Human neutrophil procollagenase was activated by incubation with recombinant active stromelysin. Activation was achieved by cleavage of the Gly78-Phe79 peptide bond at the end of the propeptide domain in a single-step activation mechanism. In addition, accelerated activation was achieved when N-terminally truncated, latent collagenase (with Phe49 as its N-terminal residue) was incubated with recombinant active stromelysin. Determination of the specific activity of recombinant-stromelysin-activated neutrophil collagenase with dinitrophenyl-octapeptide or type I collagen demonstrated the generation of high specific activity. The specific activity of stromelysin-activated enzyme was considerably higher than that of trypsin- or HgCl2-activated collagenase. Thus human neutrophil collagenase is superactivated, like the homologous fibroblast collagenase [Murphy, Cockett, Stephens, Smith and Docherty (1987) Biochem. J. 248, 265-268]. The occurrence of Phe79 at the N-terminus of the neutrophil collagenase seemed to be critical for superactivation, which is in agreement with data published Suzuki. Enghild, Morodomi, Salvesen and Nagase [(1990) Biochemistry 29. 10261-10270] on fibroblast collagenase.
Publishing Year
ISSN
PUB-ID

Cite this

KNAUPER V, WILHELM SM, SEPERACK PK, et al. DIRECT ACTIVATION OF HUMAN NEUTROPHIL PROCOLLAGENASE BY RECOMBINANT STROMELYSIN. BIOCHEMICAL JOURNAL. 1993;295:581-586.
KNAUPER, V., WILHELM, S. M., SEPERACK, P. K., DECLERCK, Y. A., LANGLEY, K. E., OSTHUES, A., & Tschesche, H. (1993). DIRECT ACTIVATION OF HUMAN NEUTROPHIL PROCOLLAGENASE BY RECOMBINANT STROMELYSIN. BIOCHEMICAL JOURNAL, 295, 581-586.
KNAUPER, V., WILHELM, S. M., SEPERACK, P. K., DECLERCK, Y. A., LANGLEY, K. E., OSTHUES, A., and Tschesche, H. (1993). DIRECT ACTIVATION OF HUMAN NEUTROPHIL PROCOLLAGENASE BY RECOMBINANT STROMELYSIN. BIOCHEMICAL JOURNAL 295, 581-586.
KNAUPER, V., et al., 1993. DIRECT ACTIVATION OF HUMAN NEUTROPHIL PROCOLLAGENASE BY RECOMBINANT STROMELYSIN. BIOCHEMICAL JOURNAL, 295, p 581-586.
V. KNAUPER, et al., “DIRECT ACTIVATION OF HUMAN NEUTROPHIL PROCOLLAGENASE BY RECOMBINANT STROMELYSIN”, BIOCHEMICAL JOURNAL, vol. 295, 1993, pp. 581-586.
KNAUPER, V., WILHELM, S.M., SEPERACK, P.K., DECLERCK, Y.A., LANGLEY, K.E., OSTHUES, A., Tschesche, H.: DIRECT ACTIVATION OF HUMAN NEUTROPHIL PROCOLLAGENASE BY RECOMBINANT STROMELYSIN. BIOCHEMICAL JOURNAL. 295, 581-586 (1993).
KNAUPER, V, WILHELM, SM, SEPERACK, PK, DECLERCK, YA, LANGLEY, KE, OSTHUES, A, and Tschesche, Harald. “DIRECT ACTIVATION OF HUMAN NEUTROPHIL PROCOLLAGENASE BY RECOMBINANT STROMELYSIN”. BIOCHEMICAL JOURNAL 295 (1993): 581-586.
This data publication is cited in the following publications:
This publication cites the following data publications:

58 Citations in Europe PMC

Data provided by Europe PubMed Central.

Chitosan enriched three-dimensional matrix reduces inflammatory and catabolic mediators production by human chondrocytes.
Oprenyeszk F, Sanchez C, Dubuc JE, Maquet V, Henrist C, Compere P, Henrotin Y., PLoS ONE 10(5), 2015
PMID: 26020773
MMP-1 and -3 haplotype is associated with congenital anomalies of the kidney and urinary tract.
Djuric T, Zivkovic M, Milosevic B, Andjelevski M, Cvetkovic M, Kostic M, Stankovic A., Pediatr. Nephrol. 29(5), 2014
PMID: 24414606
Relaxin decreases the severity of established hepatic fibrosis in mice.
Bennett RG, Heimann DG, Singh S, Simpson RL, Tuma DJ., Liver Int. 34(3), 2014
PMID: 23870027
Collagen I regulates matrix metalloproteinase-2 activation in osteosarcoma cells independent of S100A4.
Elenjord R, Allen JB, Johansen HT, Kildalsen H, Svineng G, Maelandsmo GM, Loennechen T, Winberg JO., FEBS J. 276(18), 2009
PMID: 19682073
Immunological investigation of the hepatic tissue from infants with biliary atresia.
Baba H, Ohtsuka Y, Fujii T, Haruna H, Nagata S, Kobayashi H, Yamataka A, Shimizu T, Miyano T, Yamashiro Y., Pediatr. Surg. Int. 25(2), 2009
PMID: 19089432

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 8240261
PubMed | Europe PMC

Search this title in

Google Scholar