SATURATION MUTAGENESIS IN ESCHERICHIA-COLI OF A CLONED XANTHOMONAS-CAMPESTRIS DNA FRAGMENT WITH THE LUX TRANSPOSON TN4431 USING THE DELIVERY PLASMID PDS1, THERMOSENSITIVE IN REPLICATION

STEINMANN D, WIGGERICH HG, KLAUKE B, SCHRAMM U, Pühler A, PRIEFER UB (1993)
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 40(2-3): 356-360.

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A system allowing transposon mutagenesis of cloned DNA fragments in Escherichia coil with Tn4431, which carries the promotorless luciferase (lux) operon of Vibrio fischeri, has been developed. The transposon delivery plasmid, pDS1, based on an IncF replicon, is thermosensitive in replication and mobilizable to many Gram-negative bacteria. We used pDS1 for Tn4431-saturation mutagenesis of a 10-kb DNA fragment of Xanthomonas campestris pv. campestris (X.c.c.) in E. coli and showed that the expression of the lux operon was dependent on orientation and location of the transposon. Transfer of a specific Tn4431 insertion to X.c.c. allowed the determination of the bioluminescence phenotype in planta.
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STEINMANN D, WIGGERICH HG, KLAUKE B, SCHRAMM U, Pühler A, PRIEFER UB. SATURATION MUTAGENESIS IN ESCHERICHIA-COLI OF A CLONED XANTHOMONAS-CAMPESTRIS DNA FRAGMENT WITH THE LUX TRANSPOSON TN4431 USING THE DELIVERY PLASMID PDS1, THERMOSENSITIVE IN REPLICATION. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 1993;40(2-3):356-360.
STEINMANN, D., WIGGERICH, H. G., KLAUKE, B., SCHRAMM, U., Pühler, A., & PRIEFER, U. B. (1993). SATURATION MUTAGENESIS IN ESCHERICHIA-COLI OF A CLONED XANTHOMONAS-CAMPESTRIS DNA FRAGMENT WITH THE LUX TRANSPOSON TN4431 USING THE DELIVERY PLASMID PDS1, THERMOSENSITIVE IN REPLICATION. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 40(2-3), 356-360.
STEINMANN, D., WIGGERICH, H. G., KLAUKE, B., SCHRAMM, U., Pühler, A., and PRIEFER, U. B. (1993). SATURATION MUTAGENESIS IN ESCHERICHIA-COLI OF A CLONED XANTHOMONAS-CAMPESTRIS DNA FRAGMENT WITH THE LUX TRANSPOSON TN4431 USING THE DELIVERY PLASMID PDS1, THERMOSENSITIVE IN REPLICATION. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 40, 356-360.
STEINMANN, D., et al., 1993. SATURATION MUTAGENESIS IN ESCHERICHIA-COLI OF A CLONED XANTHOMONAS-CAMPESTRIS DNA FRAGMENT WITH THE LUX TRANSPOSON TN4431 USING THE DELIVERY PLASMID PDS1, THERMOSENSITIVE IN REPLICATION. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 40(2-3), p 356-360.
D. STEINMANN, et al., “SATURATION MUTAGENESIS IN ESCHERICHIA-COLI OF A CLONED XANTHOMONAS-CAMPESTRIS DNA FRAGMENT WITH THE LUX TRANSPOSON TN4431 USING THE DELIVERY PLASMID PDS1, THERMOSENSITIVE IN REPLICATION”, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 40, 1993, pp. 356-360.
STEINMANN, D., WIGGERICH, H.G., KLAUKE, B., SCHRAMM, U., Pühler, A., PRIEFER, U.B.: SATURATION MUTAGENESIS IN ESCHERICHIA-COLI OF A CLONED XANTHOMONAS-CAMPESTRIS DNA FRAGMENT WITH THE LUX TRANSPOSON TN4431 USING THE DELIVERY PLASMID PDS1, THERMOSENSITIVE IN REPLICATION. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 40, 356-360 (1993).
STEINMANN, D, WIGGERICH, HG, KLAUKE, B, SCHRAMM, U, Pühler, Alfred, and PRIEFER, UB. “SATURATION MUTAGENESIS IN ESCHERICHIA-COLI OF A CLONED XANTHOMONAS-CAMPESTRIS DNA FRAGMENT WITH THE LUX TRANSPOSON TN4431 USING THE DELIVERY PLASMID PDS1, THERMOSENSITIVE IN REPLICATION”. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 40.2-3 (1993): 356-360.
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