LEUKOCYTE PROTEINASE RELEASE DURING STORAGE OF RED-CELL CONCENTRATES

HERTFELDER HJ, SUWER V, POPOVCENIC S, Tschesche H, HANFLAND P (1994)
EUROPEAN JOURNAL OF CLINICAL CHEMISTRY AND CLINICAL BIOCHEMISTRY 32(6): 441-447.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
The release of polymorphonuclear leukocyte proteinases in buffy-coat-depleted red cell concentrates was examined during a storage period of 35 days. Collagenase, gelatinase and elastase predominantly induce breakdown of connective tissue. However, when released by cell disintegration during red cell concentrate storage, the considerable proteolytic activities of these enzymes might influence the quality of the stored blood. During the observation period a considerable decrease in the polymorphonuclear leukocyte count was observed, accompanied by increases in the levels of collagenase, gelatinase and elastase. Compared with the enzyme levels on the day of red cell concentrate preparation, collagenase increased 20-fold, gelatinase 6-fold and elastase 100-fold during the storage period. When cells were treated with the chemoattractant hexapeptide, N-formyl-nle-leu-phe-nle-tyr-leu, and the degranulation promoting cytochalasin B, gelatinase exhibited the highest secreted concentration in the freshly prepared red cell concentrate, exceeding the maximum of spontaneously released elastase by 4- to 6-fold. However, these compounds stimulated enzyme release only during the first day after red cell concentrate preparation. Thereafter, no differences between stimulated and non-stimulated samples were observed. The data indicate that polymorphonuclear leukocytes contain a large storage pool of proteolytic enzymes. These enzymes together with other polymorphonuclear leukocyte enzymes, e.g. hydrolases and oxidoreductases, might alter the erythrocyte membrane surface and thus influence the storage quality of the prepared red cell concentrate.
Erscheinungsjahr
Zeitschriftentitel
EUROPEAN JOURNAL OF CLINICAL CHEMISTRY AND CLINICAL BIOCHEMISTRY
Band
32
Zeitschriftennummer
6
Seite
441-447
ISSN
PUB-ID

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HERTFELDER HJ, SUWER V, POPOVCENIC S, Tschesche H, HANFLAND P. LEUKOCYTE PROTEINASE RELEASE DURING STORAGE OF RED-CELL CONCENTRATES. EUROPEAN JOURNAL OF CLINICAL CHEMISTRY AND CLINICAL BIOCHEMISTRY. 1994;32(6):441-447.
HERTFELDER, H. J., SUWER, V., POPOVCENIC, S., Tschesche, H., & HANFLAND, P. (1994). LEUKOCYTE PROTEINASE RELEASE DURING STORAGE OF RED-CELL CONCENTRATES. EUROPEAN JOURNAL OF CLINICAL CHEMISTRY AND CLINICAL BIOCHEMISTRY, 32(6), 441-447.
HERTFELDER, H. J., SUWER, V., POPOVCENIC, S., Tschesche, H., and HANFLAND, P. (1994). LEUKOCYTE PROTEINASE RELEASE DURING STORAGE OF RED-CELL CONCENTRATES. EUROPEAN JOURNAL OF CLINICAL CHEMISTRY AND CLINICAL BIOCHEMISTRY 32, 441-447.
HERTFELDER, H.J., et al., 1994. LEUKOCYTE PROTEINASE RELEASE DURING STORAGE OF RED-CELL CONCENTRATES. EUROPEAN JOURNAL OF CLINICAL CHEMISTRY AND CLINICAL BIOCHEMISTRY, 32(6), p 441-447.
H.J. HERTFELDER, et al., “LEUKOCYTE PROTEINASE RELEASE DURING STORAGE OF RED-CELL CONCENTRATES”, EUROPEAN JOURNAL OF CLINICAL CHEMISTRY AND CLINICAL BIOCHEMISTRY, vol. 32, 1994, pp. 441-447.
HERTFELDER, H.J., SUWER, V., POPOVCENIC, S., Tschesche, H., HANFLAND, P.: LEUKOCYTE PROTEINASE RELEASE DURING STORAGE OF RED-CELL CONCENTRATES. EUROPEAN JOURNAL OF CLINICAL CHEMISTRY AND CLINICAL BIOCHEMISTRY. 32, 441-447 (1994).
HERTFELDER, HJ, SUWER, V, POPOVCENIC, S, Tschesche, Harald, and HANFLAND, P. “LEUKOCYTE PROTEINASE RELEASE DURING STORAGE OF RED-CELL CONCENTRATES”. EUROPEAN JOURNAL OF CLINICAL CHEMISTRY AND CLINICAL BIOCHEMISTRY 32.6 (1994): 441-447.

3 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Influence of prestorage leucocyte depletion and storage time on rheologic properties of erythrocyte concentrates.
Sollberger T, Walter R, Brand B, Contesse J, Meredith DO, Reinhart WH., Vox Sang 82(4), 2002
PMID: 12047513
Leukocyte depletion of red cell components prevents exposure of transfusion recipients to neutrophil elastase.
Willy C, Reithmeier W, Kuhlmann WD, Gerngross H, Flegel WA., Vox Sang 78(1), 2000
PMID: 10729807
Effects of leucocyte depletion on rheologic properties of human CPDA-1 blood.
Walter R, Brand B, Mark M, Schnyder L, Stifanic M, Reinhart WH., Vox Sang 79(3), 2000
PMID: 11111233

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