Subtractive cDNA cloning as a tool to analyse secondary effects of a muscle disease. Characterization of affected genes in the myotonic ADR mouse

Schleef M, Zühlke C, Schöffl F, Jockusch H (1994)
Neuromuscular Disorders 4(3): 205-217.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ;
Abstract
In myotonic ADR mice that are homozygous for a defect in the muscular chloride channel gene adr/Clc-1, the hyperexcitability of fast muscles is associated with secondary changes in gene expression and fibre type composition, cDNA clones derived from a set of genes down regulated in fast muscles of the myotonic ADR mouse were isolated by a subtractive cloning procedure. A total of 1200 clones were analysed for high expression in fast muscle of wild type and low expression in mutant mouse. Differential transcript levels were verified by northern blot hybridizations. The identities of the corresponding transcripts were determined by sequencing as myosin heavy chain IIB, alpha-tropomyosin, troponin C, a Ca2+ ATPase and parvalbumin mRNAs. Of these, mRNAs for parvalbumin and myosin heavy chain IIB were drastically downregulated in myotonic muscle (to < 10% of control). A full length cDNA clone for skeletal muscle alpha-tropomyosin was homologous to the mouse fibroblast tropomyosin isoform 2, except for the portion encoding the alpha-tropomyosin specific amino acids 258-284. A cDNA derived from the 1100 nucleotide parvalbumin transcript was cloned and the sequence for the as yet unknown 3' extended trailer, generated by alternative polyadenylation, was determined.
Publishing Year
ISSN
PUB-ID

Cite this

Schleef M, Zühlke C, Schöffl F, Jockusch H. Subtractive cDNA cloning as a tool to analyse secondary effects of a muscle disease. Characterization of affected genes in the myotonic ADR mouse. Neuromuscular Disorders. 1994;4(3):205-217.
Schleef, M., Zühlke, C., Schöffl, F., & Jockusch, H. (1994). Subtractive cDNA cloning as a tool to analyse secondary effects of a muscle disease. Characterization of affected genes in the myotonic ADR mouse. Neuromuscular Disorders, 4(3), 205-217.
Schleef, M., Zühlke, C., Schöffl, F., and Jockusch, H. (1994). Subtractive cDNA cloning as a tool to analyse secondary effects of a muscle disease. Characterization of affected genes in the myotonic ADR mouse. Neuromuscular Disorders 4, 205-217.
Schleef, M., et al., 1994. Subtractive cDNA cloning as a tool to analyse secondary effects of a muscle disease. Characterization of affected genes in the myotonic ADR mouse. Neuromuscular Disorders, 4(3), p 205-217.
M. Schleef, et al., “Subtractive cDNA cloning as a tool to analyse secondary effects of a muscle disease. Characterization of affected genes in the myotonic ADR mouse”, Neuromuscular Disorders, vol. 4, 1994, pp. 205-217.
Schleef, M., Zühlke, C., Schöffl, F., Jockusch, H.: Subtractive cDNA cloning as a tool to analyse secondary effects of a muscle disease. Characterization of affected genes in the myotonic ADR mouse. Neuromuscular Disorders. 4, 205-217 (1994).
Schleef, M., Zühlke, C., Schöffl, F., and Jockusch, Harald. “Subtractive cDNA cloning as a tool to analyse secondary effects of a muscle disease. Characterization of affected genes in the myotonic ADR mouse”. Neuromuscular Disorders 4.3 (1994): 205-217.
This data publication is cited in the following publications:
This publication cites the following data publications:

4 Citations in Europe PMC

Data provided by Europe PubMed Central.

Identification of secondary effects of hyperexcitability by proteomic profiling of myotonic mouse muscle.
Staunton L, Jockusch H, Wiegand C, Albrecht T, Ohlendieck K., Mol Biosyst 7(8), 2011
PMID: 21629954
Metal-ion affinity and specificity in EF-hand proteins: coordination geometry and domain plasticity in parvalbumin.
Cates MS, Berry MB, Ho EL, Li Q, Potter JD, Phillips GN Jr., Structure 7(10), 1999
PMID: 10545326

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 7522680
PubMed | Europe PMC

Search this title in

Google Scholar