NEW EVIDENCE FOR DIMERIZATION-DEPENDENCE OF THE ACTIVITY OF CANDIDA-RUGOSA LIPASE

Flaschel E, SCHAFFER T (1994)
In: BIOCATALYSIS. 9. HARWOOD ACAD PUBL GMBH: 241-248.

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Abstract
The specific activity of the lipase of Candida rugosa decreases with increasing enzyme concentration even in the presence of soluble substrates. Data about the hydrolysis of 2-chloropropionic acid ethyl ester (CPEE) had suggested that this phenomenon may be caused either by dimerization of the lipase or by adsorption onto the reactor wall. In order to distinguish between both models, experiments were carried out by changing not only the enzyme concentration but also the wetted surface area of the reaction vessel. These novel data reveal that wetted glass surfaces are of only minor importance - if any. Thus, the decrease of activity seems to be caused by some kind of dimerization of the lipase. In addition, it is shown that adsorption onto hydrophobic surfaces can have a dramatic effect on the specific activity. In the presence of large hydrophobic surface areas the specific activity is found to be almost as high as that observed in the presence of insoluble substrate. The analysis of a commonly used test system for lipase activity measurements based on triacetin hydrolysis exhibits a similar activity-enzyme concentration dependence.
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Flaschel E, SCHAFFER T. NEW EVIDENCE FOR DIMERIZATION-DEPENDENCE OF THE ACTIVITY OF CANDIDA-RUGOSA LIPASE. In: BIOCATALYSIS. Vol 9. HARWOOD ACAD PUBL GMBH; 1994: 241-248.
Flaschel, E., & SCHAFFER, T. (1994). NEW EVIDENCE FOR DIMERIZATION-DEPENDENCE OF THE ACTIVITY OF CANDIDA-RUGOSA LIPASE. BIOCATALYSIS, 9(1-4), 241-248.
Flaschel, E., and SCHAFFER, T. (1994). “NEW EVIDENCE FOR DIMERIZATION-DEPENDENCE OF THE ACTIVITY OF CANDIDA-RUGOSA LIPASE” in BIOCATALYSIS, vol. 9, (HARWOOD ACAD PUBL GMBH), 241-248.
Flaschel, E., & SCHAFFER, T., 1994. NEW EVIDENCE FOR DIMERIZATION-DEPENDENCE OF THE ACTIVITY OF CANDIDA-RUGOSA LIPASE. In BIOCATALYSIS. no.9 HARWOOD ACAD PUBL GMBH, pp. 241-248.
E. Flaschel and T. SCHAFFER, “NEW EVIDENCE FOR DIMERIZATION-DEPENDENCE OF THE ACTIVITY OF CANDIDA-RUGOSA LIPASE”, BIOCATALYSIS, vol. 9, HARWOOD ACAD PUBL GMBH, 1994, pp.241-248.
Flaschel, E., SCHAFFER, T.: NEW EVIDENCE FOR DIMERIZATION-DEPENDENCE OF THE ACTIVITY OF CANDIDA-RUGOSA LIPASE. BIOCATALYSIS. 9, p. 241-248. HARWOOD ACAD PUBL GMBH (1994).
Flaschel, Erwin, and SCHAFFER, T. “NEW EVIDENCE FOR DIMERIZATION-DEPENDENCE OF THE ACTIVITY OF CANDIDA-RUGOSA LIPASE”. BIOCATALYSIS. HARWOOD ACAD PUBL GMBH, 1994.Vol. 9. 241-248.
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