IN-VIVO INHIBITION OF HUMAN NEUTROPHIL COLLAGENASE (MMP-8) ACTIVITY DURING LONG-TERM COMBINATION THERAPY OF DOXYCYCLINE AND NONSTEROIDAL ANTIINFLAMMATORY DRUGS (NSAID) IN ACUTE REACTIVE ARTHRITIS

LAUHIO A, SALO T, DING Y, KONTTINEN YT, NORDSTROM D, Tschesche H, LAHDEVIRTA J, GOLUB LM, SORSA T (1994)
CLINICAL AND EXPERIMENTAL IMMUNOLOGY 98(1): 21-28.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ; ; ; ;
Abstract
We studied the in vivo effect of long-term doxycycline treatment combined with NSAID on human interstitial collagenases, other matrix metalloproteinases, serine proteinases, tissue inhibitor of matrix metalloproteinase-l (TIMP-1) and lactoferrin from saliva and serum during the course of acute reactive arthritis (ReA). Collagenase activity and serine proteases (elastase-like, cathepsin G-like and trypsin-like activities) of saliva (n = 10) and gelatinase, lactoferrin and TIMP-1 of saliva (E = 10) and serum (n = 10) samples before and after 2 months doxycycline treatment, combined with NSAID, were studied by quantitative SDS-PAGE assay, ELISA assay and by spectrophotometric assay. The cellular source and molecular forms of salivary collagenase were characterized by immunoblotting using specific antisera. We found that activities of total and endogenously active interstitial collagenase reduced significantly. The salivary collagenase was found to originate from neutrophils. No fragmentation of either pro 75-kD and active 65-kD MMP-8 was detected after 2 months doxycycline treatment. However, during 2 months doxycycline and NSAID treatment no reduction of salivary and serum gelatinase, lactoferrin and TIMP-1-levels and salivary serine protease activities were detected. The in vivo inhibition of collagenase (MMP-8) activity during long-term doxycycline therapy in human saliva containing inflammatory exudate of ReA patients may contribute to the reduced tissue destruction observed in recent clinical and animal model studies in arthritides during long-term doxycycline/tetracycline treatment.
Publishing Year
ISSN
PUB-ID

Cite this

LAUHIO A, SALO T, DING Y, et al. IN-VIVO INHIBITION OF HUMAN NEUTROPHIL COLLAGENASE (MMP-8) ACTIVITY DURING LONG-TERM COMBINATION THERAPY OF DOXYCYCLINE AND NONSTEROIDAL ANTIINFLAMMATORY DRUGS (NSAID) IN ACUTE REACTIVE ARTHRITIS. CLINICAL AND EXPERIMENTAL IMMUNOLOGY. 1994;98(1):21-28.
LAUHIO, A., SALO, T., DING, Y., KONTTINEN, Y. T., NORDSTROM, D., Tschesche, H., LAHDEVIRTA, J., et al. (1994). IN-VIVO INHIBITION OF HUMAN NEUTROPHIL COLLAGENASE (MMP-8) ACTIVITY DURING LONG-TERM COMBINATION THERAPY OF DOXYCYCLINE AND NONSTEROIDAL ANTIINFLAMMATORY DRUGS (NSAID) IN ACUTE REACTIVE ARTHRITIS. CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 98(1), 21-28.
LAUHIO, A., SALO, T., DING, Y., KONTTINEN, Y. T., NORDSTROM, D., Tschesche, H., LAHDEVIRTA, J., GOLUB, L. M., and SORSA, T. (1994). IN-VIVO INHIBITION OF HUMAN NEUTROPHIL COLLAGENASE (MMP-8) ACTIVITY DURING LONG-TERM COMBINATION THERAPY OF DOXYCYCLINE AND NONSTEROIDAL ANTIINFLAMMATORY DRUGS (NSAID) IN ACUTE REACTIVE ARTHRITIS. CLINICAL AND EXPERIMENTAL IMMUNOLOGY 98, 21-28.
LAUHIO, A., et al., 1994. IN-VIVO INHIBITION OF HUMAN NEUTROPHIL COLLAGENASE (MMP-8) ACTIVITY DURING LONG-TERM COMBINATION THERAPY OF DOXYCYCLINE AND NONSTEROIDAL ANTIINFLAMMATORY DRUGS (NSAID) IN ACUTE REACTIVE ARTHRITIS. CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 98(1), p 21-28.
A. LAUHIO, et al., “IN-VIVO INHIBITION OF HUMAN NEUTROPHIL COLLAGENASE (MMP-8) ACTIVITY DURING LONG-TERM COMBINATION THERAPY OF DOXYCYCLINE AND NONSTEROIDAL ANTIINFLAMMATORY DRUGS (NSAID) IN ACUTE REACTIVE ARTHRITIS”, CLINICAL AND EXPERIMENTAL IMMUNOLOGY, vol. 98, 1994, pp. 21-28.
LAUHIO, A., SALO, T., DING, Y., KONTTINEN, Y.T., NORDSTROM, D., Tschesche, H., LAHDEVIRTA, J., GOLUB, L.M., SORSA, T.: IN-VIVO INHIBITION OF HUMAN NEUTROPHIL COLLAGENASE (MMP-8) ACTIVITY DURING LONG-TERM COMBINATION THERAPY OF DOXYCYCLINE AND NONSTEROIDAL ANTIINFLAMMATORY DRUGS (NSAID) IN ACUTE REACTIVE ARTHRITIS. CLINICAL AND EXPERIMENTAL IMMUNOLOGY. 98, 21-28 (1994).
LAUHIO, A, SALO, T, DING, Y, KONTTINEN, YT, NORDSTROM, D, Tschesche, Harald, LAHDEVIRTA, J, GOLUB, LM, and SORSA, T. “IN-VIVO INHIBITION OF HUMAN NEUTROPHIL COLLAGENASE (MMP-8) ACTIVITY DURING LONG-TERM COMBINATION THERAPY OF DOXYCYCLINE AND NONSTEROIDAL ANTIINFLAMMATORY DRUGS (NSAID) IN ACUTE REACTIVE ARTHRITIS”. CLINICAL AND EXPERIMENTAL IMMUNOLOGY 98.1 (1994): 21-28.
This data publication is cited in the following publications:
This publication cites the following data publications:

27 Citations in Europe PMC

Data provided by Europe PubMed Central.

Do salivary and serum collagenases have a role in an association between obstructive sleep apnea syndrome and periodontal disease? A preliminary case-control study.
Nizam N, Basoglu OK, Tasbakan MS, Holthofer A, Tervahartiala T, Sorsa T, Buduneli N., Arch. Oral Biol. 60(1), 2015
PMID: 25455127

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 7923879
PubMed | Europe PMC

Search this title in

Google Scholar