Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli

Tauch A, Kirchner O, Wehmeier L, Kalinowski J, Pühler A (1994)
FEMS MICROBIOLOGY LETTERS 123(3): 343-347.

Journal Article | Published | English

No fulltext has been uploaded

Author
Abstract
Efficient electroporation of Escherichia coli with plasmid DNA isolated from Corynebacterium glutamicum depends on the use of Mcr-deficient E. coli strains. The transformation frequency increased nearly 800-fold when the Mcr-deficient E. coli DH5 alpha MCR was used instead of E. coli DH5 alpha. We used E. coil strains with different mutations in the methyl-specific restriction systems to show that McrBC-deficiency is sufficient to generate this effect. The results imply that C. glutamicum DNA contains methylcytosine in specific sequences recognized by the E. coli McrBC system.
Publishing Year
ISSN
PUB-ID

Cite this

Tauch A, Kirchner O, Wehmeier L, Kalinowski J, Pühler A. Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli. FEMS MICROBIOLOGY LETTERS. 1994;123(3):343-347.
Tauch, A., Kirchner, O., Wehmeier, L., Kalinowski, J., & Pühler, A. (1994). Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli. FEMS MICROBIOLOGY LETTERS, 123(3), 343-347.
Tauch, A., Kirchner, O., Wehmeier, L., Kalinowski, J., and Pühler, A. (1994). Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli. FEMS MICROBIOLOGY LETTERS 123, 343-347.
Tauch, A., et al., 1994. Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli. FEMS MICROBIOLOGY LETTERS, 123(3), p 343-347.
A. Tauch, et al., “Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli”, FEMS MICROBIOLOGY LETTERS, vol. 123, 1994, pp. 343-347.
Tauch, A., Kirchner, O., Wehmeier, L., Kalinowski, J., Pühler, A.: Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli. FEMS MICROBIOLOGY LETTERS. 123, 343-347 (1994).
Tauch, Andreas, Kirchner, O, Wehmeier, L, Kalinowski, Jörn, and Pühler, Alfred. “Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli”. FEMS MICROBIOLOGY LETTERS 123.3 (1994): 343-347.
This data publication is cited in the following publications:
This publication cites the following data publications:

33 Citations in Europe PMC

Data provided by Europe PubMed Central.

Isolation of Genetically Tractable Most-Wanted Bacteria by Metaparental Mating.
Cuiv PO, Smith WJ, Pottenger S, Burman S, Shanahan ER, Morrison M., Sci Rep 5(), 2015
PMID: 26293474
A highly efficient transposon mutagenesis system for the tomato pathogen Clavibacter michiganensis subsp. michiganensis.
Kirchner O, Gartemann KH, Zellermann EM, Eichenlaub R, Burger A., Mol. Plant Microbe Interact. 14(11), 2001
PMID: 11763129
Analysis of nucleotide methylation in DNA from Corynebacterium glutamicum and related species.
Jang KH, Chambers PJ, Britz ML., FEMS Microbiol. Lett. 136(3), 1996
PMID: 8867385

15 References

Data provided by Europe PubMed Central.

Biology of DNA restriction.
Bickle TA, Kruger DH., Microbiol. Rev. 57(2), 1993
PMID: 8336674
Characterization and expression of the Escherichia coli Mrr restriction system.
Waite-Rees PA, Keating CJ, Moran LS, Slatko BE, Hornstra LJ, Benner JS., J. Bacteriol. 173(16), 1991
PMID: 1650347

AUTHOR UNKNOWN, 0

AUTHOR UNKNOWN, 0
High-frequency conjugal plasmid transfer from gram-negative Escherichia coli to various gram-positive coryneform bacteria.
Schafer A, Kalinowski J, Simon R, Seep-Feldhaus AH, Puhler A., J. Bacteriol. 172(3), 1990
PMID: 2106514
Manipulation of Corynebacterium glutamicum by gene disruption and replacement.
Schwarzer A, Puhler A., Biotechnology (N.Y.) 9(1), 1991
PMID: 1367217

AUTHOR UNKNOWN, 0
McrA and McrB restriction phenotypes of some E. coli strains and implications for gene cloning.
Raleigh EA, Murray NE, Revel H, Blumenthal RM, Westaway D, Reith AD, Rigby PW, Elhai J, Hanahan D., Nucleic Acids Res. 16(4), 1988
PMID: 2831502
New restriction-minus derivates of XL1-Blue E. coli cells
Jerpseth, Strategies 6(), 1993
High efficiency transformation of E. coli by high voltage electroporation.
Dower WJ, Miller JF, Ragsdale CW., Nucleic Acids Res. 16(13), 1988
PMID: 3041370
Characterization of an endogenous plasmid and development of cloning vectors and a transformation system in Brevibacterium lactofermentum
Santamaria, J. Gen. Microbiol. 130(), 1984

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 7988915
PubMed | Europe PMC

Search this title in

Google Scholar