NEUTROPHIL COLLAGENASE (MMP-8) CLEAVES AT THE AGGRECANASE SITE E(373)-A(374) IN THE INTERGLOBULAR DOMAIN OF CARTILAGE AGGRECAN

FOSANG AJ, LAST K, NEAME PJ, MURPHY G, KNAUPER V, Tschesche H, HUGHES CE, CATERSON B, HARDINGHAM TE (1994)
BIOCHEMICAL JOURNAL 304: 347-351.

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Native and recombinant neutrophil collagenase (MMP-8) was shown to cleave at the E(373)-A(374) 'aggrecanase'' site in the interglobular domain of aggrecan. The time course of digestion in vitro showed that MMP-8 cleaved initially at N-341-F-342, the predominant metalloproteinase site, before cleaving at the E(373)-A(374) site. A synthetic peptide, IPENFFG, inhibited cleavage at E(373)-A(374) but not N-341-F-342 in vitro, indicating that the E(373)-A(374) sequence was a less preferred site for MMP-8 cleavage than N-341-F-342. IPENFFG also inhibited release of A(374) RGSVI fragments from cartilage in explant culture, suggesting that a metalloproteinase cleaved at the aggrecanase site in situ. The possibility remains that 'aggrecanase' may be a metalloproteinase in cartilage.
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FOSANG AJ, LAST K, NEAME PJ, et al. NEUTROPHIL COLLAGENASE (MMP-8) CLEAVES AT THE AGGRECANASE SITE E(373)-A(374) IN THE INTERGLOBULAR DOMAIN OF CARTILAGE AGGRECAN. BIOCHEMICAL JOURNAL. 1994;304:347-351.
FOSANG, A. J., LAST, K., NEAME, P. J., MURPHY, G., KNAUPER, V., Tschesche, H., HUGHES, C. E., et al. (1994). NEUTROPHIL COLLAGENASE (MMP-8) CLEAVES AT THE AGGRECANASE SITE E(373)-A(374) IN THE INTERGLOBULAR DOMAIN OF CARTILAGE AGGRECAN. BIOCHEMICAL JOURNAL, 304, 347-351.
FOSANG, A. J., LAST, K., NEAME, P. J., MURPHY, G., KNAUPER, V., Tschesche, H., HUGHES, C. E., CATERSON, B., and HARDINGHAM, T. E. (1994). NEUTROPHIL COLLAGENASE (MMP-8) CLEAVES AT THE AGGRECANASE SITE E(373)-A(374) IN THE INTERGLOBULAR DOMAIN OF CARTILAGE AGGRECAN. BIOCHEMICAL JOURNAL 304, 347-351.
FOSANG, A.J., et al., 1994. NEUTROPHIL COLLAGENASE (MMP-8) CLEAVES AT THE AGGRECANASE SITE E(373)-A(374) IN THE INTERGLOBULAR DOMAIN OF CARTILAGE AGGRECAN. BIOCHEMICAL JOURNAL, 304, p 347-351.
A.J. FOSANG, et al., “NEUTROPHIL COLLAGENASE (MMP-8) CLEAVES AT THE AGGRECANASE SITE E(373)-A(374) IN THE INTERGLOBULAR DOMAIN OF CARTILAGE AGGRECAN”, BIOCHEMICAL JOURNAL, vol. 304, 1994, pp. 347-351.
FOSANG, A.J., LAST, K., NEAME, P.J., MURPHY, G., KNAUPER, V., Tschesche, H., HUGHES, C.E., CATERSON, B., HARDINGHAM, T.E.: NEUTROPHIL COLLAGENASE (MMP-8) CLEAVES AT THE AGGRECANASE SITE E(373)-A(374) IN THE INTERGLOBULAR DOMAIN OF CARTILAGE AGGRECAN. BIOCHEMICAL JOURNAL. 304, 347-351 (1994).
FOSANG, AJ, LAST, K, NEAME, PJ, MURPHY, G, KNAUPER, V, Tschesche, Harald, HUGHES, CE, CATERSON, B, and HARDINGHAM, TE. “NEUTROPHIL COLLAGENASE (MMP-8) CLEAVES AT THE AGGRECANASE SITE E(373)-A(374) IN THE INTERGLOBULAR DOMAIN OF CARTILAGE AGGRECAN”. BIOCHEMICAL JOURNAL 304 (1994): 347-351.
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