NEUTROPHIL COLLAGENASE (MMP-8) CLEAVES AT THE AGGRECANASE SITE E(373)-A(374) IN THE INTERGLOBULAR DOMAIN OF CARTILAGE AGGRECAN

FOSANG AJ, LAST K, NEAME PJ, MURPHY G, KNAUPER V, Tschesche H, HUGHES CE, CATERSON B, HARDINGHAM TE (1994)
BIOCHEMICAL JOURNAL 304: 347-351.

Download
No fulltext has been uploaded. References only!
Journal Article | Original Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ; ; ; ;
Abstract
Native and recombinant neutrophil collagenase (MMP-8) was shown to cleave at the E(373)-A(374) 'aggrecanase'' site in the interglobular domain of aggrecan. The time course of digestion in vitro showed that MMP-8 cleaved initially at N-341-F-342, the predominant metalloproteinase site, before cleaving at the E(373)-A(374) site. A synthetic peptide, IPENFFG, inhibited cleavage at E(373)-A(374) but not N-341-F-342 in vitro, indicating that the E(373)-A(374) sequence was a less preferred site for MMP-8 cleavage than N-341-F-342. IPENFFG also inhibited release of A(374) RGSVI fragments from cartilage in explant culture, suggesting that a metalloproteinase cleaved at the aggrecanase site in situ. The possibility remains that 'aggrecanase' may be a metalloproteinase in cartilage.
Publishing Year
ISSN
PUB-ID

Cite this

FOSANG AJ, LAST K, NEAME PJ, et al. NEUTROPHIL COLLAGENASE (MMP-8) CLEAVES AT THE AGGRECANASE SITE E(373)-A(374) IN THE INTERGLOBULAR DOMAIN OF CARTILAGE AGGRECAN. BIOCHEMICAL JOURNAL. 1994;304:347-351.
FOSANG, A. J., LAST, K., NEAME, P. J., MURPHY, G., KNAUPER, V., Tschesche, H., HUGHES, C. E., et al. (1994). NEUTROPHIL COLLAGENASE (MMP-8) CLEAVES AT THE AGGRECANASE SITE E(373)-A(374) IN THE INTERGLOBULAR DOMAIN OF CARTILAGE AGGRECAN. BIOCHEMICAL JOURNAL, 304, 347-351.
FOSANG, A. J., LAST, K., NEAME, P. J., MURPHY, G., KNAUPER, V., Tschesche, H., HUGHES, C. E., CATERSON, B., and HARDINGHAM, T. E. (1994). NEUTROPHIL COLLAGENASE (MMP-8) CLEAVES AT THE AGGRECANASE SITE E(373)-A(374) IN THE INTERGLOBULAR DOMAIN OF CARTILAGE AGGRECAN. BIOCHEMICAL JOURNAL 304, 347-351.
FOSANG, A.J., et al., 1994. NEUTROPHIL COLLAGENASE (MMP-8) CLEAVES AT THE AGGRECANASE SITE E(373)-A(374) IN THE INTERGLOBULAR DOMAIN OF CARTILAGE AGGRECAN. BIOCHEMICAL JOURNAL, 304, p 347-351.
A.J. FOSANG, et al., “NEUTROPHIL COLLAGENASE (MMP-8) CLEAVES AT THE AGGRECANASE SITE E(373)-A(374) IN THE INTERGLOBULAR DOMAIN OF CARTILAGE AGGRECAN”, BIOCHEMICAL JOURNAL, vol. 304, 1994, pp. 347-351.
FOSANG, A.J., LAST, K., NEAME, P.J., MURPHY, G., KNAUPER, V., Tschesche, H., HUGHES, C.E., CATERSON, B., HARDINGHAM, T.E.: NEUTROPHIL COLLAGENASE (MMP-8) CLEAVES AT THE AGGRECANASE SITE E(373)-A(374) IN THE INTERGLOBULAR DOMAIN OF CARTILAGE AGGRECAN. BIOCHEMICAL JOURNAL. 304, 347-351 (1994).
FOSANG, AJ, LAST, K, NEAME, PJ, MURPHY, G, KNAUPER, V, Tschesche, Harald, HUGHES, CE, CATERSON, B, and HARDINGHAM, TE. “NEUTROPHIL COLLAGENASE (MMP-8) CLEAVES AT THE AGGRECANASE SITE E(373)-A(374) IN THE INTERGLOBULAR DOMAIN OF CARTILAGE AGGRECAN”. BIOCHEMICAL JOURNAL 304 (1994): 347-351.
This data publication is cited in the following publications:
This publication cites the following data publications:

56 Citations in Europe PMC

Data provided by Europe PubMed Central.

In vitro response of human chondrocytes to a combination of growth factors and a proteinase inhibitor.
Pifer MA, Kibuule LK, Maerz T, Studzinski DM, Baker KC, Herkowitz HN., Orthopedics 35(1), 2012
PMID: 22229919
A quantitative evaluation of inflammatory cells in human temporomandibular joint tissues from patients with and without implants.
Alonso A, Kaimal S, Look J, Swift J, Fricton J, Myers S, Kehl L., J. Oral Maxillofac. Surg. 67(4), 2009
PMID: 19304036
Characterization of an ADAMTS-5-mediated cleavage site in aggrecan in OSM-stimulated bovine cartilage.
Durigova M, Soucy P, Fushimi K, Nagase H, Mort JS, Roughley PJ., Osteoarthr. Cartil. 16(10), 2008
PMID: 18372197
Physiological and behavioral evidence for focal nociception induced by epidural glutamate infusion in rats.
Harrington JF, Messier AA, Hoffman L, Yu E, Dykhuizen M, Barker K., Spine 30(6), 2005
PMID: 15770173
Stromelysin 1, neutrophil collagenase, and collagenase 3 do not play major roles in a model of chondrocyte mediated cartilage breakdown.
Kozaci LD, Brown CJ, Adcocks C, Galloway A, Hollander AP, Buttle DJ., MP, Mol. Pathol. 51(5), 1998
PMID: 10193524
Aggrecan: A Target Molecule of Autoimmune Reactions.
Buzas EI, Mikecz K, Glant TT., Pathol. Oncol. Res. 2(4), 1996
PMID: 11173607

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 7998967
PubMed | Europe PMC

Search this title in

Google Scholar