NEUTROPHIL COLLAGENASE (MMP-8) CLEAVES AT THE AGGRECANASE SITE E(373)-A(374) IN THE INTERGLOBULAR DOMAIN OF CARTILAGE AGGRECAN

FOSANG AJ, LAST K, NEAME PJ, MURPHY G, KNAUPER V, Tschesche H, HUGHES CE, CATERSON B, HARDINGHAM TE (1994)
BIOCHEMICAL JOURNAL 304: 347-351.

Download
No fulltext has been uploaded. References only!
Journal Article | Original Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ; ; ; ;
Abstract
Native and recombinant neutrophil collagenase (MMP-8) was shown to cleave at the E(373)-A(374) 'aggrecanase'' site in the interglobular domain of aggrecan. The time course of digestion in vitro showed that MMP-8 cleaved initially at N-341-F-342, the predominant metalloproteinase site, before cleaving at the E(373)-A(374) site. A synthetic peptide, IPENFFG, inhibited cleavage at E(373)-A(374) but not N-341-F-342 in vitro, indicating that the E(373)-A(374) sequence was a less preferred site for MMP-8 cleavage than N-341-F-342. IPENFFG also inhibited release of A(374) RGSVI fragments from cartilage in explant culture, suggesting that a metalloproteinase cleaved at the aggrecanase site in situ. The possibility remains that 'aggrecanase' may be a metalloproteinase in cartilage.
Publishing Year
ISSN
PUB-ID

Cite this

FOSANG AJ, LAST K, NEAME PJ, et al. NEUTROPHIL COLLAGENASE (MMP-8) CLEAVES AT THE AGGRECANASE SITE E(373)-A(374) IN THE INTERGLOBULAR DOMAIN OF CARTILAGE AGGRECAN. BIOCHEMICAL JOURNAL. 1994;304:347-351.
FOSANG, A. J., LAST, K., NEAME, P. J., MURPHY, G., KNAUPER, V., Tschesche, H., HUGHES, C. E., et al. (1994). NEUTROPHIL COLLAGENASE (MMP-8) CLEAVES AT THE AGGRECANASE SITE E(373)-A(374) IN THE INTERGLOBULAR DOMAIN OF CARTILAGE AGGRECAN. BIOCHEMICAL JOURNAL, 304, 347-351.
FOSANG, A. J., LAST, K., NEAME, P. J., MURPHY, G., KNAUPER, V., Tschesche, H., HUGHES, C. E., CATERSON, B., and HARDINGHAM, T. E. (1994). NEUTROPHIL COLLAGENASE (MMP-8) CLEAVES AT THE AGGRECANASE SITE E(373)-A(374) IN THE INTERGLOBULAR DOMAIN OF CARTILAGE AGGRECAN. BIOCHEMICAL JOURNAL 304, 347-351.
FOSANG, A.J., et al., 1994. NEUTROPHIL COLLAGENASE (MMP-8) CLEAVES AT THE AGGRECANASE SITE E(373)-A(374) IN THE INTERGLOBULAR DOMAIN OF CARTILAGE AGGRECAN. BIOCHEMICAL JOURNAL, 304, p 347-351.
A.J. FOSANG, et al., “NEUTROPHIL COLLAGENASE (MMP-8) CLEAVES AT THE AGGRECANASE SITE E(373)-A(374) IN THE INTERGLOBULAR DOMAIN OF CARTILAGE AGGRECAN”, BIOCHEMICAL JOURNAL, vol. 304, 1994, pp. 347-351.
FOSANG, A.J., LAST, K., NEAME, P.J., MURPHY, G., KNAUPER, V., Tschesche, H., HUGHES, C.E., CATERSON, B., HARDINGHAM, T.E.: NEUTROPHIL COLLAGENASE (MMP-8) CLEAVES AT THE AGGRECANASE SITE E(373)-A(374) IN THE INTERGLOBULAR DOMAIN OF CARTILAGE AGGRECAN. BIOCHEMICAL JOURNAL. 304, 347-351 (1994).
FOSANG, AJ, LAST, K, NEAME, PJ, MURPHY, G, KNAUPER, V, Tschesche, Harald, HUGHES, CE, CATERSON, B, and HARDINGHAM, TE. “NEUTROPHIL COLLAGENASE (MMP-8) CLEAVES AT THE AGGRECANASE SITE E(373)-A(374) IN THE INTERGLOBULAR DOMAIN OF CARTILAGE AGGRECAN”. BIOCHEMICAL JOURNAL 304 (1994): 347-351.
This data publication is cited in the following publications:
This publication cites the following data publications:

56 Citations in Europe PMC

Data provided by Europe PubMed Central.

Activation of human neutrophil procollagenase by stromelysin 2.
Knäuper V, Murphy G, Tschesche H., Eur J Biochem 235(1-2), 1996
PMID: 8631328
Collagenase: a key enzyme in collagen turnover.
Shingleton WD, Hodges DJ, Brick P, Cawston TE., Biochem Cell Biol 74(6), 1996
PMID: 9164646
Proteolytic mechanisms of cartilage breakdown: a target for arthritis therapy?
Buttle DJ, Bramwell H, Hollander AP., Clin Mol Pathol 48(4), 1995
PMID: 16696000

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 7998967
PubMed | Europe PMC

Search this title in

Google Scholar