CLONING AND CHARACTERIZATION OF THE HUMAN GENE ENCODING ASPARTYL BETA-HYDROXYLASE

KORIOTH F, GIEFFERS C, Frey J (1994)
GENE 150(2): 395-399.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ;
Abstract
Sequence information for aspartyl beta-hydroxylase (AspH), which specifically hydroxylates one Asp or Asn residue in certain epidermal growth factor (EGF)-like domains oi a number of proteins, is so far only described for bovine species. We have isolated a 4.3-kb cDNA encoding the human AspH (hAspH) by immunoscreening of a human osteosarcoma (MG63) cDNA library in lambda ZAP with an antiserum raised against membrane fractions of these cells. Northern blot analyses revealed two transcripts with lengths of 2.6 and 4.3 kb. The deduced amino acid (aa) sequence of this cDNA encodes a protein of 757 aa (85 kDa). Comparison with the deduced bovine AspH (bAspH) aa sequence showed striking differences in the N-terminal portion of this protein. In vitro transcription and translation in the presence of canine pancreas microsomes yielded a 56-kDa protein. Western blot analyses of membrane fractions from MG63 cells with AspH-specific antibodies revealed a protein of the same M(r). These results suggest a posttranslational cleavage of the catalytic C terminus in the lumen of the endoplasmic reticulum.
Publishing Year
ISSN
PUB-ID

Cite this

KORIOTH F, GIEFFERS C, Frey J. CLONING AND CHARACTERIZATION OF THE HUMAN GENE ENCODING ASPARTYL BETA-HYDROXYLASE. GENE. 1994;150(2):395-399.
KORIOTH, F., GIEFFERS, C., & Frey, J. (1994). CLONING AND CHARACTERIZATION OF THE HUMAN GENE ENCODING ASPARTYL BETA-HYDROXYLASE. GENE, 150(2), 395-399.
KORIOTH, F., GIEFFERS, C., and Frey, J. (1994). CLONING AND CHARACTERIZATION OF THE HUMAN GENE ENCODING ASPARTYL BETA-HYDROXYLASE. GENE 150, 395-399.
KORIOTH, F., GIEFFERS, C., & Frey, J., 1994. CLONING AND CHARACTERIZATION OF THE HUMAN GENE ENCODING ASPARTYL BETA-HYDROXYLASE. GENE, 150(2), p 395-399.
F. KORIOTH, C. GIEFFERS, and J. Frey, “CLONING AND CHARACTERIZATION OF THE HUMAN GENE ENCODING ASPARTYL BETA-HYDROXYLASE”, GENE, vol. 150, 1994, pp. 395-399.
KORIOTH, F., GIEFFERS, C., Frey, J.: CLONING AND CHARACTERIZATION OF THE HUMAN GENE ENCODING ASPARTYL BETA-HYDROXYLASE. GENE. 150, 395-399 (1994).
KORIOTH, F, GIEFFERS, C, and Frey, Jürgen. “CLONING AND CHARACTERIZATION OF THE HUMAN GENE ENCODING ASPARTYL BETA-HYDROXYLASE”. GENE 150.2 (1994): 395-399.
This data publication is cited in the following publications:
This publication cites the following data publications:

17 Citations in Europe PMC

Data provided by Europe PubMed Central.

Overexpression of aspartyl-(asparaginyl)-beta-hydroxylase in hepatocellular carcinoma is associated with worse surgical outcome.
Wang K, Liu J, Yan ZL, Li J, Shi LH, Cong WM, Xia Y, Zou QF, Xi T, Shen F, Wang HY, Wu MC., Hepatology 52(1), 2010
PMID: 20578260
Origin and evolution of peptide-modifying dioxygenases and identification of the wybutosine hydroxylase/hydroperoxidase.
Iyer LM, Abhiman S, de Souza RF, Aravind L., Nucleic Acids Res. 38(16), 2010
PMID: 20423905
Prognostic value of aspartyl (asparaginyl)-beta-hydroxylase/humbug expression in non-small cell lung carcinoma.
Luu M, Sabo E, de la Monte SM, Greaves W, Wang J, Tavares R, Simao L, Wands JR, Resnick MB, Wang L., Hum. Pathol. 40(5), 2009
PMID: 19200576
Multiple functions of junctin and junctate, two distinct isoforms of aspartyl beta-hydroxylase.
Hong CS, Kwon SJ, Kim DH., Biochem. Biophys. Res. Commun. 362(1), 2007
PMID: 17706594
Prognostic value of humbug gene overexpression in stage II colon cancer.
Wang J, de la Monte SM, Sabo E, Kethu S, Tavares R, Branda M, Simao L, Wands JR, Resnick MB., Hum. Pathol. 38(1), 2007
PMID: 17020779
Aspartyl-asparagyl beta hydroxylase over-expression in human hepatoma is linked to activation of insulin-like growth factor and notch signaling mechanisms.
Cantarini MC, de la Monte SM, Pang M, Tong M, D'Errico A, Trevisani F, Wands JR., Hepatology 44(2), 2006
PMID: 16871543
cDNA cloning and characterization of human cardiac junctin.
Lim KY, Hong CS, Kim DH., Gene 255(1), 2000
PMID: 10974562
Molecular cloning of junctin from human and developing rabbit heart.
Wetzel GT, Ding S, Chen F., Mol. Genet. Metab. 69(3), 2000
PMID: 10767180
Immunological approach to hepatocellular carcinoma.
Wands JR, Lavaissiere L, Moradpour D, de la Monte S, Mohr L, Nicolau C, Tanaka S., J. Viral Hepat. 4 Suppl 2(), 1997
PMID: 9429211
Overexpression of human aspartyl(asparaginyl)beta-hydroxylase in hepatocellular carcinoma and cholangiocarcinoma.
Lavaissiere L, Jia S, Nishiyama M, de la Monte S, Stern AM, Wands JR, Friedman PA., J. Clin. Invest. 98(6), 1996
PMID: 8823296

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 7821814
PubMed | Europe PMC

Search this title in

Google Scholar