Isolation of insertion elements from gram-positive Brevibacterium, Corynebacterium and Rhodococcus strains using the Bacillus subtilis sacB gene as a positive selection marker

Jäger W, Schäfer A, Kalinowski J, Pühler A (1995)
FEMS MICROBIOLOGY LETTERS 126(1): 1-6.

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The sacB gene of Bacillus subtilis was successfully applied in various Arthrobacter, Brevibacterium, Corynebacterium and Rhodococcus strains for the isolation of transposable elements. Three different insertion sequence (IS) elements entrapped in sacB were isolated. The IS elements IS-BI and IS-Cg isolated from Brevibacterium lactofermentum and Corynebacterium glutamicum, respectively, were found to be similar in size (1.45 kb) and generated target duplications of 8 bp. Their inverted repeats showed homology. In contrast, the IS element IS-Rf isolated from Rhodococcus fascians was only 1.3 kb long and generated a 3-bp target duplication. IS-Cg and IS-Rf were not restricted to their original host strains, and we also found strains harbouring more than one element.
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Jäger W, Schäfer A, Kalinowski J, Pühler A. Isolation of insertion elements from gram-positive Brevibacterium, Corynebacterium and Rhodococcus strains using the Bacillus subtilis sacB gene as a positive selection marker. FEMS MICROBIOLOGY LETTERS. 1995;126(1):1-6.
Jäger, W., Schäfer, A., Kalinowski, J., & Pühler, A. (1995). Isolation of insertion elements from gram-positive Brevibacterium, Corynebacterium and Rhodococcus strains using the Bacillus subtilis sacB gene as a positive selection marker. FEMS MICROBIOLOGY LETTERS, 126(1), 1-6.
Jäger, W., Schäfer, A., Kalinowski, J., and Pühler, A. (1995). Isolation of insertion elements from gram-positive Brevibacterium, Corynebacterium and Rhodococcus strains using the Bacillus subtilis sacB gene as a positive selection marker. FEMS MICROBIOLOGY LETTERS 126, 1-6.
Jäger, W., et al., 1995. Isolation of insertion elements from gram-positive Brevibacterium, Corynebacterium and Rhodococcus strains using the Bacillus subtilis sacB gene as a positive selection marker. FEMS MICROBIOLOGY LETTERS, 126(1), p 1-6.
W. Jäger, et al., “Isolation of insertion elements from gram-positive Brevibacterium, Corynebacterium and Rhodococcus strains using the Bacillus subtilis sacB gene as a positive selection marker”, FEMS MICROBIOLOGY LETTERS, vol. 126, 1995, pp. 1-6.
Jäger, W., Schäfer, A., Kalinowski, J., Pühler, A.: Isolation of insertion elements from gram-positive Brevibacterium, Corynebacterium and Rhodococcus strains using the Bacillus subtilis sacB gene as a positive selection marker. FEMS MICROBIOLOGY LETTERS. 126, 1-6 (1995).
Jäger, W, Schäfer, A, Kalinowski, Jörn, and Pühler, Alfred. “Isolation of insertion elements from gram-positive Brevibacterium, Corynebacterium and Rhodococcus strains using the Bacillus subtilis sacB gene as a positive selection marker”. FEMS MICROBIOLOGY LETTERS 126.1 (1995): 1-6.
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Precision-engineering the Pseudomonas aeruginosa genome with two-step allelic exchange.
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