MOLECULAR ANALYSIS OF THE RHIZOBIUM-MELILOTI MUCR GENE REGULATING THE BIOSYNTHESIS OF THE EXOPOLYSACCHARIDES SUCCINOGLYCAN AND GALACTOGLUCAN

KELLER M, ROXLAU A, WENG WM, SCHMIDT M, QUANDT J, Niehaus K, Jording D, Arnold W, Pühler A (1995)
MOLECULAR PLANT-MICROBE INTERACTIONS 8(2): 267-277.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ; ; ; ;
Abstract
The Rhizobium meliloti Tn5 mutant Rm3131, producing galactoglucan (EPS II) instead of succinoglycan (EPS I), was complemented by a 3.6-kb EcoRI-fragment of the Rhizobium meliloti genome. Sequencing of this fragment revealed six open reading frames (ORFs). The ORF found to be affected in the mutant Rm3131 codes for a putative protein of 15.7 kDa and forms a monocistronic transcriptional unit. Further genetic analysis revealed that the gene mutated in Rm3131 is identical to the previously described R. meliloti mucR gene (H. Zhan, S. B. Levery, C. C. Lee, and J. A. Leigh, 1989, Proc. Natl. Acad. Sci. USA 86:3055-3059). By hybridization it was shown that a mucR homologous gene is present in several rhizobacteria. The deduced amino acid sequence of MucR showed nearly 80% identity to the Agrobacterium tumefaciens Ros protein, a negative regulator of vir genes and necessary for succinoglycan production. MucR contains like Ros a putative zinc finger sequence of the C2H2 type. Transcriptional fusions of genes for EPS I and EPS II synthesis, the so-called exo and exp genes, with the marker gene lacZ were used to delineate the role of mucR for exo and exp gene expression. It was found that exp genes are negatively regulated by MucR on the transcriptional level, whereas a posttranscriptional regulation by MucR is assumed for exo genes. Furthermore, mucR is negatively regulating its own transcription.
Publishing Year
ISSN
PUB-ID

Cite this

KELLER M, ROXLAU A, WENG WM, et al. MOLECULAR ANALYSIS OF THE RHIZOBIUM-MELILOTI MUCR GENE REGULATING THE BIOSYNTHESIS OF THE EXOPOLYSACCHARIDES SUCCINOGLYCAN AND GALACTOGLUCAN. MOLECULAR PLANT-MICROBE INTERACTIONS. 1995;8(2):267-277.
KELLER, M., ROXLAU, A., WENG, W. M., SCHMIDT, M., QUANDT, J., Niehaus, K., Jording, D., et al. (1995). MOLECULAR ANALYSIS OF THE RHIZOBIUM-MELILOTI MUCR GENE REGULATING THE BIOSYNTHESIS OF THE EXOPOLYSACCHARIDES SUCCINOGLYCAN AND GALACTOGLUCAN. MOLECULAR PLANT-MICROBE INTERACTIONS, 8(2), 267-277.
KELLER, M., ROXLAU, A., WENG, W. M., SCHMIDT, M., QUANDT, J., Niehaus, K., Jording, D., Arnold, W., and Pühler, A. (1995). MOLECULAR ANALYSIS OF THE RHIZOBIUM-MELILOTI MUCR GENE REGULATING THE BIOSYNTHESIS OF THE EXOPOLYSACCHARIDES SUCCINOGLYCAN AND GALACTOGLUCAN. MOLECULAR PLANT-MICROBE INTERACTIONS 8, 267-277.
KELLER, M., et al., 1995. MOLECULAR ANALYSIS OF THE RHIZOBIUM-MELILOTI MUCR GENE REGULATING THE BIOSYNTHESIS OF THE EXOPOLYSACCHARIDES SUCCINOGLYCAN AND GALACTOGLUCAN. MOLECULAR PLANT-MICROBE INTERACTIONS, 8(2), p 267-277.
M. KELLER, et al., “MOLECULAR ANALYSIS OF THE RHIZOBIUM-MELILOTI MUCR GENE REGULATING THE BIOSYNTHESIS OF THE EXOPOLYSACCHARIDES SUCCINOGLYCAN AND GALACTOGLUCAN”, MOLECULAR PLANT-MICROBE INTERACTIONS, vol. 8, 1995, pp. 267-277.
KELLER, M., ROXLAU, A., WENG, W.M., SCHMIDT, M., QUANDT, J., Niehaus, K., Jording, D., Arnold, W., Pühler, A.: MOLECULAR ANALYSIS OF THE RHIZOBIUM-MELILOTI MUCR GENE REGULATING THE BIOSYNTHESIS OF THE EXOPOLYSACCHARIDES SUCCINOGLYCAN AND GALACTOGLUCAN. MOLECULAR PLANT-MICROBE INTERACTIONS. 8, 267-277 (1995).
KELLER, M, ROXLAU, A, WENG, WM, SCHMIDT, M, QUANDT, J, Niehaus, Karsten, Jording, Doris, Arnold, Walter, and Pühler, Alfred. “MOLECULAR ANALYSIS OF THE RHIZOBIUM-MELILOTI MUCR GENE REGULATING THE BIOSYNTHESIS OF THE EXOPOLYSACCHARIDES SUCCINOGLYCAN AND GALACTOGLUCAN”. MOLECULAR PLANT-MICROBE INTERACTIONS 8.2 (1995): 267-277.
This data publication is cited in the following publications:
This publication cites the following data publications:

39 Citations in Europe PMC

Data provided by Europe PubMed Central.

Bacterial exopolysaccharides: biosynthesis pathways and engineering strategies.
Schmid J, Sieber V, Rehm B., Front Microbiol 6(), 2015
PMID: 26074894
The Sinorhizobium meliloti ntrX gene is involved in succinoglycan production, motility, and symbiotic nodulation on alfalfa.
Wang D, Xue H, Wang Y, Yin R, Xie F, Luo L., Appl. Environ. Microbiol. 79(23), 2013
PMID: 24038694
Diverse genetic regulon of the virulence-associated transcriptional regulator MucR in Brucella abortus 2308.
Caswell CC, Elhassanny AE, Planchin EE, Roux CM, Weeks-Gorospe JN, Ficht TA, Dunman PM, Roop RM 2nd., Infect. Immun. 81(4), 2013
PMID: 23319565
A positive correlation between bacterial autoaggregation and biofilm formation in native Sinorhizobium meliloti isolates from Argentina.
Sorroche FG, Spesia MB, Zorreguieta A, Giordano W., Appl. Environ. Microbiol. 78(12), 2012
PMID: 22492433
Insights from the architecture of the bacterial transcription apparatus.
Iyer LM, Aravind L., J. Struct. Biol. 179(3), 2012
PMID: 22210308
Functional genomics of dichloromethane utilization in Methylobacterium extorquens DM4.
Muller EE, Hourcade E, Louhichi-Jelail Y, Hammann P, Vuilleumier S, Bringel F., Environ. Microbiol. 13(9), 2011
PMID: 21854516
PCR use of highly conserved DNA regions for identification of Sinorhizobium meliloti.
Sanchez-Contreras M, Lloret J, Martin M, Villacieros M, Bonilla I, Rivilla R., Appl. Environ. Microbiol. 66(8), 2000
PMID: 10919829

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 7756693
PubMed | Europe PMC

Search this title in

Google Scholar