CONSTRUCTION AND CHARACTERIZATION OF A RHIZOBIUM-LEGUMINOSARUM BIOVAR VICIAE STRAIN DESIGNED TO ASSESS HORIZONTAL GENE-TRANSFER IN THE ENVIRONMENT

Selbitschka W, Jording D, NIEMAN S, SCHMIDT R, Pühler A, MENDUM T, HIRSCH P (1995)
FEMS MICROBIOLOGY LETTERS 128(3): 255-263.

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An integration vector was developed which inserts cloned DNA in a non-essential site of the Rhizobium leguminosarum biovar viciae chromosome. The expression of integrated genes is under the control of the constitutive neomycin phosphotransferase II (nptII) promotor of transposon Tn5. The design of the vector ensures that loss of vector sequences can be detected, enabling selection of progeny containing only the requisite DNA. The newly constructed vector was employed to insert the Escherichia coli gusA gene conferring GUS activity into R. leguminosarum by. viciae strain LRS39401 which is cured of its symbiotic plasmid (pSym). One GUS-positive transconjugant, strain CT0370, was shown to have lost all vector sequences. Conjugal transfer of pSym2004 (a Tn5-tagged derivative of symbiotic plasmid pRL1JI, which specifies pea nodulation and symbiotic nitrogen fixation) to CT0370, restored the GUS-positive strain's symbiotic proficiency. Strain CT0370 is presently being used in a field release experiment in order to assess the extent of pSym transfer in a natural R. leguminosarum by. viciae population under environmental conditions.
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Selbitschka W, Jording D, NIEMAN S, et al. CONSTRUCTION AND CHARACTERIZATION OF A RHIZOBIUM-LEGUMINOSARUM BIOVAR VICIAE STRAIN DESIGNED TO ASSESS HORIZONTAL GENE-TRANSFER IN THE ENVIRONMENT. FEMS MICROBIOLOGY LETTERS. 1995;128(3):255-263.
Selbitschka, W., Jording, D., NIEMAN, S., SCHMIDT, R., Pühler, A., MENDUM, T., & HIRSCH, P. (1995). CONSTRUCTION AND CHARACTERIZATION OF A RHIZOBIUM-LEGUMINOSARUM BIOVAR VICIAE STRAIN DESIGNED TO ASSESS HORIZONTAL GENE-TRANSFER IN THE ENVIRONMENT. FEMS MICROBIOLOGY LETTERS, 128(3), 255-263.
Selbitschka, W., Jording, D., NIEMAN, S., SCHMIDT, R., Pühler, A., MENDUM, T., and HIRSCH, P. (1995). CONSTRUCTION AND CHARACTERIZATION OF A RHIZOBIUM-LEGUMINOSARUM BIOVAR VICIAE STRAIN DESIGNED TO ASSESS HORIZONTAL GENE-TRANSFER IN THE ENVIRONMENT. FEMS MICROBIOLOGY LETTERS 128, 255-263.
Selbitschka, W., et al., 1995. CONSTRUCTION AND CHARACTERIZATION OF A RHIZOBIUM-LEGUMINOSARUM BIOVAR VICIAE STRAIN DESIGNED TO ASSESS HORIZONTAL GENE-TRANSFER IN THE ENVIRONMENT. FEMS MICROBIOLOGY LETTERS, 128(3), p 255-263.
W. Selbitschka, et al., “CONSTRUCTION AND CHARACTERIZATION OF A RHIZOBIUM-LEGUMINOSARUM BIOVAR VICIAE STRAIN DESIGNED TO ASSESS HORIZONTAL GENE-TRANSFER IN THE ENVIRONMENT”, FEMS MICROBIOLOGY LETTERS, vol. 128, 1995, pp. 255-263.
Selbitschka, W., Jording, D., NIEMAN, S., SCHMIDT, R., Pühler, A., MENDUM, T., HIRSCH, P.: CONSTRUCTION AND CHARACTERIZATION OF A RHIZOBIUM-LEGUMINOSARUM BIOVAR VICIAE STRAIN DESIGNED TO ASSESS HORIZONTAL GENE-TRANSFER IN THE ENVIRONMENT. FEMS MICROBIOLOGY LETTERS. 128, 255-263 (1995).
Selbitschka, Werner, Jording, Doris, NIEMAN, S, SCHMIDT, R, Pühler, Alfred, MENDUM, T, and HIRSCH, P. “CONSTRUCTION AND CHARACTERIZATION OF A RHIZOBIUM-LEGUMINOSARUM BIOVAR VICIAE STRAIN DESIGNED TO ASSESS HORIZONTAL GENE-TRANSFER IN THE ENVIRONMENT”. FEMS MICROBIOLOGY LETTERS 128.3 (1995): 255-263.
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3 Citations in Europe PMC

Data provided by Europe PubMed Central.

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Ramos HJO, Yates MG, Pedrosa FO, Souza EM., Soil Biol. Biochem. 43(8), 2011
PMID: IND44599181
Genetically modified bacteria in agriculture.
Amarger N., Biochimie 84(11), 2002
PMID: 12595134
Gene transfer in soil systems using microcosms
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PMID: IND21814441

25 References

Data provided by Europe PubMed Central.

Plasmids of Rhizobium and their role in symbiotic nitrogen fixation
Prakash, Int. Rev. Cytol. 104(), 1986
Evidence for genetic exchange and recombination of Rhizobium symbiotic plasmids in a soil population.
Schofield PR, Gibson AH, Dudman WF, Watson JM., Appl. Environ. Microbiol. 53(12), 1987
PMID: 16347509
Sym plasmid and chromosomal genotypes are correlated in field populations of Rhizobium leguminosarum
Young, J. Gen. Microbiol. 134(), 1988
The planned introduction of genetically engineered organisms: ecological considerations and recommendations
Tiedje, Ecology 70(), 1989
Transfer of the Pea Symbiotic Plasmid pJB5JI in Nonsterile Soil.
Kinkle BK, Schmidt EL., Appl. Environ. Microbiol. 57(11), 1991
PMID: 16348589
R factor transfer in Rhizobium leguminosarum.
Beringer JE., J. Gen. Microbiol. 84(1), 1974
PMID: 4612098

Vincent, 1970
A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram-negative bacteria
Simon, Bio/Technology 1(), 1983

Hirsch, 1992

AUTHOR UNKNOWN, 0

Maniatis, 1982

AUTHOR UNKNOWN, 0
The construction of recA-deficient Rhizobium meliloti and R. leguminosarum strains marked with gusA or lue cassettes for use in risk-assessment studies
Selbitschka, Mol. Ecol. 1(), 1992
A biological containment system for Rhizobium meliloti based on the use of recombination-deficient (recA-) strains.
Selbitschka W, Dresing U, Hagen M, Niemann S, Puhler A., FEMS Microbiol. Ecol. 16(3), 1995
PMID: IND20456986
Characterization of recA genes and recA mutants of Rhizobium meliloti and Rhizobium leguminosarum biovar viciae.
Selbitschka W, Arnold W, Priefer UB, Rottschafer T, Schmidt M, Simon R, Puhler A., Mol. Gen. Genet. 229(1), 1991
PMID: 1896024

Döhler, 1988

Amarger, 1990
Diversity and stability of plasmid transfer in isolates from a single field population of Rhizobium leguminosarum bv
Geniaux, viciae. FEMS Microbiol. Ecol. 102(), 1993
Genetic structure of a soil population of nonsymbiotic Rhizobium leguminosarum.
Segovia L, Pinero D, Palacios R, Martinez-Romero E., Appl. Environ. Microbiol. 57(2), 1991
PMID: 1707606
Isolation from soil of symbiotic and nonsymbiotic Rhizobium leguminosarum by DNA hybridization
Laguerre, Can.J. Microbiol. 39(), 1993
A complementation analysis of the restriction and modification of DNA in Escherichia coli.
Boyer HW, Roulland-Dussoix D., J. Mol. Biol. 41(3), 1969
PMID: 4896022

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