A SANDWICH ENZYME-IMMUNOASSAY FOR THE DETERMINATION OF NEUTROPHIL LIPOCALIN IN BODY-FLUIDS

BLASER J, TRIEBEL S, Tschesche H (1995)
CLINICA CHIMICA ACTA 235(2): 137-145.

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Abstract
Human neutrophil lipocalin was purified from human buffycoat. A polyclonal antibody was obtained by immunisation of rabbits. The antibody reacted with the free lipocalin as well as with the PMNL-gelatinase bound protein. This antibody was used to establish a sensitive sandwich-ELISA for the determination of the protein in body fluids using the biotin/streptavidin system. The mean intra-assay C.V. was 2.3% and the mean inter-assay C.V. 6.7%. The recovery in human plasma was determined to be 98.8%. The ELISA allowed the determination of the protein in the concentration range 0.2-25 mu g/l. Measurement of the neutrophil lipocalin concentration showed that human plasma of healthy donors contained 97 +/- 81 mu g/l (n = 122) and that the concentrations in serum were significantly higher (P < 0,001) with 133 +/- 90 mu g/l (n = 122). Neutrophil lipocalin was also found in the urine of healthy donors (8.1 mu g/l; n = 9). Very high concentrations of this lipocalin were found in the synovial fluids of patients suffering from inflammatory rheumatoid arthritis (1.7 +/- 1.4 mg/l; n = 37).
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BLASER J, TRIEBEL S, Tschesche H. A SANDWICH ENZYME-IMMUNOASSAY FOR THE DETERMINATION OF NEUTROPHIL LIPOCALIN IN BODY-FLUIDS. CLINICA CHIMICA ACTA. 1995;235(2):137-145.
BLASER, J., TRIEBEL, S., & Tschesche, H. (1995). A SANDWICH ENZYME-IMMUNOASSAY FOR THE DETERMINATION OF NEUTROPHIL LIPOCALIN IN BODY-FLUIDS. CLINICA CHIMICA ACTA, 235(2), 137-145.
BLASER, J., TRIEBEL, S., and Tschesche, H. (1995). A SANDWICH ENZYME-IMMUNOASSAY FOR THE DETERMINATION OF NEUTROPHIL LIPOCALIN IN BODY-FLUIDS. CLINICA CHIMICA ACTA 235, 137-145.
BLASER, J., TRIEBEL, S., & Tschesche, H., 1995. A SANDWICH ENZYME-IMMUNOASSAY FOR THE DETERMINATION OF NEUTROPHIL LIPOCALIN IN BODY-FLUIDS. CLINICA CHIMICA ACTA, 235(2), p 137-145.
J. BLASER, S. TRIEBEL, and H. Tschesche, “A SANDWICH ENZYME-IMMUNOASSAY FOR THE DETERMINATION OF NEUTROPHIL LIPOCALIN IN BODY-FLUIDS”, CLINICA CHIMICA ACTA, vol. 235, 1995, pp. 137-145.
BLASER, J., TRIEBEL, S., Tschesche, H.: A SANDWICH ENZYME-IMMUNOASSAY FOR THE DETERMINATION OF NEUTROPHIL LIPOCALIN IN BODY-FLUIDS. CLINICA CHIMICA ACTA. 235, 137-145 (1995).
BLASER, J, TRIEBEL, S, and Tschesche, Harald. “A SANDWICH ENZYME-IMMUNOASSAY FOR THE DETERMINATION OF NEUTROPHIL LIPOCALIN IN BODY-FLUIDS”. CLINICA CHIMICA ACTA 235.2 (1995): 137-145.
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