EXOGENOUS GELSOLIN BINDS TO SARCOMERIC THIN-FILAMENTS WITHOUT SEVERING

GONSIOR S, Hinssen H (1995)
CELL MOTILITY AND THE CYTOSKELETON 31(3): 196-206.

Journal Article | Published | English

No fulltext has been uploaded

Author
;
Abstract
We have investigated the binding of gelsolin to thin myofilaments in situ and their stability against severing. Differentiated myotubes from chicken skeletal muscle containing cross-striated myofibrils were permeabilized with Triton X-100 and incubated with gelsolin. Immunofluorescence microscopy localized both endogenous and exogenous gelsolin in the I-Z-I-regions of the sarcomers. The staining pattern suggested a binding of the exogenous gelsolin along the entire length of the thin filaments. This binding was Ca2+ dependent, but gelsolin was not removed after subsequent addition of EGTA. The fluorescence staining for actin remained unchanged after gelsolin incubation, indicating that thin filaments in cross-striated myofibrils were resistant to the severing action of gelsolin, in contrast to the microfilaments in stress fibers. After extraction of the permeabilized cells with high ionic strength to remove tropomyosin and myosin, gelsolin still bound along the entire thin filament and the actin pattern also remained unchanged. After Triton X-100 permeabilization and high ionic strength extraction, the giant protein nebulin was found to be still present as a myofibrillar component. Gelsolin treatment after high salt extraction affected neither actin nor nebulin in the thin filaments. We therefore conclude that nebulin confers the gelsolin resistance to the sarcomeric actin filaments. (C) 1995 Wiley-Liss, Inc.
Publishing Year
ISSN
eISSN
PUB-ID

Cite this

GONSIOR S, Hinssen H. EXOGENOUS GELSOLIN BINDS TO SARCOMERIC THIN-FILAMENTS WITHOUT SEVERING. CELL MOTILITY AND THE CYTOSKELETON. 1995;31(3):196-206.
GONSIOR, S., & Hinssen, H. (1995). EXOGENOUS GELSOLIN BINDS TO SARCOMERIC THIN-FILAMENTS WITHOUT SEVERING. CELL MOTILITY AND THE CYTOSKELETON, 31(3), 196-206.
GONSIOR, S., and Hinssen, H. (1995). EXOGENOUS GELSOLIN BINDS TO SARCOMERIC THIN-FILAMENTS WITHOUT SEVERING. CELL MOTILITY AND THE CYTOSKELETON 31, 196-206.
GONSIOR, S., & Hinssen, H., 1995. EXOGENOUS GELSOLIN BINDS TO SARCOMERIC THIN-FILAMENTS WITHOUT SEVERING. CELL MOTILITY AND THE CYTOSKELETON, 31(3), p 196-206.
S. GONSIOR and H. Hinssen, “EXOGENOUS GELSOLIN BINDS TO SARCOMERIC THIN-FILAMENTS WITHOUT SEVERING”, CELL MOTILITY AND THE CYTOSKELETON, vol. 31, 1995, pp. 196-206.
GONSIOR, S., Hinssen, H.: EXOGENOUS GELSOLIN BINDS TO SARCOMERIC THIN-FILAMENTS WITHOUT SEVERING. CELL MOTILITY AND THE CYTOSKELETON. 31, 196-206 (1995).
GONSIOR, S, and Hinssen, Horst. “EXOGENOUS GELSOLIN BINDS TO SARCOMERIC THIN-FILAMENTS WITHOUT SEVERING”. CELL MOTILITY AND THE CYTOSKELETON 31.3 (1995): 196-206.
This data publication is cited in the following publications:
This publication cites the following data publications:

9 Citations in Europe PMC

Data provided by Europe PubMed Central.

The interaction of gelsolin with tropomyosin modulates actin dynamics.
Khaitlina S, Fitz H, Hinssen H., FEBS J. 280(18), 2013
PMID: 23844991
Dynamic regulation of sarcomeric actin filaments in striated muscle.
Ono S., Cytoskeleton (Hoboken) 67(11), 2010
PMID: 20737540
Electron-microscopical localization of gelsolin in various crustacean muscles.
Unger A, Hinssen H., Cell Tissue Res. 341(2), 2010
PMID: 20607291
In vivo administration of calpeptin attenuates calpain activation and cardiomyocyte loss in pressure-overloaded feline myocardium.
Mani SK, Shiraishi H, Balasubramanian S, Yamane K, Chellaiah M, Cooper G, Banik N, Zile MR, Kuppuswamy D., Am. J. Physiol. Heart Circ. Physiol. 295(1), 2008
PMID: 18487434
Characterization of lobulated fibers in limb girdle muscular dystrophy type 2A by gene expression profiling.
Keira Y, Noguchi S, Kurokawa R, Fujita M, Minami N, Hayashi YK, Kato T, Nishino I., Neurosci. Res. 57(4), 2007
PMID: 17258832
Titin-based contribution to shortening velocity of rabbit skeletal myofibrils.
Minajeva A, Neagoe C, Kulke M, Linke WA., J. Physiol. (Lond.) 540(Pt 1), 2002
PMID: 11927678
Thermolabile and calcium-dependent serum factor interferes with polymerized actin, and impairs anti-actin antibody detection.
Cancado EL, Abrantes-Lemos CP, Vilas-Boas LS, Novo NF, Carrilho FJ, Laudanna AA., J. Autoimmun. 17(3), 2001
PMID: 11712860
Distribution and orientation of rhodamine-phalloidin bound to thin filaments in skeletal and cardiac myofibrils.
Zhukarev V, Sanger JM, Sanger JW, Goldman YE, Shuman H., Cell Motil. Cytoskeleton 37(4), 1997
PMID: 9258508
Actin-titin interaction in cardiac myofibrils: probing a physiological role.
Linke WA, Ivemeyer M, Labeit S, Hinssen H, Ruegg JC, Gautel M., Biophys. J. 73(2), 1997
PMID: 9251807

45 References

Data provided by Europe PubMed Central.

Evidence that nebulin is a protein-ruler in muscle thin filaments.
Labeit S, Gibson T, Lakey A, Leonard K, Zeviani M, Knight P, Wardale J, Trinick J., FEBS Lett. 282(2), 1991
PMID: 2037050
Reversible binding of actin to gelsolin and profilin in human platelet extracts.
Lind SE, Janmey PA, Chaponnier C, Herbert TJ, Stossel TP., J. Cell Biol. 105(2), 1987
PMID: 3040771
Behaviour of connectin (titin) and nebulin in skinned muscle fibres released after extreme stretch as revealed by immunoelectron microscopy.
Maruyama K, Matsuno A, Higuchi H, Shimaoka S, Kimura S, Shimizu T., J. Muscle Res. Cell. Motil. 10(5), 1989
PMID: 2687326
Nebulin, a helical actin binding protein.
Pfuhl M, Winder SJ, Pastore A., EMBO J. 13(8), 1994
PMID: 8168478
The organization of titin (connectin) and nebulin in the sarcomeres: an immunocytolocalization study.
Pierobon-Bormioli S, Betto R, Salviati G., J. Muscle Res. Cell. Motil. 10(6), 1989
PMID: 2613884
The measurement and dynamic implications of thin filament lengths in heart muscle.
Robinson TF, Winegrad S., J. Physiol. (Lond.) 286(), 1979
PMID: 312321
Muscle gelsolin: isolation from heart tissue and characterization as an integral myofibrillar protein.
Rouayrenc JF, Fattoum A, Gabrion J, Audemard E, Kassab R., FEBS Lett. 167(1), 1984
PMID: 6321238
Differential response of stress fibers and myofibrils to gelsolin.
Sanger JM, Mittal B, Wegner A, Jockusch BM, Sanger JW., Eur. J. Cell Biol. 43(3), 1987
PMID: 3040411

Scholz, 1995
Measurement of protein using bicinchoninic acid.
Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC., Anal. Biochem. 150(1), 1985
PMID: 3843705
Understanding the functions of titin and nebulin.
Trinick J., FEBS Lett. 307(1), 1992
PMID: 1639193
Identification of an N2 line protein of striated muscle.
Wang K, Williamson CL., Proc. Natl. Acad. Sci. U.S.A. 77(6), 1980
PMID: 6997874

Wang, J. Cell Biol. 111(), 1990
Preparation and characterization of pig plasma and platelet gelsolins.
Weeds AG, Gooch J, Pope B, Harris HE., Eur. J. Biochem. 161(1), 1986
PMID: 3023087

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 7585989
PubMed | Europe PMC

Search this title in

Google Scholar