The Corynebacterium xerosis composite transposon Tn5432 consists of two identical insertion sequences, designated IS1249, flanking the erythromycin resistance gene ermCX

Tauch A, KASSING F, Kalinowski J, Pühler A (1995)
Plasmid 34(2): 119-131.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
Abstract / Bemerkung
Analysis of the 50-kb R-plasmid pTP10 from the clinical isolate Corynebacterium xerosis M82B revealed that the erythromycin resistance gene, ermCX, is located on a 4524-bp composite transposable element, Tn5432. The ends of Tn5432 are identical, direct repeats of an insertion sequence, designated IS1249, encoding a putative transposase of the IS256 family. IS1249 consists of 1385 bp with 45/42 imperfect terminal inverted repeats. The nucleotide sequence of the 1754-bp Tn5432 central region is 99% identical to the previously sequenced erythromycin resistance region of the Corynebacterium diphtheriae plasmid pNG2. It encodes the erythromycin resistance gene, ermCX, and an ORF homologous to the amino-terminal end of the transposase of IS31831 from Corynebacterium glutamicum. Transposons with regions flanking the insertion sites were recovered from the C. glutamicum chromosome by a plasmid rescue technique. Insertion of Tn5432 created 8-bp target site duplications. A Tn5432-induced isoleucine/valine-auxotrophic mutant was found to carry the transposon in the 5' region of the ilvBNC cluster; in pTP10 the transposon is inserted in a region similar to replication and partitioning functions of the Enterococcus faecalis plasmid pAD1 and the Agrobacterium tumefaciens plasmid pTAR. (C) 1995 Academic Press, Inc.
Erscheinungsjahr
Zeitschriftentitel
Plasmid
Band
34
Zeitschriftennummer
2
Seite
119-131
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PUB-ID

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Tauch A, KASSING F, Kalinowski J, Pühler A. The Corynebacterium xerosis composite transposon Tn5432 consists of two identical insertion sequences, designated IS1249, flanking the erythromycin resistance gene ermCX. Plasmid. 1995;34(2):119-131.
Tauch, A., KASSING, F., Kalinowski, J., & Pühler, A. (1995). The Corynebacterium xerosis composite transposon Tn5432 consists of two identical insertion sequences, designated IS1249, flanking the erythromycin resistance gene ermCX. Plasmid, 34(2), 119-131. doi:10.1006/plas.1995.9995
Tauch, A., KASSING, F., Kalinowski, J., and Pühler, A. (1995). The Corynebacterium xerosis composite transposon Tn5432 consists of two identical insertion sequences, designated IS1249, flanking the erythromycin resistance gene ermCX. Plasmid 34, 119-131.
Tauch, A., et al., 1995. The Corynebacterium xerosis composite transposon Tn5432 consists of two identical insertion sequences, designated IS1249, flanking the erythromycin resistance gene ermCX. Plasmid, 34(2), p 119-131.
A. Tauch, et al., “The Corynebacterium xerosis composite transposon Tn5432 consists of two identical insertion sequences, designated IS1249, flanking the erythromycin resistance gene ermCX”, Plasmid, vol. 34, 1995, pp. 119-131.
Tauch, A., KASSING, F., Kalinowski, J., Pühler, A.: The Corynebacterium xerosis composite transposon Tn5432 consists of two identical insertion sequences, designated IS1249, flanking the erythromycin resistance gene ermCX. Plasmid. 34, 119-131 (1995).
Tauch, Andreas, KASSING, F, Kalinowski, Jörn, and Pühler, Alfred. “The Corynebacterium xerosis composite transposon Tn5432 consists of two identical insertion sequences, designated IS1249, flanking the erythromycin resistance gene ermCX”. Plasmid 34.2 (1995): 119-131.

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