YAC contigs of the Rab1 and wobbler (wr) spinal muscular atrophy gene region on proximal mouse chromosome 11 and of the homologous region on human chromosome 2p

Wedemeyer N, Lengeling A, Ronsiek M, Korthaus D, Baer K, Wuttke M, Jockusch H (1996)
Genomics 32(3): 447-454.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Despite rapid progress in the physical characterization of murine and human genomes, little molecular information is available on certain regions, e.g., proximal mouse chromosome 11 (Chr 11) and human chromosome 2p (Chr 2p). We have localized the wobbler spinal atrophy gene wr to proximal mouse Chr 11, tightly linked to Rab1, a gene coding for a small GTP-binding protein, and Glns-ps1, an intronless pseudogene of the glutamine synthetase gene. We have now used these markers to construct a 1.3-Mb yeast artificial chromosome (YAC) contig of the Rab1 region on mouse Chr 11. Four YAC clones isolated from two independent YAC libraries were characterized by rare-cutting analysis, fluorescence in situ hybridization (FISH), and sequence-tagged site (STS) isolation and mapping, Rab1 and Glns-ps1 were found to be only 200 kb apart. A potential CpG island near a methylated NarI site and a trapped exon, ETG1.1, were found between these loci, and a new STS, AHY1.1, was found over 250 kb from Rab1. Two overlapping YACs were identified that contained a 150-kb region of human Chr 2p, comprising the RABI locus, AHY1.1, and the human homologue of ETG1.1, indicating a high degree of conservation of this region in the two species. We mapped AHY1.1 and thus human RABI on Chr 2p13.4-p14 using somatic cell hybrids and a radiation hybrid panel, thus extending a known region of conserved synteny between mouse Chr 11 and human Chr 2p. Recently, the gene LMGMD2B for a human recessive neuromuscular disease, limb girdle muscular dystrophy type 2B, has been mapped to 2p13-p16. The conservation between the mouse Rab1 and human RABI regions will be helpful in identifying candidate genes for the wobbler spinal muscular atrophy and in clarifying a possible relationship between zur and LMGMD2B. (C) 1996 Academic Press, Inc.
Erscheinungsjahr
Zeitschriftentitel
Genomics
Band
32
Zeitschriftennummer
3
Seite
447-454
ISSN
PUB-ID

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Wedemeyer N, Lengeling A, Ronsiek M, et al. YAC contigs of the Rab1 and wobbler (wr) spinal muscular atrophy gene region on proximal mouse chromosome 11 and of the homologous region on human chromosome 2p. Genomics. 1996;32(3):447-454.
Wedemeyer, N., Lengeling, A., Ronsiek, M., Korthaus, D., Baer, K., Wuttke, M., & Jockusch, H. (1996). YAC contigs of the Rab1 and wobbler (wr) spinal muscular atrophy gene region on proximal mouse chromosome 11 and of the homologous region on human chromosome 2p. Genomics, 32(3), 447-454. doi:10.1006/geno.1996.0140
Wedemeyer, N., Lengeling, A., Ronsiek, M., Korthaus, D., Baer, K., Wuttke, M., and Jockusch, H. (1996). YAC contigs of the Rab1 and wobbler (wr) spinal muscular atrophy gene region on proximal mouse chromosome 11 and of the homologous region on human chromosome 2p. Genomics 32, 447-454.
Wedemeyer, N., et al., 1996. YAC contigs of the Rab1 and wobbler (wr) spinal muscular atrophy gene region on proximal mouse chromosome 11 and of the homologous region on human chromosome 2p. Genomics, 32(3), p 447-454.
N. Wedemeyer, et al., “YAC contigs of the Rab1 and wobbler (wr) spinal muscular atrophy gene region on proximal mouse chromosome 11 and of the homologous region on human chromosome 2p”, Genomics, vol. 32, 1996, pp. 447-454.
Wedemeyer, N., Lengeling, A., Ronsiek, M., Korthaus, D., Baer, K., Wuttke, M., Jockusch, H.: YAC contigs of the Rab1 and wobbler (wr) spinal muscular atrophy gene region on proximal mouse chromosome 11 and of the homologous region on human chromosome 2p. Genomics. 32, 447-454 (1996).
Wedemeyer, N, Lengeling, A, Ronsiek, M, Korthaus, D, Baer, K, Wuttke, M, and Jockusch, Harald. “YAC contigs of the Rab1 and wobbler (wr) spinal muscular atrophy gene region on proximal mouse chromosome 11 and of the homologous region on human chromosome 2p”. Genomics 32.3 (1996): 447-454.

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Comparative transcription map of the wobbler critical region on mouse chromosome 11 and the homologous region on human chromosome 2p13-14.
Fuchs S, Resch K, Thiel C, Ulbrich M, Platzer M, Jockusch H, Schmitt-John T., BMC Genet 3(), 2002
PMID: 12174196
Neurofilament homeostasis and motoneurone degeneration.
Perrone Capano C, Pernas-Alonso R, di Porzio U., Bioessays 23(1), 2001
PMID: 11135306
Genetic linkage of Welander distal myopathy to chromosome 2p13.
Ahlberg G, von Tell D, Borg K, Edström L, Anvret M., Ann Neurol 46(3), 1999
PMID: 10482271
Integrated radiation hybrid map of human chromosome 2p13: possible involvement of dynactin in neuromuscular diseases.
Korthaus D, Wedemeyer N, Lengeling A, Ronsiek M, Jockusch H, Schmitt-John T., Genomics 43(2), 1997
PMID: 9244444

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