Characterization of two bioluminescent Rhizobium meliloti strains constructed for field releases

Dammann-Kalinowski T, Niemann S, Keller M, Selbitschka W, Tebbe CC, Pühler A (1996)
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 45(4): 509-512.

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Abstract
The deliberate release of genetically engineered microorganisms requires a thorough characterization of the microbes in question. For the two bioluminescent Rhizobium meliloti strains, L1 and L33 [Selbitschka et al. (1992) Mol Ecol 1:9-19; Selbitschka et al. (1995) FEMS Microbiol Ecol 16:223-232], designated for field release, the sites of genetic modifications in the chromosomes were sequenced from amplified genomic DNA. This indicated no unexpected alterations in the nucleotide sequence. The bioluminescent phenotype was stably inherited over more than 100 generations in liquid cultures. The presence of the luciferase gene in both strains did not have secondary effects on a variety of metabolic pathways as assessed by the Biolog GN system. A specific polymerase chain reaction amplification, based on the chromosomal insertion site of the luc cassette, allowed the discrimination between the two strains and thus simplifies monitoring. The RecA-deficient strain L1 showed a strongly (more than 90%) reduced ability to nodulate alfalfa in competition with its parent strain R. meliloti 2011 and its RecA(+) counterpart L33.
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Dammann-Kalinowski T, Niemann S, Keller M, Selbitschka W, Tebbe CC, Pühler A. Characterization of two bioluminescent Rhizobium meliloti strains constructed for field releases. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 1996;45(4):509-512.
Dammann-Kalinowski, T., Niemann, S., Keller, M., Selbitschka, W., Tebbe, C. C., & Pühler, A. (1996). Characterization of two bioluminescent Rhizobium meliloti strains constructed for field releases. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 45(4), 509-512.
Dammann-Kalinowski, T., Niemann, S., Keller, M., Selbitschka, W., Tebbe, C. C., and Pühler, A. (1996). Characterization of two bioluminescent Rhizobium meliloti strains constructed for field releases. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 45, 509-512.
Dammann-Kalinowski, T., et al., 1996. Characterization of two bioluminescent Rhizobium meliloti strains constructed for field releases. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 45(4), p 509-512.
T. Dammann-Kalinowski, et al., “Characterization of two bioluminescent Rhizobium meliloti strains constructed for field releases”, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 45, 1996, pp. 509-512.
Dammann-Kalinowski, T., Niemann, S., Keller, M., Selbitschka, W., Tebbe, C.C., Pühler, A.: Characterization of two bioluminescent Rhizobium meliloti strains constructed for field releases. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 45, 509-512 (1996).
Dammann-Kalinowski, T, Niemann, S, Keller, M, Selbitschka, Werner, Tebbe, CC, and Pühler, Alfred. “Characterization of two bioluminescent Rhizobium meliloti strains constructed for field releases”. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 45.4 (1996): 509-512.
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7 Citations in Europe PMC

Data provided by Europe PubMed Central.

Soil factors exhibit greater influence than bacterial inoculation on alfalfa growth and nitrogen fixation.
Neumann U, Kosier B, Jahnke J, Priefer UB, Al-Halbouni D., FEMS Microbiol. Ecol. 77(3), 2011
PMID: 21627671
Long-term field release of bioluminescent Sinorhizobium meliloti strains to assess the influence of a recA mutation on the strains' survival.
Selbitschka W, Keller M, Miethling-Graff R, Dresing U, Schwieger F, Krahn I, Homann I, Dammann-Kalinowski T, Puhler A, Tebbe CC., Microb. Ecol. 52(3), 2006
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Diversity of Sinorhizobium meliloti from the Central Asian Alfalfa Gene Center.
Roumiantseva ML, Andronov EE, Sharypova LA, Dammann-Kalinowski T, Keller M, Young JP, Simarov BV., Appl. Environ. Microbiol. 68(9), 2002
PMID: 12200335
Reduced survival of a RecA-deficient Sinorhizobium meliloti strain in sterile and non-sterile during heat stress
Dresing U, Hagen M, Selbitschka W, Puhler A, Keller M., FEMS Microbiol. Ecol. 27(4), 1998
PMID: IND21976940
Evaluation of the recA-based containment system in Rhizobium meliloti GR4
Herrera-Cervera JA, Rodriguez-Alonso FI, Olivares J, Sanjuan J., FEMS Microbiol. Ecol. 22(1), 1997
PMID: IND20576589

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