Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compound N-acetyl-L-phosphinothricin

Kriete G, Niehaus K, Perlick AM, Pühler A, Broer I (1996)
PLANT JOURNAL 9(6): 809-818.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ;
Abstract
A system for the inducible destruction of plant tissues based on the deacetylation of the non-toxic compound N-acetyl-L-phosphinothricin (N-ac-Pt) has been developed. The argE gene product of Escherichia coli, representing a N-acetyl-L-ornithine deacetylase was identified to remove the acetyl-group from N-ac-Pt giving the cytotoxic compound L-phosphinothricin (Pt, glufosinate). Transgenic Nicotiana tabacum plants constitutively expressing the argE gene were constructed. No effect of the bacterial N-acetyl-L-ornithine deacetylase on plant growth and reproduction could be traced. However, application of N-ac-Pt on leaves of the transgenic plants led to the formation of necrotic areas due to the release of Pt. Additionally, due to the uptake of the N-ac-Pt by roots, transgenic shoots grown on medium containing N-ac-Pt bleached within 6-7 days and finally died. Untransformed controls showed no reaction to high amounts of N-ac-Pt applied, either under sterile or under unsterile conditions. In order to construct inducible male-sterile plants, the argE coding region was fused to a DNA fragment carrying sequences homologous to the tobacco TA29 promoter, known to function exclusively in the tapetum. Owing to the tapetum-specific expression of the chimeric gene the application of N-ac-Pt led to empty anthers resulting in male-sterile plants. The sanity of the female reproductive part of the male-sterile flowers could be demonstrated by cross-pollination, Without N-ac-Pt treatment the plants turned out to be completely fertile making fertility restoration in the F-1 generation superflous. The system presented is easy to handle and might be applicable to a wide range of crop plants.
Publishing Year
ISSN
eISSN
PUB-ID

Cite this

Kriete G, Niehaus K, Perlick AM, Pühler A, Broer I. Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compound N-acetyl-L-phosphinothricin. PLANT JOURNAL. 1996;9(6):809-818.
Kriete, G., Niehaus, K., Perlick, A. M., Pühler, A., & Broer, I. (1996). Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compound N-acetyl-L-phosphinothricin. PLANT JOURNAL, 9(6), 809-818.
Kriete, G., Niehaus, K., Perlick, A. M., Pühler, A., and Broer, I. (1996). Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compound N-acetyl-L-phosphinothricin. PLANT JOURNAL 9, 809-818.
Kriete, G., et al., 1996. Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compound N-acetyl-L-phosphinothricin. PLANT JOURNAL, 9(6), p 809-818.
G. Kriete, et al., “Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compound N-acetyl-L-phosphinothricin”, PLANT JOURNAL, vol. 9, 1996, pp. 809-818.
Kriete, G., Niehaus, K., Perlick, A.M., Pühler, A., Broer, I.: Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compound N-acetyl-L-phosphinothricin. PLANT JOURNAL. 9, 809-818 (1996).
Kriete, G, Niehaus, Karsten, Perlick, AM, Pühler, Alfred, and Broer, I. “Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compound N-acetyl-L-phosphinothricin”. PLANT JOURNAL 9.6 (1996): 809-818.
This data publication is cited in the following publications:
This publication cites the following data publications:

24 Citations in Europe PMC

Data provided by Europe PubMed Central.

Temporal and spatial control of gene expression in horticultural crops.
Dutt M, Dhekney SA, Soriano L, Kandel R, Grosser JW., Hortic Res 1(), 2014
PMID: 26504550
Expression of a pathogen-induced cysteine protease (AdCP) in tapetum results in male sterility in transgenic tobacco.
Shukla P, Singh NK, Kumar D, Vijayan S, Ahmed I, Kirti PB., Funct. Integr. Genomics 14(2), 2014
PMID: 24615687
Transcriptomes of the anther sporophyte: availability and uses.
Huang MD, Hsing YI, Huang AH., Plant Cell Physiol. 52(9), 2011
PMID: 21743085
Pollination control technologies for hybrid breeding
Kempe Katja, Gils Mario., Mol. Breed. 27(4), 2011
PMID: IND44515010
Reversible male sterility in eggplant (Solanum melongena L.) by artificial microRNA-mediated silencing of general transcription factor genes.
Toppino L, Kooiker M, Lindner M, Dreni L, Rotino GL, Kater MM., Plant Biotechnol. J. 9(6), 2011
PMID: 20955179
D-glufosinate as a male sterility agent for hybrid seed production.
Hawkes T, Pline-Srnic W, Dale R, Friend E, Hollinshead T, Howe P, Thompson P, Viner R, Greenland A., Plant Biotechnol. J. 9(3), 2011
PMID: 20678098
A novel herbicide-inducible male sterility system
Zhang Jinhui, Zhang Wenlu, Yen Yang, Long Hai, Deng Guangbing, Pan Zhifen, Yu Maoqun., J. Sci. Food Agric. 90(14), 2010
PMID: IND44437558
A novel herbicide-inducible male sterility system.
Zhang J, Zhang W, Yen Y, Long H, Deng G, Pan Z, Yu M., J. Sci. Food Agric. 90(14), 2010
PMID: 20824679
Rapeseed cytoplasm gives advantage in wild relatives and complicates genetically modified crop biocontainment.
Allainguillaume J, Harwood T, Ford CS, Cuccato G, Norris C, Allender CJ, Welters R, King GJ, Wilkinson MJ., New Phytol. 183(4), 2009
PMID: 19496946
Evolution of Glyphosate-Resistant Crop Technology
Green JerryM., Weed Sci. 57(1), 2009
PMID: IND44149067
Expression of sunflower cytoplasmic male sterility-associated open reading frame, orfH522 induces male sterility in transgenic tobacco plants.
Nizampatnam NR, Doodhi H, Kalinati Narasimhan Y, Mulpuri S, Viswanathaswamy DK., Planta 229(4), 2009
PMID: 19151958
RNAi-mediated male sterility of tobacco by silencing TA29.
Nawaz-ul-Rehman MS, Mansoor S, Khan AA, Zafar Y, Briddon RW., Mol. Biotechnol. 36(2), 2007
PMID: 17914195
Suppression and restoration of male fertility using a transcription factor.
Li SF, Iacuone S, Parish RW., Plant Biotechnol. J. 5(2), 2007
PMID: 17309685
Stable male sterility induced by the expression of mutated melon ethylene receptor genes in Nicotiana tabacum
Ishimaru K, Takada K, Watanabe S, Kamada H, Ezura H., Plant Sci. 171(3), 2006
PMID: IND43821112
Selective elimination of perennial ryegrass by activation of a pro-herbicide through engineering E. coli argE gene.
Chen X, Yang W, Sivamani E, Bruneau AH, Wang B, Qu R., Mol. Breed. 15(4), 2005
PMID: IND43789627
A novel, two-component system for cell lethality and its use in engineering nuclear male-sterility in plants.
Burgess DG, Ralston EJ, Hanson WG, Heckert M, Ho M, Jenq T, Palys JM, Tang K, Gutterson N., Plant J. 31(1), 2002
PMID: 12100487
Hybrid seed production and the challenge of propagating male-sterile plants.
Perez-Prat E, van Lookeren Campagne MM., Trends Plant Sci. 7(5), 2002
PMID: 11992824

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 8696362
PubMed | Europe PMC

Search this title in

Google Scholar