Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compound N-acetyl-L-phosphinothricin

Kriete G, Niehaus K, Perlick AM, Pühler A, Broer I (1996)
PLANT JOURNAL 9(6): 809-818.

Download
No fulltext has been uploaded. References only!
Journal Article | Original Article | Published | English

No fulltext has been uploaded

Author
; ; ; ;
Abstract
A system for the inducible destruction of plant tissues based on the deacetylation of the non-toxic compound N-acetyl-L-phosphinothricin (N-ac-Pt) has been developed. The argE gene product of Escherichia coli, representing a N-acetyl-L-ornithine deacetylase was identified to remove the acetyl-group from N-ac-Pt giving the cytotoxic compound L-phosphinothricin (Pt, glufosinate). Transgenic Nicotiana tabacum plants constitutively expressing the argE gene were constructed. No effect of the bacterial N-acetyl-L-ornithine deacetylase on plant growth and reproduction could be traced. However, application of N-ac-Pt on leaves of the transgenic plants led to the formation of necrotic areas due to the release of Pt. Additionally, due to the uptake of the N-ac-Pt by roots, transgenic shoots grown on medium containing N-ac-Pt bleached within 6-7 days and finally died. Untransformed controls showed no reaction to high amounts of N-ac-Pt applied, either under sterile or under unsterile conditions. In order to construct inducible male-sterile plants, the argE coding region was fused to a DNA fragment carrying sequences homologous to the tobacco TA29 promoter, known to function exclusively in the tapetum. Owing to the tapetum-specific expression of the chimeric gene the application of N-ac-Pt led to empty anthers resulting in male-sterile plants. The sanity of the female reproductive part of the male-sterile flowers could be demonstrated by cross-pollination, Without N-ac-Pt treatment the plants turned out to be completely fertile making fertility restoration in the F-1 generation superflous. The system presented is easy to handle and might be applicable to a wide range of crop plants.
Publishing Year
ISSN
eISSN
PUB-ID

Cite this

Kriete G, Niehaus K, Perlick AM, Pühler A, Broer I. Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compound N-acetyl-L-phosphinothricin. PLANT JOURNAL. 1996;9(6):809-818.
Kriete, G., Niehaus, K., Perlick, A. M., Pühler, A., & Broer, I. (1996). Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compound N-acetyl-L-phosphinothricin. PLANT JOURNAL, 9(6), 809-818. doi:10.1046/j.1365-313X.1996.9060809.x
Kriete, G., Niehaus, K., Perlick, A. M., Pühler, A., and Broer, I. (1996). Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compound N-acetyl-L-phosphinothricin. PLANT JOURNAL 9, 809-818.
Kriete, G., et al., 1996. Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compound N-acetyl-L-phosphinothricin. PLANT JOURNAL, 9(6), p 809-818.
G. Kriete, et al., “Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compound N-acetyl-L-phosphinothricin”, PLANT JOURNAL, vol. 9, 1996, pp. 809-818.
Kriete, G., Niehaus, K., Perlick, A.M., Pühler, A., Broer, I.: Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compound N-acetyl-L-phosphinothricin. PLANT JOURNAL. 9, 809-818 (1996).
Kriete, G, Niehaus, Karsten, Perlick, AM, Pühler, Alfred, and Broer, I. “Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compound N-acetyl-L-phosphinothricin”. PLANT JOURNAL 9.6 (1996): 809-818.
This data publication is cited in the following publications:
This publication cites the following data publications:

29 Citations in Europe PMC

Data provided by Europe PubMed Central.

Hybrid seed production and the challenge of propagating male-sterile plants.
Perez-Prat E, van Lookeren Campagne MM., Trends Plant Sci 7(5), 2002
PMID: 11992824
A novel, two-component system for cell lethality and its use in engineering nuclear male-sterility in plants.
Burgess DG, Ralston EJ, Hanson WG, Heckert M, Ho M, Jenq T, Palys JM, Tang K, Gutterson N., Plant J 31(1), 2002
PMID: 12100487

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 8696362
PubMed | Europe PMC

Search this title in

Google Scholar