A physical and genetic map of the Corynebacterium glutamicum ATCC 13032 chromosome

Bathe B, Kalinowski J, Pühler A (1996)
MOLECULAR & GENERAL GENETICS 252(3): 255-265.

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Abstract
A combined physical and genetic map of the Corynebacterium glutamicum ATCC 13032 chromosome was constructed using pulsed-field gel electrophoresis (PFGE) and hybridizations with cloned gene probes. Total genomic DNA was digested with the meganucleases (5'-ATTTAAAT-3'), PacI SwaI (5'-TTAATTAA-3') and PmeI (5'-GTTTAAAC-3') yielding 26,27, and 23 fragments, respectively. The chromosomal restriction fragments were then separated by PFGE. By summing up the lengths of the fragments generated with each of the three enzymes, a genome size of 3082 +/- 20 kb was determined. To identify adjacent SwaI fragments, a genomic cosmid library of C. glutamicum was screened for chromosomal inserts containing SwaI sites. Southern blots of the PFGE gels were hybridized with these linking clones to connect the SwaI fragments in their natural order. By this method, about 90% of the genome could be ordered into three contigs. Two of the remaining gaps were closed by cross-hybridization of blotted SwaI digests using as probes PacI and PmeI fragments isolated from PFGE gels. The last gap in the chromosomal map was closed by hybridization experiments using partial SwaI digestions, thereby proving the circularity of the chromosome. By hybridization of gene probes to SwaI fragments separated by PFGE about 30 genes; including rRNA operons, IS element and transposon insertions were localized on the physical map.
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Bathe B, Kalinowski J, Pühler A. A physical and genetic map of the Corynebacterium glutamicum ATCC 13032 chromosome. MOLECULAR & GENERAL GENETICS. 1996;252(3):255-265.
Bathe, B., Kalinowski, J., & Pühler, A. (1996). A physical and genetic map of the Corynebacterium glutamicum ATCC 13032 chromosome. MOLECULAR & GENERAL GENETICS, 252(3), 255-265.
Bathe, B., Kalinowski, J., and Pühler, A. (1996). A physical and genetic map of the Corynebacterium glutamicum ATCC 13032 chromosome. MOLECULAR & GENERAL GENETICS 252, 255-265.
Bathe, B., Kalinowski, J., & Pühler, A., 1996. A physical and genetic map of the Corynebacterium glutamicum ATCC 13032 chromosome. MOLECULAR & GENERAL GENETICS, 252(3), p 255-265.
B. Bathe, J. Kalinowski, and A. Pühler, “A physical and genetic map of the Corynebacterium glutamicum ATCC 13032 chromosome”, MOLECULAR & GENERAL GENETICS, vol. 252, 1996, pp. 255-265.
Bathe, B., Kalinowski, J., Pühler, A.: A physical and genetic map of the Corynebacterium glutamicum ATCC 13032 chromosome. MOLECULAR & GENERAL GENETICS. 252, 255-265 (1996).
Bathe, B., Kalinowski, Jörn, and Pühler, Alfred. “A physical and genetic map of the Corynebacterium glutamicum ATCC 13032 chromosome”. MOLECULAR & GENERAL GENETICS 252.3 (1996): 255-265.
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57 References

Data provided by Europe PubMed Central.

The chromosomal DNA of Streptomyces lividans 66 is linear.
Lin YS, Kieser HM, Hopwood DA, Chen CW., Mol. Microbiol. 10(5), 1993
PMID: 7934869
Isolation and characterization of IS31831, a transposable element from Corynebacterium glutamicum.
Vertes AA, Inui M, Kobayashi M, Kurusu Y, Yukawa H., Mol. Microbiol. 11(4), 1994
PMID: 8196545
Restriction endonucleases for pulsed field mapping of bacterial genomes.
McClelland M, Jones R, Patel Y, Nelson M., Nucleic Acids Res. 15(15), 1987
PMID: 2819819
Nucleotide sequence of the meso-diaminopimelate D-dehydrogenase gene from Corynebacterium glutamicum.
Ishino S, Mizukami T, Yamaguchi K, Katsumata R, Araki K., Nucleic Acids Res. 15(9), 1987
PMID: 3588313
McrA and McrB restriction phenotypes of some E. coli strains and implications for gene cloning.
Raleigh EA, Murray NE, Revel H, Blumenthal RM, Westaway D, Reith AD, Rigby PW, Elhai J, Hanahan D., Nucleic Acids Res. 16(4), 1988
PMID: 2831502

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