Comparative characterization of H2 production by the conventional Mo nitrogenase and the alternative ''iron only'' nitrogenase of Rhodobacter capsulatus hup(-) mutants

Krahn E, Schneider K, Müller A (1996)
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 46(3): 285-290.

Journal Article | Published | English

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Abstract
Cell suspensions of uptake-hydrogenase-deficient (hup(-)) mutants of a wild-type (B10S) and a nifHDK deletion strain of Rhodobacter capsulatus were used comparatively to characterize the conventional, Mo-containing and the alternative, ''iron-only'' nitrogenase of this organism by determining the H-2 production and acetylene reduction activities under argon and dinitrogen atmospheres. A comparison with the corresponding hup(+) strains revealed that the hup(-) mutation did not affect the nitrogenase activity and specificity within the acetylene-reduction assay, but caused a significant increase in H-2 production, which was more prominent in the case of the Delta nifHDK strain. The Delta nifHDK hup(-) cells, grown in Mo-depleted medium and, thus, expressing the alternative nitrogenase system, were more than ten times less active in the acetylene-reduction assay but exhibited H-2 production rates equivalent to about 60% of the rates obtained with B10S hup(-) cells after growth in a medium containing 10 mu M MoO4-. When these conditions were applied, the BIOS strain only expressed the Mo nitrogenase. Under an argon atmosphere containing about 5.5% (v/v) acetylene and under a dinitrogen atmosphere, Delta nifHDK hup(-) cells produced H-2 at even higher rates than B10S hup(-) cells. The implications of our findings on a possible biotechnological H-2 production and on the mechanism of nitrogenase catalysis are considered.
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Krahn E, Schneider K, Müller A. Comparative characterization of H2 production by the conventional Mo nitrogenase and the alternative ''iron only'' nitrogenase of Rhodobacter capsulatus hup(-) mutants. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 1996;46(3):285-290.
Krahn, E., Schneider, K., & Müller, A. (1996). Comparative characterization of H2 production by the conventional Mo nitrogenase and the alternative ''iron only'' nitrogenase of Rhodobacter capsulatus hup(-) mutants. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 46(3), 285-290.
Krahn, E., Schneider, K., and Müller, A. (1996). Comparative characterization of H2 production by the conventional Mo nitrogenase and the alternative ''iron only'' nitrogenase of Rhodobacter capsulatus hup(-) mutants. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 46, 285-290.
Krahn, E., Schneider, K., & Müller, A., 1996. Comparative characterization of H2 production by the conventional Mo nitrogenase and the alternative ''iron only'' nitrogenase of Rhodobacter capsulatus hup(-) mutants. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 46(3), p 285-290.
E. Krahn, K. Schneider, and A. Müller, “Comparative characterization of H2 production by the conventional Mo nitrogenase and the alternative ''iron only'' nitrogenase of Rhodobacter capsulatus hup(-) mutants”, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 46, 1996, pp. 285-290.
Krahn, E., Schneider, K., Müller, A.: Comparative characterization of H2 production by the conventional Mo nitrogenase and the alternative ''iron only'' nitrogenase of Rhodobacter capsulatus hup(-) mutants. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 46, 285-290 (1996).
Krahn, E, Schneider, Klaus, and Müller, Achim. “Comparative characterization of H2 production by the conventional Mo nitrogenase and the alternative ''iron only'' nitrogenase of Rhodobacter capsulatus hup(-) mutants”. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 46.3 (1996): 285-290.
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