The modular nodulins Nvf-28/32 of broad bean (Vicia faba L): Alternative exon combinations account for different modular structures

Küster H, Fruhling M, Pühler A, Perlick AM (1996)
MOLECULAR & GENERAL GENETICS 252(6): 648-657.

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Abstract
The broad bean late nodulins, Nvf-28/32, are composed of two types of repetitively occurring sequence modules flanked by unique N- and C-terminal modules. Six isoforms of these nodulins were characterized by a specific modular structure resulting from a different individual order of repetitive sequence modules. A detailed analysis of genomic PCR fragments revealed that the repetitive modules and the N-terminal unique module exactly corresponded to exons, whereas the C-terminal module was specified by two exons. Since those exons encoding the repetitive modules missing in specific Nvf-28/32 isoforms were consistently present within genomic sequences, a posttranscriptional generation of VfNOD28/32 transcripts specifying six Nvf-28/32 nodulins was concluded. Using tissue-print hybridizations, these transcripts were localized in the interzone II-III and the nitrogen-fixing zone III of root nodules. From this and from cDNA-cDNA hybridizations demonstrating a comparable timing of expression of VfNOD28/32 and of leghemoglobin transcripts in root nodules, a function of the modular nodulins Nvf-28/32 in late developmental stages of broad bean nodules was inferred.
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Küster H, Fruhling M, Pühler A, Perlick AM. The modular nodulins Nvf-28/32 of broad bean (Vicia faba L): Alternative exon combinations account for different modular structures. MOLECULAR & GENERAL GENETICS. 1996;252(6):648-657.
Küster, H., Fruhling, M., Pühler, A., & Perlick, A. M. (1996). The modular nodulins Nvf-28/32 of broad bean (Vicia faba L): Alternative exon combinations account for different modular structures. MOLECULAR & GENERAL GENETICS, 252(6), 648-657.
Küster, H., Fruhling, M., Pühler, A., and Perlick, A. M. (1996). The modular nodulins Nvf-28/32 of broad bean (Vicia faba L): Alternative exon combinations account for different modular structures. MOLECULAR & GENERAL GENETICS 252, 648-657.
Küster, H., et al., 1996. The modular nodulins Nvf-28/32 of broad bean (Vicia faba L): Alternative exon combinations account for different modular structures. MOLECULAR & GENERAL GENETICS, 252(6), p 648-657.
H. Küster, et al., “The modular nodulins Nvf-28/32 of broad bean (Vicia faba L): Alternative exon combinations account for different modular structures”, MOLECULAR & GENERAL GENETICS, vol. 252, 1996, pp. 648-657.
Küster, H., Fruhling, M., Pühler, A., Perlick, A.M.: The modular nodulins Nvf-28/32 of broad bean (Vicia faba L): Alternative exon combinations account for different modular structures. MOLECULAR & GENERAL GENETICS. 252, 648-657 (1996).
Küster, Helge, Fruhling, M, Pühler, Alfred, and Perlick, AM. “The modular nodulins Nvf-28/32 of broad bean (Vicia faba L): Alternative exon combinations account for different modular structures”. MOLECULAR & GENERAL GENETICS 252.6 (1996): 648-657.
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