Influence of heavy metal ions on antibodies and immune complexes investigated by dynamic light scattering and enzyme-linked immunosorbent assay

Bauer R, Müller A, Richter M, Schneider K, Frey J, Engelhardt W (1997)
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1334(1): 98-108.

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Abstract
The effect of Cd2+, Pb2+, Hg2+ and Cu2+ on the aggregation behaviour of monoclonal rat-IgG1-anti-mouse antibodies (kappa-light chain specific) and their antibody-antigen complexes with monoclonal mouse-IgG1 is reported. Investigations were done using the dynamic light scattering method. Cd2+ ions affected the hydrodynamic properties of the antibodies and the immune complex formation very little. More than 4 Cu2+ ions per antibody molecule led to large insoluble aggregates. Pb2+ ions also interacted with antibodies and immune complexes. Instead of 'monomeric' antibodies (Ab) or immune complexes (Ab(1)Ag(1)), large soluble aggregates were detectable in the solution. Hg2+ ions induced complex formation with 3-4 antibodies per aggregate. Possible kinds of interaction are discussed. Additionally, we tested the antigen binding activity of metal-treated antibodies in ELISA-tests. The Sandwich ELISA technique was used to investigate the serological activity of the metal-treated antibodies, i.e., the reaction with the specific antigen. For these experiments we used the same monoclonal antibodies, mouse-IgG1 and rat-IgG1-anti-mouse. The influence of the above mentioned heavy metal ions was investigated up to a 10-fold molar excess over the antibody concentration. Even at these 'unphysiological' high metal ion concentrations an inhibition of the antibody-antigen binding activity was not detectable.
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Bauer R, Müller A, Richter M, Schneider K, Frey J, Engelhardt W. Influence of heavy metal ions on antibodies and immune complexes investigated by dynamic light scattering and enzyme-linked immunosorbent assay. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS. 1997;1334(1):98-108.
Bauer, R., Müller, A., Richter, M., Schneider, K., Frey, J., & Engelhardt, W. (1997). Influence of heavy metal ions on antibodies and immune complexes investigated by dynamic light scattering and enzyme-linked immunosorbent assay. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1334(1), 98-108.
Bauer, R., Müller, A., Richter, M., Schneider, K., Frey, J., and Engelhardt, W. (1997). Influence of heavy metal ions on antibodies and immune complexes investigated by dynamic light scattering and enzyme-linked immunosorbent assay. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1334, 98-108.
Bauer, R., et al., 1997. Influence of heavy metal ions on antibodies and immune complexes investigated by dynamic light scattering and enzyme-linked immunosorbent assay. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1334(1), p 98-108.
R. Bauer, et al., “Influence of heavy metal ions on antibodies and immune complexes investigated by dynamic light scattering and enzyme-linked immunosorbent assay”, BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, vol. 1334, 1997, pp. 98-108.
Bauer, R., Müller, A., Richter, M., Schneider, K., Frey, J., Engelhardt, W.: Influence of heavy metal ions on antibodies and immune complexes investigated by dynamic light scattering and enzyme-linked immunosorbent assay. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS. 1334, 98-108 (1997).
Bauer, R, Müller, Achim, Richter, M, Schneider, Klaus, Frey, Jürgen, and Engelhardt, W. “Influence of heavy metal ions on antibodies and immune complexes investigated by dynamic light scattering and enzyme-linked immunosorbent assay”. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1334.1 (1997): 98-108.
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