Extracellular production of a hybrid beta-glucanase from Bacillus by Escherichia coli under different cultivation conditions in shaking cultures and bioreactors

Miksch G, Neitzel R, Fiedler E, Friehs K, Flaschel E (1997)
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 47(2): 120-126.

Journal Article | Published | English

No fulltext has been uploaded

Author
Abstract
Cultivation conditions for the extracellular production of a hybrid beta-glucanase from Bacillus were established by using Escherichia coli JM109 carrying the plasmid pLF3. This plasmid contained a novel secretion system consisting of the kil gene (killing protein) of plasmid ColE1 under the stationary-phase promoter of either the Jic or the bolA gene, an omega interposon (Prentki and Krisch 1984) located upstream of the promoters and a hybrid beta-glucanase gene of Bacillus. When controlled by the Jic promoter, the kil gene led to a higher total production of beta-glucanase and a higher protein secretion than when it was under control of the bolA promoter. When the effect of different distances between the stationary-phase promoters and the kil gene was investigated, a shorter distance was generally found to result in a higher secretion. With a complex growth medium, the kinetics of extracellular production of the enzyme depended on several operating variables, such as the salt concentration (NaCl) and the oxygen supply, which were varied by changing the culture volume and the shaking speed. In defined media the secretion of beta-glucanase into the medium was increased significantly by the addition of glycerol as a carbon source and by prolonged cultivation. The strain with the highest production and secretion yield of beta-glucanase [E. coli JM109(pLF3)] was tested on the fermenter scale.
Publishing Year
ISSN
eISSN
PUB-ID

Cite this

Miksch G, Neitzel R, Fiedler E, Friehs K, Flaschel E. Extracellular production of a hybrid beta-glucanase from Bacillus by Escherichia coli under different cultivation conditions in shaking cultures and bioreactors. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 1997;47(2):120-126.
Miksch, G., Neitzel, R., Fiedler, E., Friehs, K., & Flaschel, E. (1997). Extracellular production of a hybrid beta-glucanase from Bacillus by Escherichia coli under different cultivation conditions in shaking cultures and bioreactors. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 47(2), 120-126.
Miksch, G., Neitzel, R., Fiedler, E., Friehs, K., and Flaschel, E. (1997). Extracellular production of a hybrid beta-glucanase from Bacillus by Escherichia coli under different cultivation conditions in shaking cultures and bioreactors. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 47, 120-126.
Miksch, G., et al., 1997. Extracellular production of a hybrid beta-glucanase from Bacillus by Escherichia coli under different cultivation conditions in shaking cultures and bioreactors. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 47(2), p 120-126.
G. Miksch, et al., “Extracellular production of a hybrid beta-glucanase from Bacillus by Escherichia coli under different cultivation conditions in shaking cultures and bioreactors”, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 47, 1997, pp. 120-126.
Miksch, G., Neitzel, R., Fiedler, E., Friehs, K., Flaschel, E.: Extracellular production of a hybrid beta-glucanase from Bacillus by Escherichia coli under different cultivation conditions in shaking cultures and bioreactors. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 47, 120-126 (1997).
Miksch, Gerd, Neitzel, R, Fiedler, E, Friehs, Karl, and Flaschel, Erwin. “Extracellular production of a hybrid beta-glucanase from Bacillus by Escherichia coli under different cultivation conditions in shaking cultures and bioreactors”. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 47.2 (1997): 120-126.
This data publication is cited in the following publications:
This publication cites the following data publications:

14 Citations in Europe PMC

Data provided by Europe PubMed Central.

Delineation of the translocation of colicin E7 across the inner membrane of Escherichia coli.
Chen YR, Yang TY, Lei GS, Lin LJ, Chak KF., Arch. Microbiol. 193(6), 2011
PMID: 21387181
Optimization of glutaryl-7-aminocephalosporanic acid acylase expression in E. coli.
Volonte F, Marinelli F, Gastaldo L, Sacchi S, Pilone MS, Pollegioni L, Molla G., Protein Expr. Purif. 61(2), 2008
PMID: 18586517
In vitro and in vivo characteristics of bacterial phytases and their efficacy in broiler chickens.
Elkhalil EA, Manner K, Borriss R, Simon O., Br. Poult. Sci. 48(1), 2007
PMID: 17364542
Recombinant protein secretion in Escherichia coli.
Mergulhao FJ, Summers DK, Monteiro GA., Biotechnol. Adv. 23(3), 2005
PMID: 15763404
Extracellular expression and single step purification of recombinant Escherichia coli L-asparaginase II.
Khushoo A, Pal Y, Singh BN, Mukherjee KJ., Protein Expr. Purif. 38(1), 2004
PMID: 15477079
Improvement of posttranslational bottlenecks in the production of penicillin amidase in recombinant Escherichia coli strains.
Ignatova Z, Mahsunah A, Georgieva M, Kasche V., Appl. Environ. Microbiol. 69(2), 2003
PMID: 12571052
Optimization of the extracellular production of a bacterial phytase with Escherichia coli by using different fed-batch fermentation strategies.
Kleist S, Miksch G, Hitzmann B, Arndt M, Friehs K, Flaschel E., Appl. Microbiol. Biotechnol. 61(5-6), 2003
PMID: 12764560
Advances in Escherichia coli production of therapeutic proteins.
Swartz JR., Curr. Opin. Biotechnol. 12(2), 2001
PMID: 11287237

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 9077001
PubMed | Europe PMC

Search this title in

Google Scholar