The regulatory protein MucR binds to a short DNA region located upstream of the mucR coding region in Rhizobium meliloti

Bertram-Drogatz PA, Rüberg S, Becker A, Pühler A (1997)
Mol Gen Genet 254(5): 529-538.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
The Rhizobium meliloti MucR protein is known to regulate the biosynthesis of the two exopolysaccharides, succinoglycan and galactoglucan. The mucR gene was successfully overexpressed in Escherichia coli BL21 cells by heat shock induction using a two-plasmid system. Cell extracts of the production strain contained about 20% of a polypeptide of 17 kDa apparent molecular mass, corresponding to the size expected for MucR. As shown by an electrophoretic mobility shift assay, these extracts were active in the specific retardation of a 219-bp DNA fragment including 134-bp of the non-coding region upstream of the mucR gene. Primer extension analysis showed that this DNA fragment was located within the transcribed region upstream of the mucR gene. Competition experiments revealed that a 44-bp sequence present within the 134-bp upstream of the mucR gene contained the MucR binding site. Although binding of MucR to this site exhibited a moderate dissociation constant of K-d approximate to 1.4 x 10(-7) M, the reaction was highly specific since fragments containing binding sites for the homologous Ros protein from Agrobacterium tumefaciens were not able to compete for MucR binding.
Erscheinungsjahr
Zeitschriftentitel
Mol Gen Genet
Band
254
Zeitschriftennummer
5
Seite
529-538
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eISSN
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Bertram-Drogatz PA, Rüberg S, Becker A, Pühler A. The regulatory protein MucR binds to a short DNA region located upstream of the mucR coding region in Rhizobium meliloti. Mol Gen Genet. 1997;254(5):529-538.
Bertram-Drogatz, P. A., Rüberg, S., Becker, A., & Pühler, A. (1997). The regulatory protein MucR binds to a short DNA region located upstream of the mucR coding region in Rhizobium meliloti. Mol Gen Genet, 254(5), 529-538. doi:10.1007/s004380050448
Bertram-Drogatz, P. A., Rüberg, S., Becker, A., and Pühler, A. (1997). The regulatory protein MucR binds to a short DNA region located upstream of the mucR coding region in Rhizobium meliloti. Mol Gen Genet 254, 529-538.
Bertram-Drogatz, P.A., et al., 1997. The regulatory protein MucR binds to a short DNA region located upstream of the mucR coding region in Rhizobium meliloti. Mol Gen Genet, 254(5), p 529-538.
P.A. Bertram-Drogatz, et al., “The regulatory protein MucR binds to a short DNA region located upstream of the mucR coding region in Rhizobium meliloti”, Mol Gen Genet, vol. 254, 1997, pp. 529-538.
Bertram-Drogatz, P.A., Rüberg, S., Becker, A., Pühler, A.: The regulatory protein MucR binds to a short DNA region located upstream of the mucR coding region in Rhizobium meliloti. Mol Gen Genet. 254, 529-538 (1997).
Bertram-Drogatz, P. A., Rüberg, Silvia, Becker, Anke, and Pühler, Alfred. “The regulatory protein MucR binds to a short DNA region located upstream of the mucR coding region in Rhizobium meliloti”. Mol Gen Genet 254.5 (1997): 529-538.

18 Zitationen in Europe PMC

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Rhizobial exopolysaccharides: genetic control and symbiotic functions.
Skorupska A, Janczarek M, Marczak M, Mazur A, Król J., Microb Cell Fact 5(), 2006
PMID: 16483356
MucR is necessary for galactoglucan production in Sinorhizobium meliloti EFB1.
Martín M, Lloret J, Sánchez-Contreras M, Bonilla I, Rivilla R., Mol Plant Microbe Interact 13(1), 2000
PMID: 10656595
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Mendrygal KE, González JE., J Bacteriol 182(3), 2000
PMID: 10633091
The origin of prokaryotic C2H2 zinc finger regulators.
Bouhouche N, Syvanen M, Kado CI., Trends Microbiol 8(2), 2000
PMID: 10664601
Identification of genes in the RosR regulon of Rhizobium etli.
Bittinger MA, Handelsman J., J Bacteriol 182(6), 2000
PMID: 10692377
BioS, a biotin-induced, stationary-phase, and possible LysR-type regulator in Sinorhizobium meliloti.
Heinz EB, Phillips DA, Streit WR., Mol Plant Microbe Interact 12(9), 1999
PMID: 10494632
Iron-dependent transcription of the regulatory gene ros of Agrobacterium radiobacter.
Hussain H, Johnston AW., Mol Plant Microbe Interact 10(9), 1997
PMID: 9390423

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