A Novel Family of Plant DNA-Binding Proteins Containing both HMG-Box and AT-Rich Interaction Domains

Hansen FT, Madsen CK, Nordland AM, Grasser M, Merkle T, Grasser KD (2008)
Biochemistry 47(50): 13207-13214.

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Abstract
The A/T-rich interaction domain (ARID) and the HMG-box domain represent DNA-interaction modules that are found in sequence-specific as well as nonsequence-specific DNA-binding proteins. Both domains are found in a variety of DNA-interacting proteins in a wide range of eukaryotic organisms. Proteins that contain both an ARID and an HMG-box domain, here termed ARID-HMG proteins, appear to be specific for plants. This protein family is conserved in higher plants (both mono- and dicot plants) as well as lower plants such as the moss Physcomitrella. Since ARID-HMG proteins have not been studied experimentally, we have examined here two family members from Arabidopsis. The genes encoding ARID-HMG1 and ARID-HMG2 are widely expressed in Arabidopsis but at different levels. Subcellular localization experiments studying ARID-HMG1 and ARID-HMG2 fused to GFP by fluorescence microscopy show that both proteins localize primarily to cell nuclei. Analyses of the DNA-binding properties using electrophoretic mobility shift assays revealed that mediated by the HMG-box domain, ARID-HMG I binds structure specifically to DNA minicircles. Mediated by the ARID, the protein binds preferentially to A/T-rich DNA, when compared with G/C-rich DNA. Therefore, both DNA-binding domains contribute to the DNA interactions of ARID-HMGI. Accordingly, the protein combines DNA-binding properties characteristic of ARID and HMG-box proteins.
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Hansen FT, Madsen CK, Nordland AM, Grasser M, Merkle T, Grasser KD. A Novel Family of Plant DNA-Binding Proteins Containing both HMG-Box and AT-Rich Interaction Domains. Biochemistry. 2008;47(50):13207-13214.
Hansen, F. T., Madsen, C. K., Nordland, A. M., Grasser, M., Merkle, T., & Grasser, K. D. (2008). A Novel Family of Plant DNA-Binding Proteins Containing both HMG-Box and AT-Rich Interaction Domains. Biochemistry, 47(50), 13207-13214.
Hansen, F. T., Madsen, C. K., Nordland, A. M., Grasser, M., Merkle, T., and Grasser, K. D. (2008). A Novel Family of Plant DNA-Binding Proteins Containing both HMG-Box and AT-Rich Interaction Domains. Biochemistry 47, 13207-13214.
Hansen, F.T., et al., 2008. A Novel Family of Plant DNA-Binding Proteins Containing both HMG-Box and AT-Rich Interaction Domains. Biochemistry, 47(50), p 13207-13214.
F.T. Hansen, et al., “A Novel Family of Plant DNA-Binding Proteins Containing both HMG-Box and AT-Rich Interaction Domains”, Biochemistry, vol. 47, 2008, pp. 13207-13214.
Hansen, F.T., Madsen, C.K., Nordland, A.M., Grasser, M., Merkle, T., Grasser, K.D.: A Novel Family of Plant DNA-Binding Proteins Containing both HMG-Box and AT-Rich Interaction Domains. Biochemistry. 47, 13207-13214 (2008).
Hansen, Frederik T., Madsen, Claus K., Nordland, Anne Mette, Grasser, Marion, Merkle, Thomas, and Grasser, Klaus D. “A Novel Family of Plant DNA-Binding Proteins Containing both HMG-Box and AT-Rich Interaction Domains”. Biochemistry 47.50 (2008): 13207-13214.
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