Fluorescent proteins for single-molecule fluorescence applications

Seefeldt B, Kasper R, Seidel T, Tinnefeld P, Dietz K-J, Heilemann M, Sauer M (2008)
JOURNAL OF BIOPHOTONICS 1(1): 74-82.

Journal Article | Published | English

No fulltext has been uploaded

Abstract
We present single-molecule fluorescence data of fluorescent proteins GFP, YFP, DsRed, and mCherry, a new derivative of DsRed. Ensemble and single-molecule fluorescence experiments proved mCherry as an ideally suited fluorophore for single-molecule applications, demonstrated by high photostability and rare fluorescence-intensity fluctuations. Although mCherry exhibits the lowest fluorescence quantum yield among the fluorescent proteins investigated, its superior photophysical characteristics suggest mCherry as an ideal alternative in single-molecule fluorescence experiments. Due to its spectral characteristics and short fluorescence lifetime of 1.46 ns, mCherry complements other existing fluorescent proteins and is recommended for tracking and localization of target molecules with high accuracy, fluorescence resonance energy transfer (FRET), fluorescence lifetime imaging microscopy (FLIM), or multicolor applications. [GRAPHICS] mCherry exhibits a relatively high photostability and brightness under single-molecule fluorescence imaging conditions. (C) 2008 by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Publishing Year
ISSN
eISSN
PUB-ID

Cite this

Seefeldt B, Kasper R, Seidel T, et al. Fluorescent proteins for single-molecule fluorescence applications. JOURNAL OF BIOPHOTONICS. 2008;1(1):74-82.
Seefeldt, B., Kasper, R., Seidel, T., Tinnefeld, P., Dietz, K. - J., Heilemann, M., & Sauer, M. (2008). Fluorescent proteins for single-molecule fluorescence applications. JOURNAL OF BIOPHOTONICS, 1(1), 74-82.
Seefeldt, B., Kasper, R., Seidel, T., Tinnefeld, P., Dietz, K. - J., Heilemann, M., and Sauer, M. (2008). Fluorescent proteins for single-molecule fluorescence applications. JOURNAL OF BIOPHOTONICS 1, 74-82.
Seefeldt, B., et al., 2008. Fluorescent proteins for single-molecule fluorescence applications. JOURNAL OF BIOPHOTONICS, 1(1), p 74-82.
B. Seefeldt, et al., “Fluorescent proteins for single-molecule fluorescence applications”, JOURNAL OF BIOPHOTONICS, vol. 1, 2008, pp. 74-82.
Seefeldt, B., Kasper, R., Seidel, T., Tinnefeld, P., Dietz, K.-J., Heilemann, M., Sauer, M.: Fluorescent proteins for single-molecule fluorescence applications. JOURNAL OF BIOPHOTONICS. 1, 74-82 (2008).
Seefeldt, Britta, Kasper, Robert, Seidel, Thorsten, Tinnefeld, Philip, Dietz, Karl-Josef, Heilemann, Mike, and Sauer, Markus. “Fluorescent proteins for single-molecule fluorescence applications”. JOURNAL OF BIOPHOTONICS 1.1 (2008): 74-82.
This data publication is cited in the following publications:
This publication cites the following data publications:

16 Citations in Europe PMC

Data provided by Europe PubMed Central.

Ligand-induced dynamics of neurotrophin receptors investigated by single-molecule imaging approaches.
Marchetti L, Luin S, Bonsignore F, de Nadai T, Beltram F, Cattaneo A., Int J Mol Sci 16(1), 2015
PMID: 25603178
Photophysical processes in single molecule organic fluorescent probes.
Stennett EM, Ciuba MA, Levitus M., Chem Soc Rev 43(4), 2014
PMID: 24141280
Quantification of Forster resonance energy transfer by monitoring sensitized emission in living plant cells.
Muller SM, Galliardt H, Schneider J, Barisas BG, Seidel T., Front Plant Sci 4(), 2013
PMID: 24194740
Subcellular localization-dependent changes in EGFP fluorescence lifetime measured by time-resolved flow cytometry.
Gohar AV, Cao R, Jenkins P, Li W, Houston JP, Houston KD., Biomed Opt Express 4(8), 2013
PMID: 24010001
H2A.Z acidic patch couples chromatin dynamics to regulation of gene expression programs during ESC differentiation.
Subramanian V, Mazumder A, Surface LE, Butty VL, Fields PA, Alwan A, Torrey L, Thai KK, Levine SS, Bathe M, Boyer LA., PLoS Genet. 9(8), 2013
PMID: 23990805
Appraisal of a Leishmania major strain stably expressing mCherry fluorescent protein for both in vitro and in vivo studies of potential drugs and vaccine against cutaneous leishmaniasis.
Calvo-Alvarez E, Guerrero NA, Alvarez-Velilla R, Prada CF, Requena JM, Punzon C, Llamas MA, Arevalo FJ, Rivas L, Fresno M, Perez-Pertejo Y, Balana-Fouce R, Reguera RM., PLoS Negl Trop Dis 6(11), 2012
PMID: 23209866
Neurotrophin signaling via long-distance axonal transport.
Chowdary PD, Che DL, Cui B., Annu Rev Phys Chem 63(), 2012
PMID: 22404590
The cellular energization state affects peripheral stalk stability of plant vacuolar H+-ATPase and impairs vacuolar acidification.
Schnitzer D, Seidel T, Sander T, Golldack D, Dietz KJ., Plant Cell Physiol. 52(5), 2011
PMID: 21474463
Multimodality imaging of gene transfer with a receptor-based reporter gene.
Chen R, Parry JJ, Akers WJ, Berezin MY, El Naqa IM, Achilefu S, Edwards WB, Rogers BE., J. Nucl. Med. 51(9), 2010
PMID: 20720053
Fluorescent proteins as light-inducible photochemical partners.
Lukyanov KA, Serebrovskaya EO, Lukyanov S, Chudakov DM., Photochem. Photobiol. Sci. 9(10), 2010
PMID: 20672171
Fluorescence lifetime measurements and biological imaging.
Berezin MY, Achilefu S., Chem. Rev. 110(5), 2010
PMID: 20356094
Multiple redox and non-redox interactions define 2-Cys peroxiredoxin as a regulatory hub in the chloroplast.
Muthuramalingam M, Seidel T, Laxa M, Nunes de Miranda SM, Gartner F, Stroher E, Kandlbinder A, Dietz KJ., Mol Plant 2(6), 2009
PMID: 19995730
Redox-dependent regulation of the stress-induced zinc-finger protein SAP12 in Arabidopsis thaliana.
Stroher E, Wang XJ, Roloff N, Klein P, Husemann A, Dietz KJ., Mol Plant 2(2), 2009
PMID: 19825620
Single-molecule spectroscopy of fluorescent proteins.
Blum C, Subramaniam V., Anal Bioanal Chem 393(2), 2009
PMID: 18854990

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 19343637
PubMed | Europe PMC

Search this title in

Google Scholar