Metabolomic and genetic analyses of flavonol synthesis in Arabidopsis thaliana support the in vivo involvement of leucoanthocyanidin dioxygenase

Stracke R, De Vos RCH, Bartelniewoehner L, Ishihara H, Sagasser M, Martens S, Weisshaar B (2009)
Planta 229(2): 427-445.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
; ; ; ; ; ;
Abstract / Bemerkung
Flavonol synthase (FLS) (EC-number 1.14.11.23), the enzyme that catalyses the conversion of flavonols into dihydroflavonols, is part of the flavonoid biosynthesis pathway. In Arabidopsis thaliana, this activity is thought to be encoded by several loci. In addition to the FLAVONOL SYNTHASE1 (FLS1) locus that has been confirmed by enzyme activity assays, loci displaying similarity of the deduced amino acid sequences to FLS1 have been identified. We studied the putative A. thaliana FLS gene family using a combination of genetic and metabolite analysis approaches. Although several of the FLS gene family members are expressed, only FLS1 appeared to influence flavonoid biosynthesis. Seedlings of an A. thaliana fls1 null mutant (fls1-2) show enhanced anthocyanin levels, drastic reduction in flavonol glycoside content and concomitant accumulation of glycosylated forms of dihydroflavonols, the substrate of the FLS reaction. By using a leucoanthocyanidin dioxygenase (ldox) fls1-2 double mutant, we present evidence that the remaining flavonol glycosides found in the fls1-2 mutant are synthesized in planta by the FLS-like side activity of the LDOX enzyme.
Erscheinungsjahr
Zeitschriftentitel
Planta
Band
229
Zeitschriftennummer
2
Seite
427-445
ISSN
eISSN
PUB-ID

Zitieren

Stracke R, De Vos RCH, Bartelniewoehner L, et al. Metabolomic and genetic analyses of flavonol synthesis in Arabidopsis thaliana support the in vivo involvement of leucoanthocyanidin dioxygenase. Planta. 2009;229(2):427-445.
Stracke, R., De Vos, R. C. H., Bartelniewoehner, L., Ishihara, H., Sagasser, M., Martens, S., & Weisshaar, B. (2009). Metabolomic and genetic analyses of flavonol synthesis in Arabidopsis thaliana support the in vivo involvement of leucoanthocyanidin dioxygenase. Planta, 229(2), 427-445. doi:10.1007/s00425-008-0841-y
Stracke, R., De Vos, R. C. H., Bartelniewoehner, L., Ishihara, H., Sagasser, M., Martens, S., and Weisshaar, B. (2009). Metabolomic and genetic analyses of flavonol synthesis in Arabidopsis thaliana support the in vivo involvement of leucoanthocyanidin dioxygenase. Planta 229, 427-445.
Stracke, R., et al., 2009. Metabolomic and genetic analyses of flavonol synthesis in Arabidopsis thaliana support the in vivo involvement of leucoanthocyanidin dioxygenase. Planta, 229(2), p 427-445.
R. Stracke, et al., “Metabolomic and genetic analyses of flavonol synthesis in Arabidopsis thaliana support the in vivo involvement of leucoanthocyanidin dioxygenase”, Planta, vol. 229, 2009, pp. 427-445.
Stracke, R., De Vos, R.C.H., Bartelniewoehner, L., Ishihara, H., Sagasser, M., Martens, S., Weisshaar, B.: Metabolomic and genetic analyses of flavonol synthesis in Arabidopsis thaliana support the in vivo involvement of leucoanthocyanidin dioxygenase. Planta. 229, 427-445 (2009).
Stracke, Ralf, De Vos, Ric C. H., Bartelniewoehner, Lutz, Ishihara, Hirofumi, Sagasser, Martin, Martens, Stefan, and Weisshaar, Bernd. “Metabolomic and genetic analyses of flavonol synthesis in Arabidopsis thaliana support the in vivo involvement of leucoanthocyanidin dioxygenase”. Planta 229.2 (2009): 427-445.

37 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Coloring genetically modified soybean grains with anthocyanins by suppression of the proanthocyanidin genes ANR1 and ANR2.
Kovinich N, Saleem A, Rintoul TL, Brown DC, Arnason JT, Miki B., Transgenic Res 21(4), 2012
PMID: 22083247
Negative regulation of anthocyanin biosynthesis in Arabidopsis by a miR156-targeted SPL transcription factor.
Gou JY, Felippes FF, Liu CJ, Weigel D, Wang JW., Plant Cell 23(4), 2011
PMID: 21487097
Cloning and characterization of a UV-B-inducible maize flavonol synthase.
Falcone Ferreyra ML, Rius S, Emiliani J, Pourcel L, Feller A, Morohashi K, Casati P, Grotewold E., Plant J 62(1), 2010
PMID: 20059741
HvNax3--a locus controlling shoot sodium exclusion derived from wild barley (Hordeum vulgare ssp. spontaneum).
Shavrukov Y, Gupta NK, Miyazaki J, Baho MN, Chalmers KJ, Tester M, Langridge P, Collins NC., Funct Integr Genomics 10(2), 2010
PMID: 20076983
The molecular and physiological responses of Physcomitrella patens to ultraviolet-B radiation.
Wolf L, Rizzini L, Stracke R, Ulm R, Rensing SA., Plant Physiol 153(3), 2010
PMID: 20427465
Developmental, genetic and environmental factors affect the expression of flavonoid genes, enzymes and metabolites in strawberry fruits.
Carbone F, Preuss A, De Vos RC, D'Amico E, Perrotta G, Bovy AG, Martens S, Rosati C., Plant Cell Environ 32(8), 2009
PMID: 19422609
Arabidopsis thaliana expresses a second functional flavonol synthase.
Preuss A, Stracke R, Weisshaar B, Hillebrecht A, Matern U, Martens S., FEBS Lett 583(12), 2009
PMID: 19433090

63 References

Daten bereitgestellt von Europe PubMed Central.


R, Br Z Naturforsch C 39(), 1984
A novel approach for nontargeted data analysis for metabolomics. Large-scale profiling of tomato fruit volatiles.
Tikunov Y, Lommen A, de Vos CH, Verhoeven HA, Bino RJ, Hall RD, Bovy AG., Plant Physiol. 139(3), 2005
PMID: 16286451
Functional genomics by integrated analysis of metabolome and transcriptome of Arabidopsis plants over-expressing an MYB transcription factor.
Tohge T, Nishiyama Y, Hirai MY, Yano M, Nakajima J, Awazuhara M, Inoue E, Takahashi H, Goodenowe DB, Kitayama M, Noji M, Yamazaki M, Saito K., Plant J. 42(2), 2005
PMID: 15807784
The C-4 stereochemistry of leucocyanidin substrates for anthocyanidin synthase affects product selectivity.
Turnbull JJ, Nagle MJ, Seibel JF, Welford RW, Grant GH, Schofield CJ., Bioorg. Med. Chem. Lett. 13(21), 2003
PMID: 14552794
Regulation of flavonol biosynthesis during anther and pistil development, and during pollen tube growth in Solanum tuberosum.
van Eldik GJ, Reijnen WH, Ruiter RK, van Herpen MM, Schrauwen JA, Wullems GJ., Plant J. 11(1), 1997
PMID: 9025306

AUTHOR UNKNOWN, 0
Functional expression and mutational analysis of flavonol synthase from Citrus unshiu.
Wellmann F, Lukacin R, Moriguchi T, Britsch L, Schiltz E, Matern U., Eur. J. Biochem. 269(16), 2002
PMID: 12180990
Knock-out mutants from an En-1 mutagenized Arabidopsis thaliana population generate phenylpropanoid biosynthesis phenotypes.
Wisman E, Hartmann U, Sagasser M, Baumann E, Palme K, Hahlbrock K, Saedler H, Weisshaar B., Proc. Natl. Acad. Sci. U.S.A. 95(21), 1998
PMID: 9770503
Metabolic engineering of anthocyanin biosynthesis in Escherichia coli.
Yan Y, Chemler J, Huang L, Martens S, Koffas MA., Appl. Environ. Microbiol. 71(7), 2005
PMID: 16000769

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 18998159
PubMed | Europe PMC

Suchen in

Google Scholar