Functional characterisation of the peroxiredoxin gene family members of Synechococcus elongatus PCC 7942

Stork T, Laxa M, Dietz MS, Dietz K-J (2009)
ARCHIVES OF MICROBIOLOGY 191(2): 141-151.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
The genome of Synechococcus elongatus PCC 7942 encodes six peroxiredoxins (Prx). Single genes are present each for a 1-Cys Prx and a 2-Cys Prx, while four genes code for PrxQ-like proteins (prxQ-A1, -A2, -A3 and B). Their transcript accumulation varies with growth conditions in a gene-specific manner (Stork et al. in J Exp Bot 56:3193-3206, 2005). To address their functional properties, members of the prx gene family were produced as recombinant proteins and analysed for their peroxide detoxification capacity and quaternary structure by size exclusion chromatography. Independent of the reduction state, the 2-Cys Prx separated as oligomer, the 1-Cys Prx as dimer and the PrxQ-A1 as monomer. PrxQ-A2 was inactive in our assays, 1-Cys Prx activity was unaffected by addition of TrxA, while all others were stimulated to a variable extent by addition of E. coli thioredoxin. Sensitivity towards cumene hydroperoxide treatment of E. coli BL21 cells expressing the cyanobacterial PrxQ-A1 to A3 proteins was greatly reduced, while expression of the other Prx had no effect. The study shows differentiation of Prx functions in S. elongatus PCC 7942 which is discussed in relation to potential roles in site- and stress-specific defence.
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Zeitschriftentitel
ARCHIVES OF MICROBIOLOGY
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191
Zeitschriftennummer
2
Seite
141-151
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Stork T, Laxa M, Dietz MS, Dietz K-J. Functional characterisation of the peroxiredoxin gene family members of Synechococcus elongatus PCC 7942. ARCHIVES OF MICROBIOLOGY. 2009;191(2):141-151.
Stork, T., Laxa, M., Dietz, M. S., & Dietz, K. - J. (2009). Functional characterisation of the peroxiredoxin gene family members of Synechococcus elongatus PCC 7942. ARCHIVES OF MICROBIOLOGY, 191(2), 141-151. doi:10.1007/s00203-008-0438-7
Stork, T., Laxa, M., Dietz, M. S., and Dietz, K. - J. (2009). Functional characterisation of the peroxiredoxin gene family members of Synechococcus elongatus PCC 7942. ARCHIVES OF MICROBIOLOGY 191, 141-151.
Stork, T., et al., 2009. Functional characterisation of the peroxiredoxin gene family members of Synechococcus elongatus PCC 7942. ARCHIVES OF MICROBIOLOGY, 191(2), p 141-151.
T. Stork, et al., “Functional characterisation of the peroxiredoxin gene family members of Synechococcus elongatus PCC 7942”, ARCHIVES OF MICROBIOLOGY, vol. 191, 2009, pp. 141-151.
Stork, T., Laxa, M., Dietz, M.S., Dietz, K.-J.: Functional characterisation of the peroxiredoxin gene family members of Synechococcus elongatus PCC 7942. ARCHIVES OF MICROBIOLOGY. 191, 141-151 (2009).
Stork, Tina, Laxa, Miriam, Dietz, Marina S., and Dietz, Karl-Josef. “Functional characterisation of the peroxiredoxin gene family members of Synechococcus elongatus PCC 7942”. ARCHIVES OF MICROBIOLOGY 191.2 (2009): 141-151.

9 Zitationen in Europe PMC

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Characterization of a bacterioferritin comigratory protein family 1-Cys peroxiredoxin from Candidatus Liberibacter asiaticus.
Singh A, Kumar N, Tomar PPS, Bhose S, Ghosh DK, Roy P, Sharma AK., Protoplasma 254(4), 2017
PMID: 27987036
Peroxiredoxins are conserved markers of circadian rhythms.
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PMID: 22622569
The cellular energization state affects peripheral stalk stability of plant vacuolar H+-ATPase and impairs vacuolar acidification.
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PMID: 21474463
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Dietz KJ., Antioxid Redox Signal 15(4), 2011
PMID: 21194355
Structural and biochemical characterization of peroxiredoxin Qbeta from Xylella fastidiosa: catalytic mechanism and high reactivity.
Horta BB, de Oliveira MA, Discola KF, Cussiol JR, Netto LE., J Biol Chem 285(21), 2010
PMID: 20335172
The oligomeric conformation of peroxiredoxins links redox state to function.
Barranco-Medina S, Lázaro JJ, Dietz KJ., FEBS Lett 583(12), 2009
PMID: 19464293

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