Integrated bioprocess for the production and purification of recombinant proteins by affinity chromatography in Escherichia coli

Beshay U, Miksch G, Friehs K, Flaschel E (2009)
BIOPROCESS AND BIOSYSTEMS ENGINEERING 32(2): 149-158.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
Abstract / Bemerkung
In order to improve the effectiveness of the production of recombinant proteins in E. coli, integrated fermentation processes were developed. Therefore, expression vectors were constructed containing a strongly expressed gene for a beta-glucanase fused with a metal-chelating affinity tag and a leader peptide for directing the fusion protein into the periplasmic space. Its export into the medium was achieved by means of co-expression of a bacteriocin-release protein, the Kil protein from pColE1. Bioreactors were modified so that special devices containing metal chelate pentadentate chelator PDC resins were located within the bioreactor. Using the bioreactor with an internal device the Zn2+-PDC had a 4.3-fold higher binding capacity than metal-free PDC (12.3 and 2.6 kU ml(-1) PDC, respectively. Using the bioreactor with charged PDC in an external circuit revealed even higher beta-glucanase concentration (65.6 kU ml(-1)), i.e. 1.5-fold compared to the internal adsorbent system.
Erscheinungsjahr
Zeitschriftentitel
BIOPROCESS AND BIOSYSTEMS ENGINEERING
Band
32
Ausgabe
2
Seite(n)
149-158
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eISSN
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Beshay U, Miksch G, Friehs K, Flaschel E. Integrated bioprocess for the production and purification of recombinant proteins by affinity chromatography in Escherichia coli. BIOPROCESS AND BIOSYSTEMS ENGINEERING. 2009;32(2):149-158.
Beshay, U., Miksch, G., Friehs, K., & Flaschel, E. (2009). Integrated bioprocess for the production and purification of recombinant proteins by affinity chromatography in Escherichia coli. BIOPROCESS AND BIOSYSTEMS ENGINEERING, 32(2), 149-158. doi:10.1007/s00449-008-0227-3
Beshay, U., Miksch, G., Friehs, K., and Flaschel, E. (2009). Integrated bioprocess for the production and purification of recombinant proteins by affinity chromatography in Escherichia coli. BIOPROCESS AND BIOSYSTEMS ENGINEERING 32, 149-158.
Beshay, U., et al., 2009. Integrated bioprocess for the production and purification of recombinant proteins by affinity chromatography in Escherichia coli. BIOPROCESS AND BIOSYSTEMS ENGINEERING, 32(2), p 149-158.
U. Beshay, et al., “Integrated bioprocess for the production and purification of recombinant proteins by affinity chromatography in Escherichia coli”, BIOPROCESS AND BIOSYSTEMS ENGINEERING, vol. 32, 2009, pp. 149-158.
Beshay, U., Miksch, G., Friehs, K., Flaschel, E.: Integrated bioprocess for the production and purification of recombinant proteins by affinity chromatography in Escherichia coli. BIOPROCESS AND BIOSYSTEMS ENGINEERING. 32, 149-158 (2009).
Beshay, Usama, Miksch, Gerd, Friehs, Karl, and Flaschel, Erwin. “Integrated bioprocess for the production and purification of recombinant proteins by affinity chromatography in Escherichia coli”. BIOPROCESS AND BIOSYSTEMS ENGINEERING 32.2 (2009): 149-158.

4 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Extracellular recombinant protein production from Escherichia coli.
Ni Y, Chen R., Biotechnol Lett 31(11), 2009
PMID: 19597765

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