Microarray studies reveal a 'differential response' to moderate or severe heat shock of the HrcA- and HspR-dependent systems in Corynebacterium glutamicum

Barreiro C, Nakunst D, Hueser AT, de Paz HD, Kalinowski J, Martin JF (2009)
MICROBIOLOGY-SGM 155(2): 359-372.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ;
Abstract
Genome-wide transcription profile analysis of the heat-shocked wild-type strain under moderate (40 degrees C) and severe heat stress (50 degrees C) revealed that a large number of genes are differentially expressed after heat shock. Of these, 358 genes were upregulated and 420 were downregulated in response to moderate heat shock (40 degrees C) in Corynebacterium glutamicum. Our results confirmed the HrcA/controlling inverted repeat of chaperone expression (CIRCE)-dependent and HspR/HspR-associated inverted repeat (HAIR)-dependent upregulation of chaperones following heat shock. Other genes, including clusters of orthologous groups (COG) related to macromolecule biosynthesis and several transcriptional regulators (COG class K), were upregulated, explaining the large number of genes affected by heat shock. Mutants having deletions in the hrcA or hspR regulators were constructed, which allowed the complete identification of the genes controlled by those systems. The up- or downregulation of several genes observed in the microarray experiments was validated by Northern blot analyses and quantitative (real-time) reverse-transcription PCR. These analyses showed a heat-shock intensity-dependent response ('differential response') in the HspR/HAIR system, in contrast to the non-differential response shown by the HrcA/CIRCE-regulated genes.
Publishing Year
ISSN
eISSN
PUB-ID

Cite this

Barreiro C, Nakunst D, Hueser AT, de Paz HD, Kalinowski J, Martin JF. Microarray studies reveal a 'differential response' to moderate or severe heat shock of the HrcA- and HspR-dependent systems in Corynebacterium glutamicum. MICROBIOLOGY-SGM. 2009;155(2):359-372.
Barreiro, C., Nakunst, D., Hueser, A. T., de Paz, H. D., Kalinowski, J., & Martin, J. F. (2009). Microarray studies reveal a 'differential response' to moderate or severe heat shock of the HrcA- and HspR-dependent systems in Corynebacterium glutamicum. MICROBIOLOGY-SGM, 155(2), 359-372.
Barreiro, C., Nakunst, D., Hueser, A. T., de Paz, H. D., Kalinowski, J., and Martin, J. F. (2009). Microarray studies reveal a 'differential response' to moderate or severe heat shock of the HrcA- and HspR-dependent systems in Corynebacterium glutamicum. MICROBIOLOGY-SGM 155, 359-372.
Barreiro, C., et al., 2009. Microarray studies reveal a 'differential response' to moderate or severe heat shock of the HrcA- and HspR-dependent systems in Corynebacterium glutamicum. MICROBIOLOGY-SGM, 155(2), p 359-372.
C. Barreiro, et al., “Microarray studies reveal a 'differential response' to moderate or severe heat shock of the HrcA- and HspR-dependent systems in Corynebacterium glutamicum”, MICROBIOLOGY-SGM, vol. 155, 2009, pp. 359-372.
Barreiro, C., Nakunst, D., Hueser, A.T., de Paz, H.D., Kalinowski, J., Martin, J.F.: Microarray studies reveal a 'differential response' to moderate or severe heat shock of the HrcA- and HspR-dependent systems in Corynebacterium glutamicum. MICROBIOLOGY-SGM. 155, 359-372 (2009).
Barreiro, Carlos, Nakunst, Diana, Hueser, Andrea T., de Paz, Hector D., Kalinowski, Jörn, and Martin, Juan F. “Microarray studies reveal a 'differential response' to moderate or severe heat shock of the HrcA- and HspR-dependent systems in Corynebacterium glutamicum”. MICROBIOLOGY-SGM 155.2 (2009): 359-372.
This data publication is cited in the following publications:
This publication cites the following data publications:

10 Citations in Europe PMC

Data provided by Europe PubMed Central.

Sequence determinants of prokaryotic gene expression level under heat stress.
Xiong H, Yang Y, Hu XP, He YM, Ma BG., Gene 551(1), 2014
PMID: 25168890
Proteome response of Corynebacterium glutamicum to high concentration of industrially relevant C₄ and C₅ dicarboxylic acids.
Vasco-Cardenas MF, Banos S, Ramos A, Martin JF, Barreiro C., J Proteomics 85(), 2013
PMID: 23624027
Protein turnover quantification in a multilabeling approach: from data calculation to evaluation.
Trotschel C, Albaum SP, Wolff D, Schroder S, Goesmann A, Nattkemper TW, Poetsch A., Mol. Cell Proteomics 11(8), 2012
PMID: 22493176
Identification of a stress-induced factor of Corynebacterineae that is involved in the regulation of the outer membrane lipid composition.
Meniche X, Labarre C, de Sousa-d'Auria C, Huc E, Laval F, Tropis M, Bayan N, Portevin D, Guilhot C, Daffe M, Houssin C., J. Bacteriol. 191(23), 2009
PMID: 19801408

73 References

Data provided by Europe PubMed Central.

Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring bacterium of the human skin flora.
Tauch A, Kaiser O, Hain T, Goesmann A, Weisshaar B, Albersmeier A, Bekel T, Bischoff N, Brune I, Chakraborty T, Kalinowski J, Meyer F, Rupp O, Schneiker S, Viehoever P, Puhler A., J. Bacteriol. 187(13), 2005
PMID: 15968079
Role of the ssu and seu genes of Corynebacterium glutamicum ATCC 13032 in utilization of sulfonates and sulfonate esters as sulfur sources.
Koch DJ, Ruckert C, Rey DA, Mix A, Puhler A, Kalinowski J., Appl. Environ. Microbiol. 71(10), 2005
PMID: 16204527
Transcriptional analysis of the F0F1 ATPase operon of Corynebacterium glutamicum ATCC 13032 reveals strong induction by alkaline pH.
Barriuso-Iglesias M, Barreiro C, Flechoso F, Martin JF., Microbiology (Reading, Engl.) 152(Pt 1), 2006
PMID: 16385111
The Brevibacterium flavum sigma factor SigB has a role in the environmental stress response.
Halgasova N, Bukovska G, Ugorcakova J, Timko J, Kormanec J., FEMS Microbiol. Lett. 216(1), 2002
PMID: 12423756
Global analysis of heat shock response in Desulfovibrio vulgaris Hildenborough.
Chhabra SR, He Q, Huang KH, Gaucher SP, Alm EJ, He Z, Hadi MZ, Hazen TC, Wall JD, Zhou J, Arkin AP, Singh AK., J. Bacteriol. 188(5), 2006
PMID: 16484192
Transcriptional heat shock response in the smallest known self-replicating cell, Mycoplasma genitalium.
Musatovova O, Dhandayuthapani S, Baseman JB., J. Bacteriol. 188(8), 2006
PMID: 16585746
How high G+C Gram-positive bacteria and in particular bifidobacteria cope with heat stress: protein players and regulators.
Ventura M, Canchaya C, Zhang Z, Bernini V, Fitzgerald GF, van Sinderen D., FEMS Microbiol. Rev. 30(5), 2006
PMID: 16911042
OxyR regulated the expression of two major catalases, KatA and KatB, along with peroxiredoxin, AhpC in Pseudomonas putida.
Hishinuma S, Yuki M, Fujimura M, Fukumori F., Environ. Microbiol. 8(12), 2006
PMID: 17107553
Studies on transformation of Escherichia coli with plasmids.
Hanahan D., J. Mol. Biol. 166(4), 1983
PMID: 6345791
The transcriptional regulatory network of the amino acid producer Corynebacterium glutamicum.
Brinkrolf K, Brune I, Tauch A., J. Biotechnol. 129(2), 2007
PMID: 17227685
Global transcriptome analysis of the heat shock response of Bifidobacterium longum.
Rezzonico E, Lariani S, Barretto C, Cuanoud G, Giliberti G, Delley M, Arigoni F, Pessi G., FEMS Microbiol. Lett. 271(1), 2007
PMID: 17419761
TAXONOMICAL STUDIES ON GLUTAMIC ACID-PRODUCING BACTERIA
ABE, The Journal of General and Applied Microbiology 13(3), 1967

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 19202085
PubMed | Europe PMC

Search this title in

Google Scholar