Redox-Dependent Regulation of the Stress-Induced Zinc-Finger Protein SAP12 in Arabidopsis thaliana

Stroher E, Wang X-J, Roloff N, Klein P, Husemann A, Dietz K-J (2009)
MOLECULAR PLANT 2(2): 357-367.

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Abstract
The stress-associated protein SAP12 belongs to the stress-associated protein (SAP) family with 14 members in Arabidopsis thaliana. SAP12 contains two AN1 zinc fingers and was identified in diagonal 2D redox SDS-PAGE as a protein undergoing major redox-dependent conformational changes. Its transcript was strongly induced under cold and salt stress in a time-dependent manner similar to SAP10, with high levels after 6 h and decreasing levels after 24 and 48 h. The transcript regulation resembled those of the stress marker peroxiredoxin PrxIID at 24 and 48 h. Recombinant SAP12 protein showed redox-dependent changes in quaternary structure as visualized by altered electrophoretic mobility in non-reducing SDS polyacrylamide gel electrophoresis. The oxidized oligomer was reduced by high dithiothreitol concentrations, and also by E. coli thioredoxin TrxA with low dithiothreitol (DTT) concentrations or NADPH plus NADPH-dependent thioredoxin reductase. From Western blots, the SAP12 protein amount was estimated to be in the range of 0.5 ng mu g(-1) leaf protein. SAP12 protein decreased under salt and cold stress. These data suggest a redox state-linked function of SAP12 in plant cells particularly under cold and salt stress.
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Stroher E, Wang X-J, Roloff N, Klein P, Husemann A, Dietz K-J. Redox-Dependent Regulation of the Stress-Induced Zinc-Finger Protein SAP12 in Arabidopsis thaliana. MOLECULAR PLANT. 2009;2(2):357-367.
Stroher, E., Wang, X. - J., Roloff, N., Klein, P., Husemann, A., & Dietz, K. - J. (2009). Redox-Dependent Regulation of the Stress-Induced Zinc-Finger Protein SAP12 in Arabidopsis thaliana. MOLECULAR PLANT, 2(2), 357-367.
Stroher, E., Wang, X. - J., Roloff, N., Klein, P., Husemann, A., and Dietz, K. - J. (2009). Redox-Dependent Regulation of the Stress-Induced Zinc-Finger Protein SAP12 in Arabidopsis thaliana. MOLECULAR PLANT 2, 357-367.
Stroher, E., et al., 2009. Redox-Dependent Regulation of the Stress-Induced Zinc-Finger Protein SAP12 in Arabidopsis thaliana. MOLECULAR PLANT, 2(2), p 357-367.
E. Stroher, et al., “Redox-Dependent Regulation of the Stress-Induced Zinc-Finger Protein SAP12 in Arabidopsis thaliana”, MOLECULAR PLANT, vol. 2, 2009, pp. 357-367.
Stroher, E., Wang, X.-J., Roloff, N., Klein, P., Husemann, A., Dietz, K.-J.: Redox-Dependent Regulation of the Stress-Induced Zinc-Finger Protein SAP12 in Arabidopsis thaliana. MOLECULAR PLANT. 2, 357-367 (2009).
Stroher, Elke, Wang, Xin-Jia, Roloff, Nils, Klein, Peter, Husemann, Arne, and Dietz, Karl-Josef. “Redox-Dependent Regulation of the Stress-Induced Zinc-Finger Protein SAP12 in Arabidopsis thaliana”. MOLECULAR PLANT 2.2 (2009): 357-367.
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PMID: 19995730

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