Low molecular weight plant extract induces metabolic changes and the secretion of extracellular enzymes, but has a negative effect on the expression of the type-III secretion system in Xanthomonas campestris pv. campestris

Watt TF, Vucur M, Baumgarth B, Watt SA, Niehaus K (2009)
Journal of Biotechnology 140(1-2): 59-67.

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Abstract
Xanthomonas campestris pathovar campestris (Xcc) is a plant pathogenic bacterium and as such has to adapt to a variety of environments. During the course of disease, Xcc colonizes the Surface of its host, infects the xylem in the early stages, and develops a fully saprophytic life-style, aided by secreted degradative enzymes, in the late stages. To get some insight into this complex regulation, Xcc was cultivated in the presence of low molecular weight host plant extract (<10 kDa). From this experiments it could be observed, that malate and Sucrose are taken LIP preferably in such an environment. Furthermore, it was demonstrated, that the plant extract has a negative effect on the gene expression of the hrp-gene cluster, although the activator hrpG was induced. Also, the secretion of degradative enzymes was shown to be upregulated. These observations indicate, that a low molecular weight plant extract (<10 kDa) is a sufficient signal to regulate metabolic pathways and the secretion of enzymes relevant for the development of virulence in Xanthomonas, but has a negative effect oil the expression of genes involved in type-III secretion, (C) 2009 Elsevier B.V. All rights reserved.
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Watt TF, Vucur M, Baumgarth B, Watt SA, Niehaus K. Low molecular weight plant extract induces metabolic changes and the secretion of extracellular enzymes, but has a negative effect on the expression of the type-III secretion system in Xanthomonas campestris pv. campestris. Journal of Biotechnology. 2009;140(1-2):59-67.
Watt, T. F., Vucur, M., Baumgarth, B., Watt, S. A., & Niehaus, K. (2009). Low molecular weight plant extract induces metabolic changes and the secretion of extracellular enzymes, but has a negative effect on the expression of the type-III secretion system in Xanthomonas campestris pv. campestris. Journal of Biotechnology, 140(1-2), 59-67.
Watt, T. F., Vucur, M., Baumgarth, B., Watt, S. A., and Niehaus, K. (2009). Low molecular weight plant extract induces metabolic changes and the secretion of extracellular enzymes, but has a negative effect on the expression of the type-III secretion system in Xanthomonas campestris pv. campestris. Journal of Biotechnology 140, 59-67.
Watt, T.F., et al., 2009. Low molecular weight plant extract induces metabolic changes and the secretion of extracellular enzymes, but has a negative effect on the expression of the type-III secretion system in Xanthomonas campestris pv. campestris. Journal of Biotechnology, 140(1-2), p 59-67.
T.F. Watt, et al., “Low molecular weight plant extract induces metabolic changes and the secretion of extracellular enzymes, but has a negative effect on the expression of the type-III secretion system in Xanthomonas campestris pv. campestris”, Journal of Biotechnology, vol. 140, 2009, pp. 59-67.
Watt, T.F., Vucur, M., Baumgarth, B., Watt, S.A., Niehaus, K.: Low molecular weight plant extract induces metabolic changes and the secretion of extracellular enzymes, but has a negative effect on the expression of the type-III secretion system in Xanthomonas campestris pv. campestris. Journal of Biotechnology. 140, 59-67 (2009).
Watt, Tony Francis, Vucur, Mihael, Baumgarth, Birgit, Watt, Steven Alexander, and Niehaus, Karsten. “Low molecular weight plant extract induces metabolic changes and the secretion of extracellular enzymes, but has a negative effect on the expression of the type-III secretion system in Xanthomonas campestris pv. campestris”. Journal of Biotechnology 140.1-2 (2009): 59-67.
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