Low molecular weight plant extract induces metabolic changes and the secretion of extracellular enzymes, but has a negative effect on the expression of the type-III secretion system in Xanthomonas campestris pv. campestris

Watt TF, Vucur M, Baumgarth B, Watt SA, Niehaus K (2009)
Journal of Biotechnology 140(1-2): 59-67.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Xanthomonas campestris pathovar campestris (Xcc) is a plant pathogenic bacterium and as such has to adapt to a variety of environments. During the course of disease, Xcc colonizes the Surface of its host, infects the xylem in the early stages, and develops a fully saprophytic life-style, aided by secreted degradative enzymes, in the late stages. To get some insight into this complex regulation, Xcc was cultivated in the presence of low molecular weight host plant extract (<10 kDa). From this experiments it could be observed, that malate and Sucrose are taken LIP preferably in such an environment. Furthermore, it was demonstrated, that the plant extract has a negative effect on the gene expression of the hrp-gene cluster, although the activator hrpG was induced. Also, the secretion of degradative enzymes was shown to be upregulated. These observations indicate, that a low molecular weight plant extract (<10 kDa) is a sufficient signal to regulate metabolic pathways and the secretion of enzymes relevant for the development of virulence in Xanthomonas, but has a negative effect oil the expression of genes involved in type-III secretion, (C) 2009 Elsevier B.V. All rights reserved.
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Zeitschriftentitel
Journal of Biotechnology
Band
140
Zeitschriftennummer
1-2
Seite
59-67
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Watt TF, Vucur M, Baumgarth B, Watt SA, Niehaus K. Low molecular weight plant extract induces metabolic changes and the secretion of extracellular enzymes, but has a negative effect on the expression of the type-III secretion system in Xanthomonas campestris pv. campestris. Journal of Biotechnology. 2009;140(1-2):59-67.
Watt, T. F., Vucur, M., Baumgarth, B., Watt, S. A., & Niehaus, K. (2009). Low molecular weight plant extract induces metabolic changes and the secretion of extracellular enzymes, but has a negative effect on the expression of the type-III secretion system in Xanthomonas campestris pv. campestris. Journal of Biotechnology, 140(1-2), 59-67. doi:10.1016/j.jbiotec.2008.12.003
Watt, T. F., Vucur, M., Baumgarth, B., Watt, S. A., and Niehaus, K. (2009). Low molecular weight plant extract induces metabolic changes and the secretion of extracellular enzymes, but has a negative effect on the expression of the type-III secretion system in Xanthomonas campestris pv. campestris. Journal of Biotechnology 140, 59-67.
Watt, T.F., et al., 2009. Low molecular weight plant extract induces metabolic changes and the secretion of extracellular enzymes, but has a negative effect on the expression of the type-III secretion system in Xanthomonas campestris pv. campestris. Journal of Biotechnology, 140(1-2), p 59-67.
T.F. Watt, et al., “Low molecular weight plant extract induces metabolic changes and the secretion of extracellular enzymes, but has a negative effect on the expression of the type-III secretion system in Xanthomonas campestris pv. campestris”, Journal of Biotechnology, vol. 140, 2009, pp. 59-67.
Watt, T.F., Vucur, M., Baumgarth, B., Watt, S.A., Niehaus, K.: Low molecular weight plant extract induces metabolic changes and the secretion of extracellular enzymes, but has a negative effect on the expression of the type-III secretion system in Xanthomonas campestris pv. campestris. Journal of Biotechnology. 140, 59-67 (2009).
Watt, Tony Francis, Vucur, Mihael, Baumgarth, Birgit, Watt, Steven Alexander, and Niehaus, Karsten. “Low molecular weight plant extract induces metabolic changes and the secretion of extracellular enzymes, but has a negative effect on the expression of the type-III secretion system in Xanthomonas campestris pv. campestris”. Journal of Biotechnology 140.1-2 (2009): 59-67.

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