Analysis of the secretome of the soybean symbiont Bradyrhizobium japonicum

Hempel J, Zehner S, Goettfert M, Patschkowski T (2009)
JOURNAL OF BIOTECHNOLOGY 140(1-2): 51-58.

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Proteins from the supernatant of Bradyrhizobium japonicum were separated by two-dimensional gel electrophoresis and stained with Coomassie. This revealed more than 100 protein spots. Sixty-eight proteins were identified by mass spectrometry. Thirty-five are predicted to contain an N-terminal signal peptide characteristic for proteins transported by the general secretory pathway. Most of these appear to be substrate-binding proteins of the ABC transported family. Ten proteins were categorized as unclassified conserved or hypothetical. None of the proteins has similarity to proteins transported by a type I secretion system or to autotransporters. Three of the proteins might be located in the outer membrane. The addition of genistein led to changes in the spot pattern of three flagellar proteins and resulted in the identification of the nodulation outer protein Pgl. Moreover, the application of shot-gun mass spectrometry resulted in the first-time identification of NopB. NopH and NopT, which were present only after genistein induction. Replacing genistein with daidzein or coumestrol reduced the amount of the type III-secreted protein GunA2. (C) Elsevier B.V. All rights reserved.
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Hempel J, Zehner S, Goettfert M, Patschkowski T. Analysis of the secretome of the soybean symbiont Bradyrhizobium japonicum. JOURNAL OF BIOTECHNOLOGY. 2009;140(1-2):51-58.
Hempel, J., Zehner, S., Goettfert, M., & Patschkowski, T. (2009). Analysis of the secretome of the soybean symbiont Bradyrhizobium japonicum. JOURNAL OF BIOTECHNOLOGY, 140(1-2), 51-58. doi:10.1016/j.jbiotec.2008.11.002
Hempel, J., Zehner, S., Goettfert, M., and Patschkowski, T. (2009). Analysis of the secretome of the soybean symbiont Bradyrhizobium japonicum. JOURNAL OF BIOTECHNOLOGY 140, 51-58.
Hempel, J., et al., 2009. Analysis of the secretome of the soybean symbiont Bradyrhizobium japonicum. JOURNAL OF BIOTECHNOLOGY, 140(1-2), p 51-58.
J. Hempel, et al., “Analysis of the secretome of the soybean symbiont Bradyrhizobium japonicum”, JOURNAL OF BIOTECHNOLOGY, vol. 140, 2009, pp. 51-58.
Hempel, J., Zehner, S., Goettfert, M., Patschkowski, T.: Analysis of the secretome of the soybean symbiont Bradyrhizobium japonicum. JOURNAL OF BIOTECHNOLOGY. 140, 51-58 (2009).
Hempel, Jana, Zehner, Susanne, Goettfert, Michael, and Patschkowski, Thomas. “Analysis of the secretome of the soybean symbiont Bradyrhizobium japonicum”. JOURNAL OF BIOTECHNOLOGY 140.1-2 (2009): 51-58.
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Perez-Gimenez J, Covelli JM, Lopez MF, Althabegoiti MJ, Ferrer-Navarro M, Mongiardini EJ, Lodeiro AR., Curr. Microbiol. 65(4), 2012
PMID: 22782468
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Xin DW, Liao S, Xie ZP, Hann DR, Steinle L, Boller T, Staehelin C., PLoS Pathog. 8(5), 2012
PMID: 22615567
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56 References

Data provided by Europe PubMed Central.

Identification of genistein-inducible and type III-secreted proteins of Bradyrhizobium japonicum.
Suss C, Hempel J, Zehner S, Krause A, Patschkowski T, Gottfert M., J. Biotechnol. 126(1), 2006
PMID: 16707185
Symbiotic implications of type III protein secretion machinery in Rhizobium.
Viprey V, Del Greco A, Golinowski W, Broughton WJ, Perret X., Mol. Microbiol. 28(6), 1998
PMID: 9680225
Sequence similarity-driven proteomics in organisms with unknown genomes by LC-MS/MS and automated de novo sequencing.
Waridel P, Frank A, Thomas H, Surendranath V, Sunyaev S, Pevzner P, Shevchenko A., Proteomics 7(14), 2007
PMID: 17623296
TtsI regulates symbiotic genes in Rhizobium species NGR234 by binding to tts boxes.
Wassem R, Kobayashi H, Kambara K, Le Quere A, Walker GC, Broughton WJ, Deakin WJ., Mol. Microbiol. 68(3), 2008
PMID: 18363648
Comprehensive analysis of the extracellular proteins from Xanthomonas campestris pv. campestris B100.
Watt SA, Wilke A, Patschkowski T, Niehaus K., Proteomics 5(1), 2005
PMID: 15619296
Expression of the Bradyrhizobium japonicum type III secretion system in legume nodules and analysis of the associated tts box promoter
Zehner, Mol. Plant-Microbe Interact. 21(), 2008

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