Comparative mutagenesis of Escherichia coli strains with different repair deficiencies irradiated with 222-nm and 254-nm ultraviolet light

Clauss M, Grotjohann N (2009)
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS 673(2): 83-86.

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Photoinactivation and reversion to tryptophan prototrophy were studied in four Escherichia coli strains with different repair deficiencies. Cells were irradiated with 222-nm wavelength UV emitted by an excimer lamp and with 254-nm wavelength UV emitted by a low-pressure mercury lamp. Strain DSM 9494 (trp(-) uvrA(+)) turned out to be most resistant while the strain DSM 9495 (trp(-) uvrA(+)), which is defective in nucleotide-excision repair (NER) was most sensitive to both wavelengths. UV-fluence rates for a respective inactivation were twice as high for 222-nm wavelength UV than for 254-nm UV. No clear difference in efficiency of inactivation could be observed between the two wavelengths in strains DSM 9496 (trp(-) uvrA(+) pKM101) and DSM 9497 (trp(-) uvrA(-) pKM101). In general. more revertants were induced by 254-nm wavelength UV,which corroborates the hypothesis that a higher amount of DNA damage was induced by this wavelength than by 222-nm UV, except for DSM 9497 where no clear difference could be observed regarding the number of revertants induced by both wavelengths. This strain DSM 9497 has a high sensitivity to certain oxidative mutagens compared with other strains, which is indicative of formation of reactive oxygen species during irradiation with 222-nm wavelength UV. (C) 2009 Elsevier B.V. All rights reserved.
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Clauss M, Grotjohann N. Comparative mutagenesis of Escherichia coli strains with different repair deficiencies irradiated with 222-nm and 254-nm ultraviolet light. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS. 2009;673(2):83-86.
Clauss, M., & Grotjohann, N. (2009). Comparative mutagenesis of Escherichia coli strains with different repair deficiencies irradiated with 222-nm and 254-nm ultraviolet light. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 673(2), 83-86.
Clauss, M., and Grotjohann, N. (2009). Comparative mutagenesis of Escherichia coli strains with different repair deficiencies irradiated with 222-nm and 254-nm ultraviolet light. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS 673, 83-86.
Clauss, M., & Grotjohann, N., 2009. Comparative mutagenesis of Escherichia coli strains with different repair deficiencies irradiated with 222-nm and 254-nm ultraviolet light. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 673(2), p 83-86.
M. Clauss and N. Grotjohann, “Comparative mutagenesis of Escherichia coli strains with different repair deficiencies irradiated with 222-nm and 254-nm ultraviolet light”, MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, vol. 673, 2009, pp. 83-86.
Clauss, M., Grotjohann, N.: Comparative mutagenesis of Escherichia coli strains with different repair deficiencies irradiated with 222-nm and 254-nm ultraviolet light. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS. 673, 83-86 (2009).
Clauss, Marcus, and Grotjohann, Norbert. “Comparative mutagenesis of Escherichia coli strains with different repair deficiencies irradiated with 222-nm and 254-nm ultraviolet light”. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS 673.2 (2009): 83-86.
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42 References

Data provided by Europe PubMed Central.

Comparison of Salmonella typhimurium TA102 with Escherichia coli WP2 tester strains.
Wilcox P, Naidoo A, Wedd DJ, Gatehouse DG., Mutagenesis 5(3), 1990
PMID: 2200948
Mutagen testing using TRP+ reversion in Escherichia coli.
Green MH, Muriel WJ., Mutat. Res. 38(1), 1976
PMID: 768757
Isolation and characterization of mutants of Escherichia coli deficient in induction of mutations by ultraviolet light
Kato, Mol. Gen. Genet. 156(), 1977
Purified lexA protein is a repressor of the recA and lexA genes.
Little JW, Mount DW, Yanisch-Perron CR., Proc. Natl. Acad. Sci. U.S.A. 78(7), 1981
PMID: 7027255
Effects of plasmids on chromosome metabolism in bacteria.
Chernin LS, Mikoyan VS., Plasmid 6(1), 1981
PMID: 7025055

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