Conformational Changes of Channelrhodopsin-2

Radu I, Bamann C, Nack M, Nagel G, Bamberg E, Heberle J (2009)
Journal of the American Chemical Society 131(21): 7313-7319.

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Channelrhodopsin-2 (ChR2) is a member of the new class of light-gated ion channels which serve as phototaxis receptors in the green alga Chlamydomonas reinhardtii. The protein is employed in optogenetics where neural circuits are optically stimulated under high spatiotemporal control. Despite its rapidly growing use in physiological experiments, the reaction mechanism of ChR2 is poorly understood. Here, we applied vibrational spectroscopy to trace structural changes of ChR2 after light-excitation of the retinal chromophore. FT-IR difference spectra of the various photocycle intermediates revealed that stages of the photoreaction preceding (Pi state) and succeeding (P-4) the conductive state of the channel (P-3) are associated with large conformational changes of the protein backbone as indicate by strong differences in the amide I bands. Critical hydrogen-bonding changes of protonated carboxylic amino acid side chains (D1 56, E90) were detected and discussed with regard to the functional mechanism. We used the C128T mutant where the lifetime Of P-3 is prolonged and applied FT-IR and resonance Raman spectroscopy to study the conductive P-3 state of ChR2. Finally, a mechanistic model is proposed that links the observed structural changes of ChR2 to the changes in the channel's conductance.
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Radu I, Bamann C, Nack M, Nagel G, Bamberg E, Heberle J. Conformational Changes of Channelrhodopsin-2. Journal of the American Chemical Society. 2009;131(21):7313-7319.
Radu, I., Bamann, C., Nack, M., Nagel, G., Bamberg, E., & Heberle, J. (2009). Conformational Changes of Channelrhodopsin-2. Journal of the American Chemical Society, 131(21), 7313-7319. doi:10.1021/ja8084274
Radu, I., Bamann, C., Nack, M., Nagel, G., Bamberg, E., and Heberle, J. (2009). Conformational Changes of Channelrhodopsin-2. Journal of the American Chemical Society 131, 7313-7319.
Radu, I., et al., 2009. Conformational Changes of Channelrhodopsin-2. Journal of the American Chemical Society, 131(21), p 7313-7319.
I. Radu, et al., “Conformational Changes of Channelrhodopsin-2”, Journal of the American Chemical Society, vol. 131, 2009, pp. 7313-7319.
Radu, I., Bamann, C., Nack, M., Nagel, G., Bamberg, E., Heberle, J.: Conformational Changes of Channelrhodopsin-2. Journal of the American Chemical Society. 131, 7313-7319 (2009).
Radu, Ionela, Bamann, Christian, Nack, Melanie, Nagel, Georg, Bamberg, Ernst, and Heberle, Joachim. “Conformational Changes of Channelrhodopsin-2”. Journal of the American Chemical Society 131.21 (2009): 7313-7319.
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PMID: 23376098
QM/MM simulations of vibrational spectra of bacteriorhodopsin and channelrhodopsin-2.
Welke K, Watanabe HC, Wolter T, Gaus M, Elstner M., Phys Chem Chem Phys 15(18), 2013
PMID: 23385325
Light-dark adaptation of channelrhodopsin C128T mutant.
Ritter E, Piwowarski P, Hegemann P, Bartl FJ., J Biol Chem 288(15), 2013
PMID: 23439646
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Lórenz-Fonfría VA, Resler T, Krause N, Nack M, Gossing M, Fischer von Mollard G, Bamann C, Bamberg E, Schlesinger R, Heberle J., Proc Natl Acad Sci U S A 110(14), 2013
PMID: 23509282
Diversity of Chlamydomonas channelrhodopsins.
Hou SY, Govorunova EG, Ntefidou M, Lane CE, Spudich EN, Sineshchekov OA, Spudich JL., Photochem Photobiol 88(1), 2012
PMID: 22044280
Bioinformatic and mutational analysis of channelrhodopsin-2 protein cation-conducting pathway.
Plazzo AP, De Franceschi N, Da Broi F, Zonta F, Sanasi MF, Filippini F, Mongillo M., J Biol Chem 287(7), 2012
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In channelrhodopsin-2 Glu-90 is crucial for ion selectivity and is deprotonated during the photocycle.
Eisenhauer K, Kuhne J, Ritter E, Berndt A, Wolf S, Freier E, Bartl F, Hegemann P, Gerwert K., J Biol Chem 287(9), 2012
PMID: 22219197
Structural model of channelrhodopsin.
Watanabe HC, Welke K, Schneider F, Tsunoda S, Zhang F, Deisseroth K, Hegemann P, Elstner M., J Biol Chem 287(10), 2012
PMID: 22241469
Crystal structure of the channelrhodopsin light-gated cation channel.
Kato HE, Zhang F, Yizhar O, Ramakrishnan C, Nishizawa T, Hirata K, Ito J, Aita Y, Tsukazaki T, Hayashi S, Hegemann P, Maturana AD, Ishitani R, Deisseroth K, Nureki O., Nature 482(7385), 2012
PMID: 22266941
Kinetics of proton release and uptake by channelrhodopsin-2.
Nack M, Radu I, Schultz BJ, Resler T, Schlesinger R, Bondar AN, del Val C, Abbruzzetti S, Viappiani C, Bamann C, Bamberg E, Heberle J., FEBS Lett 586(9), 2012
PMID: 22504075
Tuning the primary reaction of channelrhodopsin-2 by imidazole, pH, and site-specific mutations.
Scholz F, Bamberg E, Bamann C, Wachtveitl J., Biophys J 102(11), 2012
PMID: 22713581
Spatially asymmetric reorganization of inhibition establishes a motion-sensitive circuit.
Yonehara K, Balint K, Noda M, Nagel G, Bamberg E, Roska B., Nature 469(7330), 2011
PMID: 21170022
Optogenetic long-term manipulation of behavior and animal development.
Schultheis C, Liewald JF, Bamberg E, Nagel G, Gottschalk A., PLoS One 6(4), 2011
PMID: 21533086
New channelrhodopsin with a red-shifted spectrum and rapid kinetics from Mesostigma viride.
Govorunova EG, Spudich EN, Lane CE, Sineshchekov OA, Spudich JL., MBio 2(3), 2011
PMID: 21693637
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Bruun S, Naumann H, Kuhlmann U, Schulz C, Stehfest K, Hegemann P, Hildebrandt P., FEBS Lett 585(24), 2011
PMID: 22094167
The microbial opsin family of optogenetic tools.
Zhang F, Vierock J, Yizhar O, Fenno LE, Tsunoda S, Kianianmomeni A, Prigge M, Berndt A, Cushman J, Polle J, Magnuson J, Hegemann P, Deisseroth K., Cell 147(7), 2011
PMID: 22196724
The DC gate in Channelrhodopsin-2: crucial hydrogen bonding interaction between C128 and D156.
Nack M, Radu I, Gossing M, Bamann C, Bamberg E, von Mollard GF, Heberle J., Photochem Photobiol Sci 9(2), 2010
PMID: 20126794
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Radu I, Budyak IL, Hoomann T, Kim YJ, Engelhard M, Labahn J, Büldt G, Heberle J, Schlesinger R., Biophys Chem 150(1-3), 2010
PMID: 20303644
Evolution of the channelrhodopsin photocycle model.
Stehfest K, Hegemann P., Chemphyschem 11(6), 2010
PMID: 20349494
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Hoffmann J, Aslimovska L, Bamann C, Glaubitz C, Bamberg E, Brutschy B., Phys Chem Chem Phys 12(14), 2010
PMID: 20355288
Microbial rhodopsins in the spotlight.
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PMID: 20691581
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Verhoefen MK, Bamann C, Blöcher R, Förster U, Bamberg E, Wachtveitl J., Chemphyschem 11(14), 2010
PMID: 20730849
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PMID: 19668708
The retinal structure of channelrhodopsin-2 assessed by resonance Raman spectroscopy.
Nack M, Radu I, Bamann C, Bamberg E, Heberle J., FEBS Lett 583(22), 2009
PMID: 19854176

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