Characterization of the putative iron sulfur protein IdiC (ORF5) in Synechococcus elongatus PCC 7942

Pietsch D, Staiger D, Pistorius EK, Michel K-P (2007)
Photosynthesis Research 94(1): 91-108.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
; ; ;
Abstract / Bemerkung
The IdiC protein ((i) under bar ron (d) under bar eficiency (i) under bar nduced protein (C) under bar) is encoded by orf5 (now called idiC), which is part of the iron-responsive idiB operon of Synechococcus elongatus PCC 7942. The 20.5 kDa IdiC protein has a putative transmembrane helix and belongs to the thioredoxin (TRX)-like [2Fe-2S] ferredoxin family. IdiC has the highest similarity to the peripheral subunit NuoE of the Escherichia coli NDH-1 complex. IdiC expression increased under iron starvation and also in the late growth phase, representing growth conditions, which favor photosynthetic cyclic and respiratory electron transport over photosynthetic linear electron transport from water to NADP(+). Attempts to insertionally inactivate the idiC gene generated merodiploid mutants with a strongly reduced IdiC content (mutant MuD) but no IdiC-free mutant. Thus, IdiC seems to be an essential protein for the viability of S. elongatus under the used experimental conditions. Comparative analyses of S. elongatus wild type (WT) and mutant MuD showed that under iron limitation in WT and MuD the amount of the reaction center proteins PsbA and PsaA/B was highly reduced. MuD had a lower growth rate, chlorophyll content, and photosynthetic O-2 evolving activity with bicarbonate as electron acceptor than WT. Immunoblot analyses also showed that in MuD, when grown under iron limitation, the amount of the proteins IdiC and IdiB was greatly reduced as compared to WT. As a consequence of the reduction of the transcription factor IdiB, IdiA and IrpA expression were also decreased. In addition, the IsiA protein concentration was lower in MuD than in WT, although the isiA mRNA was equally high in MuD and WT. Another significant difference was the lower expression of the ferredoxin:NADP(+) oxidoreductase in mutant MuD under iron limitation compared to WT. A possible function of the protein IdiC in cyclic electron transport around photosystem I and/or in respiratory electron transport will be discussed.
Erscheinungsjahr
Zeitschriftentitel
Photosynthesis Research
Band
94
Zeitschriftennummer
1
Seite
91-108
ISSN
eISSN
PUB-ID

Zitieren

Pietsch D, Staiger D, Pistorius EK, Michel K-P. Characterization of the putative iron sulfur protein IdiC (ORF5) in Synechococcus elongatus PCC 7942. Photosynthesis Research. 2007;94(1):91-108.
Pietsch, D., Staiger, D., Pistorius, E. K., & Michel, K. - P. (2007). Characterization of the putative iron sulfur protein IdiC (ORF5) in Synechococcus elongatus PCC 7942. Photosynthesis Research, 94(1), 91-108. doi:10.1007/s11120-007-9222-9
Pietsch, D., Staiger, D., Pistorius, E. K., and Michel, K. - P. (2007). Characterization of the putative iron sulfur protein IdiC (ORF5) in Synechococcus elongatus PCC 7942. Photosynthesis Research 94, 91-108.
Pietsch, D., et al., 2007. Characterization of the putative iron sulfur protein IdiC (ORF5) in Synechococcus elongatus PCC 7942. Photosynthesis Research, 94(1), p 91-108.
D. Pietsch, et al., “Characterization of the putative iron sulfur protein IdiC (ORF5) in Synechococcus elongatus PCC 7942”, Photosynthesis Research, vol. 94, 2007, pp. 91-108.
Pietsch, D., Staiger, D., Pistorius, E.K., Michel, K.-P.: Characterization of the putative iron sulfur protein IdiC (ORF5) in Synechococcus elongatus PCC 7942. Photosynthesis Research. 94, 91-108 (2007).
Pietsch, Daniel, Staiger, Dorothee, Pistorius, Elfriede K., and Michel, Klaus-Peter. “Characterization of the putative iron sulfur protein IdiC (ORF5) in Synechococcus elongatus PCC 7942”. Photosynthesis Research 94.1 (2007): 91-108.

3 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

New insights into the function of the iron deficiency-induced protein C from Synechococcus elongatus PCC 7942.
Pietsch D, Bernát G, Kahmann U, Staiger D, Pistorius EK, Michel KP., Photosynth Res 108(2-3), 2011
PMID: 21607697
The mechanism of iron homeostasis in the unicellular cyanobacterium synechocystis sp. PCC 6803 and its relationship to oxidative stress.
Shcolnick S, Summerfield TC, Reytman L, Sherman LA, Keren N., Plant Physiol 150(4), 2009
PMID: 19561120
Transcript profiling reveals new insights into the acclimation of the mesophilic fresh-water cyanobacterium Synechococcus elongatus PCC 7942 to iron starvation.
Nodop A, Pietsch D, Höcker R, Becker A, Pistorius EK, Forchhammer K, Michel KP., Plant Physiol 147(2), 2008
PMID: 18424627

81 References

Daten bereitgestellt von Europe PubMed Central.


J, 2006
Identification of NdhL and Ssl1690 (NdhO) in NDH-1L and NDH-1M complexes of Synechocystis sp. PCC 6803.
Battchikova N, Zhang P, Rudd S, Ogawa T, Aro EM., J. Biol. Chem. 280(4), 2004
PMID: 15548534
Widespread iron limitation of phytoplankton in the south pacific ocean
Behrenfeld MJ, Kolber ZS., Science 283(5403), 1999
PMID: 9933166

S, FEBS Lett 326(), 1993
A giant chlorophyll-protein complex induced by iron deficiency in cyanobacteria.
Boekema EJ, Hifney A, Yakushevska AE, Piotrowski M, Keegstra W, Berry S, Michel KP, Pistorius EK, Kruip J., Nature 412(6848), 2001
PMID: 11507644

U, Proc Natl Acad Sci USA 103(), 2006
Expression of the iron-responsive irpA gene from the cyanobacterium Synechococcus sp strain PCC 7942.
Durham KA, Porta D, McKay RM, Bullerjahn GS., Arch. Microbiol. 179(2), 2002
PMID: 12560991

K, FEMS Microbiol Lett 155(), 1997
The IdiA protein of Synechococcus sp. PCC 7942 functions in protecting the acceptor side of Photosystem II under oxidative stress.
Exss-Sonne P, Tolle J, Bader KP, Pistorius EK, Michel KP., Photosyn. Res. 63(2), 2000
PMID: 16228425
The role of respiration during adaptation of the freshwater cyanobacterium Synechococcus 6311 to salinity.
Fry IV, Huflejt M, Erber WW, Peschek GA, Packer L., Arch. Biochem. Biophys. 244(2), 1986
PMID: 3004347

S, J Plant Physiol 146(), 1995

M, Microbiology 142(), 1996
Genetic engineering of the cyanobacterial chromosome.
Golden SS, Brusslan J, Haselkorn R., Meth. Enzymol. 153(), 1987
PMID: 3123881
Terminal oxidases of cyanobacteria.
Hart SE, Schlarb-Ridley BG, Bendall DS, Howe CJ., Biochem. Soc. Trans. 33(Pt 4), 2005
PMID: 16042609
The chlorophyll-binding protein IsiA is inducible by high light and protects the cyanobacterium Synechocystis PCC6803 from photooxidative stress.
Havaux M, Guedeney G, Hagemann M, Yeremenko N, Matthijs HC, Jeanjean R., FEBS Lett. 579(11), 2005
PMID: 15848160

M, 2000
Towards functional proteomics of membrane protein complexes in Synechocystis sp. PCC 6803.
Herranen M, Battchikova N, Zhang P, Graf A, Sirpio S, Paakkarinen V, Aro EM., Plant Physiol. 134(1), 2004
PMID: 14730074
Iron stress restricts photosynthetic intersystem electron transport in Synechococcus sp. PCC 7942.
Ivanov AG, Park YI, Miskiewicz E, Raven JA, Huner NP, Oquist G., FEBS Lett. 485(2-3), 2000
PMID: 11094162
Iron deficiency in cyanobacteria causes monomerization of photosystem I trimers and reduces the capacity for state transitions and the effective absorption cross section of photosystem I in vivo.
Ivanov AG, Krol M, Sveshnikov D, Selstam E, Sandstrom S, Koochek M, Park YI, Vasil'ev S, Bruce D, Oquist G, Huner NP., Plant Physiol. 141(4), 2006
PMID: 16798943

R, 1999
A photosystem 1 psaFJ-null mutant of the cyanobacterium Synechocystis PCC 6803 expresses the isiAB operon under iron replete conditions.
Jeanjean R, Zuther E, Yeremenko N, Havaux M, Matthijs HC, Hagemann M., FEBS Lett. 549(1-3), 2003
PMID: 12914924
Cyclic electron transport in C3 plants: fact or artefact?
Johnson GN., J. Exp. Bot. 56(411), 2005
PMID: 15647314

AUTHOR UNKNOWN, 0

F, Physiol Plant 96(), 1996
Structure and functional role of supercomplexes of IsiA and Photosystem I in cyanobacterial photosynthesis.
Kouril R, Arteni AA, Lax J, Yeremenko N, D'Haene S, Rogner M, Matthijs HC, Dekker JP, Boekema EJ., FEBS Lett. 579(15), 2005
PMID: 15943969
Characterization of a cyanobacterial iron stress-induced gene similar to psbC.
Laudenbach DE, Straus NA., J. Bacteriol. 170(11), 1988
PMID: 3141374

JEM, Biochim Biophys 1767(), 2007

JH, Nature 331(), 1988

JH, Nature 371(), 1994

HC, Funct Plant Biol 29(), 2002

H, Plant Cell Physiol 35(), 1994

H, Plant Cell Physiol 33(), 1992

KP, Z Naturforsch [C] 47(), 1992

KP, Physiol Plant 119(), 2004

KP, Microbiology 142(), 1996

KP, Microbiology 145(), 1999
PGR5 is involved in cyclic electron flow around photosystem I and is essential for photoprotection in Arabidopsis.
Munekage Y, Hojo M, Meurer J, Endo T, Tasaka M, Shikanai T., Cell 110(3), 2002
PMID: 12176323
Cyclic electron flow around photosystem I is essential for photosynthesis.
Munekage Y, Hashimoto M, Miyake C, Tomizawa K, Endo T, Tasaka M, Shikanai T., Nature 429(6991), 2004
PMID: 15175756
Structural analysis of the photosystem I supercomplex of cyanobacteria induced by iron deficiency.
Nield J, Morris EP, Bibby TS, Barber J., Biochemistry 42(11), 2003
PMID: 12641449
Inorganic carbon acquisition systems in cyanobacteria.
Ogawa T, Kaplan A., Photosyn. Res. 77(2-3), 2003
PMID: 16228369
Two types of functionally distinct NAD(P)H dehydrogenases in Synechocystis sp. strain PCC6803.
Ohkawa H, Pakrasi HB, Ogawa T., J. Biol. Chem. 275(41), 2000
PMID: 10906128

J, 1989
CP43', the isiA gene product, functions as an excitation energy dissipator in the cyanobacterium Synechococcus sp. PCC 7942.
Sandstrom S, Park YI, Oquist G, Gustafsson P., Photochem. Photobiol. 74(3), 2001
PMID: 11594057

AUTHOR UNKNOWN, 0

DP, Z Naturforsch [C] 55(), 2000

NA, 1994

JC, Proc Natl Acad Sci USA 103(), 2006
Localization and function of the IdiA homologue Slr1295 in the cyanobacterium Synechocystis sp. strain PCC 6803.
Tolle J, Michel KP, Kruip J, Kahmann U, Preisfeld A, Pistorius EK., Microbiology (Reading, Engl.) 148(Pt 10), 2002
PMID: 12368463

AUTHOR UNKNOWN, 0
MapA, an iron-regulated, cytoplasmic membrane protein in the cyanobacterium Synechococcus sp. strain PCC7942.
Webb R, Troyan T, Sherman D, Sherman LA., J. Bacteriol. 176(16), 1994
PMID: 8051004
Open reading frame ssr2016 is required for antimycin A-sensitive photosystem I-driven cyclic electron flow in the cyanobacterium Synechocystis sp. PCC 6803.
Yeremenko N, Jeanjean R, Prommeenate P, Krasikov V, Nixon PJ, Vermaas WF, Havaux M, Matthijs HC., Plant Cell Physiol. 46(8), 2005
PMID: 15946981
Ferredoxin:NADP+ oxidoreductase is a subunit of the chloroplast cytochrome b6f complex.
Zhang H, Whitelegge JP, Cramer WA., J. Biol. Chem. 276(41), 2001
PMID: 11483610

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 17690995
PubMed | Europe PMC

Suchen in

Google Scholar