Scattering suppression and confocal detection in multifocal multiphoton microscopy

Martini J, Andresen V, Anselmetti D (2007)
JOURNAL OF BIOMEDICAL OPTICS 12(3).

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Abstract
We have developed a new descanned parallel (32-fold) pinhole and photomultiplier detection array for multifocal multiphoton microscopy that effectively reduces the blurring effect originating from scattered fluorescence photons in strongly scattering biological media. With this method, we achieve a fourfold improvement in photon statistics for detecting ballistic photons and an increase in spatial resolution by 21% in the lateral and 35% in the axial direction compared to single-beam non-descanned multiphoton microscopy. The new detection concept has been applied to plant leaves and pollen grains to verify the improvements in imaging quality. (C) 2007 Society of Photo-Optical Instrumentation Engineers.
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Martini J, Andresen V, Anselmetti D. Scattering suppression and confocal detection in multifocal multiphoton microscopy. JOURNAL OF BIOMEDICAL OPTICS. 2007;12(3).
Martini, J., Andresen, V., & Anselmetti, D. (2007). Scattering suppression and confocal detection in multifocal multiphoton microscopy. JOURNAL OF BIOMEDICAL OPTICS, 12(3).
Martini, J., Andresen, V., and Anselmetti, D. (2007). Scattering suppression and confocal detection in multifocal multiphoton microscopy. JOURNAL OF BIOMEDICAL OPTICS 12.
Martini, J., Andresen, V., & Anselmetti, D., 2007. Scattering suppression and confocal detection in multifocal multiphoton microscopy. JOURNAL OF BIOMEDICAL OPTICS, 12(3).
J. Martini, V. Andresen, and D. Anselmetti, “Scattering suppression and confocal detection in multifocal multiphoton microscopy”, JOURNAL OF BIOMEDICAL OPTICS, vol. 12, 2007.
Martini, J., Andresen, V., Anselmetti, D.: Scattering suppression and confocal detection in multifocal multiphoton microscopy. JOURNAL OF BIOMEDICAL OPTICS. 12, (2007).
Martini, Joerg, Andresen, Volker, and Anselmetti, Dario. “Scattering suppression and confocal detection in multifocal multiphoton microscopy”. JOURNAL OF BIOMEDICAL OPTICS 12.3 (2007).
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2 Citations in Europe PMC

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Non-descanned multifocal multiphoton microscopy with a multianode photomultiplier tube.
Cha JW, Yew EY, Kim D, Subramanian J, Nedivi E, So PT., AIP Adv 5(8), 2015
PMID: 25874160
Two-photon and second harmonic microscopy in clinical and translational cancer research.
Perry SW, Burke RM, Brown EB., Ann Biomed Eng 40(2), 2012
PMID: 22258888

26 References

Data provided by Europe PubMed Central.

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