Scattering suppression and confocal detection in multifocal multiphoton microscopy

Martini J, Andresen V, Anselmetti D (2007)
JOURNAL OF BIOMEDICAL OPTICS 12(3): 034010.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
; ;
Abstract / Bemerkung
We have developed a new descanned parallel (32-fold) pinhole and photomultiplier detection array for multifocal multiphoton microscopy that effectively reduces the blurring effect originating from scattered fluorescence photons in strongly scattering biological media. With this method, we achieve a fourfold improvement in photon statistics for detecting ballistic photons and an increase in spatial resolution by 21% in the lateral and 35% in the axial direction compared to single-beam non-descanned multiphoton microscopy. The new detection concept has been applied to plant leaves and pollen grains to verify the improvements in imaging quality. (C) 2007 Society of Photo-Optical Instrumentation Engineers.
Erscheinungsjahr
Zeitschriftentitel
JOURNAL OF BIOMEDICAL OPTICS
Band
12
Zeitschriftennummer
3
Artikelnummer
034010
ISSN
PUB-ID

Zitieren

Martini J, Andresen V, Anselmetti D. Scattering suppression and confocal detection in multifocal multiphoton microscopy. JOURNAL OF BIOMEDICAL OPTICS. 2007;12(3): 034010.
Martini, J., Andresen, V., & Anselmetti, D. (2007). Scattering suppression and confocal detection in multifocal multiphoton microscopy. JOURNAL OF BIOMEDICAL OPTICS, 12(3), 034010. doi:10.1117/1.2736425
Martini, J., Andresen, V., and Anselmetti, D. (2007). Scattering suppression and confocal detection in multifocal multiphoton microscopy. JOURNAL OF BIOMEDICAL OPTICS 12:034010.
Martini, J., Andresen, V., & Anselmetti, D., 2007. Scattering suppression and confocal detection in multifocal multiphoton microscopy. JOURNAL OF BIOMEDICAL OPTICS, 12(3): 034010.
J. Martini, V. Andresen, and D. Anselmetti, “Scattering suppression and confocal detection in multifocal multiphoton microscopy”, JOURNAL OF BIOMEDICAL OPTICS, vol. 12, 2007, : 034010.
Martini, J., Andresen, V., Anselmetti, D.: Scattering suppression and confocal detection in multifocal multiphoton microscopy. JOURNAL OF BIOMEDICAL OPTICS. 12, : 034010 (2007).
Martini, Joerg, Andresen, Volker, and Anselmetti, Dario. “Scattering suppression and confocal detection in multifocal multiphoton microscopy”. JOURNAL OF BIOMEDICAL OPTICS 12.3 (2007): 034010.

4 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Non-descanned multifocal multiphoton microscopy with a multianode photomultiplier tube.
Cha JW, Yew EY, Kim D, Subramanian J, Nedivi E, So PT., AIP Adv 5(8), 2015
PMID: 25874160
Fiber-based dual-focus time-demultiplexed second harmonic generation microscopy.
Heuke S, Legesse FB, Lorenz A, Pascher T, Akimov D, Jäger M, Schmitt M, Popp J., Opt Lett 40(11), 2015
PMID: 26030543
Two-photon and second harmonic microscopy in clinical and translational cancer research.
Perry SW, Burke RM, Brown EB., Ann Biomed Eng 40(2), 2012
PMID: 22258888
Multifocal multiphoton excitation and time correlated single photon counting detection for 3-D fluorescence lifetime imaging.
Kumar S, Dunsby C, De Beule PA, Owen DM, Anand U, Lanigan PM, Benninger RK, Davis DM, Neil MA, Anand P, Benham C, Naylor A, French PM., Opt Express 15(20), 2007
PMID: 19550524

28 References

Daten bereitgestellt von Europe PubMed Central.

Two-photon laser scanning fluorescence microscopy.
Denk W, Strickler JH, Webb WW., Science 248(4951), 1990
PMID: 2321027
Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation.
Zipfel WR, Williams RM, Christie R, Nikitin AY, Hyman BT, Webb WW., Proc. Natl. Acad. Sci. U.S.A. 100(12), 2003
PMID: 12756303

AUTHOR UNKNOWN, 0
Multifocal multiphoton microscopy.
Bewersdorf J, Pick R, Hell SW., Opt Lett 23(9), 1998
PMID: 18087301

Sacconi, Opt Lett 28(), 2003
High efficiency beam splitter for multifocal multiphoton microscopy.
Nielsen T, Fricke M, Hellweg D, Andresen P., J Microsc 201(Pt 3), 2001
PMID: 11240852
Widefield multiphoton and temporally decorrelated multifocal multiphoton microscopy.
Fittinghoff D, Wiseman P, Squier J., Opt Express 7(8), 2000
PMID: 19407875
In vivo two-photon laser-scanning microscopy of Ca2+ dynamics in visual motion-sensitive neurons.
Kalb J, Nielsen T, Fricke M, Egelhaaf M, Kurtz R., Biochem. Biophys. Res. Commun. 316(2), 2004
PMID: 15020223
Application of multiline two-photon microscopy to functional in vivo imaging.
Kurtz R, Fricke M, Kalb J, Tinnefeld P, Sauer M., J. Neurosci. Methods 151(2), 2006
PMID: 16442636
Maternal oxytocin triggers a transient inhibitory switch in GABA signaling in the fetal brain during delivery.
Tyzio R, Cossart R, Khalilov I, Minlebaev M, Hubner CA, Represa A, Ben-Ari Y, Khazipov R., Science 314(5806), 2006
PMID: 17170309
Spatially localized ballistic two-photon excitation in scattering media.
Szmacinski H, Gryczynski I, Lakowicz JR., Biospectroscopy 4(5), 1998
PMID: 9787906
Deep tissue two-photon microscopy.
Helmchen F, Denk W., Nat. Methods 2(12), 2005
PMID: 16299478

AUTHOR UNKNOWN, 0

AUTHOR UNKNOWN, 0
High-speed, two-photon scanning microscope.
Kim KH, Buehler C, So PT., Appl Opt 38(28), 1999
PMID: 18324120
Video-rate nonlinear microscopy of neuronal membrane dynamics with genetically encoded probes.
Roorda RD, Hohl TM, Toledo-Crow R, Miesenbock G., J. Neurophysiol. 92(1), 2004
PMID: 14999051
Video-rate scanning two-photon excitation fluorescence microscopy and ratio imaging with cameleons.
Fan GY, Fujisaki H, Miyawaki A, Tsay RK, Tsien RY, Ellisman MH., Biophys. J. 76(5), 1999
PMID: 10233058
Effect of a confocal pinhole in two-photon microscopy.
Gauderon R, Lukins PB, Sheppard CJ., Microsc. Res. Tech. 47(3), 1999
PMID: 10544336

AUTHOR UNKNOWN, 0

AUTHOR UNKNOWN, 0
Nonlinear magic: multiphoton microscopy in the biosciences.
Zipfel WR, Williams RM, Webb WW., Nat. Biotechnol. 21(11), 2003
PMID: 14595365

Sheppard, J Opt Soc Am A 4(), 1987

AUTHOR UNKNOWN, 0

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 17614718
PubMed | Europe PMC

Suchen in

Google Scholar