Engineering photosynthetic light capture: impacts on improved solar energy to biomass conversion

Mussgnug JH, Thomas-Hall S, Rupprecht J, Foo A, Klassen V, McDowall A, Schenk PM, Kruse O, Hankamer B (2007)
PLANT BIOTECHNOLOGY JOURNAL 5(6): 802-814.

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Abstract
The main function of the photosynthetic process is to capture solar energy and to store it in the form of chemical 'fuels'. Increasingly, the photosynthetic machinery is being used for the production of biofuels such as bio-ethanol, biodiesel and bio-H-2. Fuel production efficiency is directly dependent on the solar photon capture and conversion efficiency of the system. Green algae (e.g. Chlamydomonas reinhardtii) have evolved genetic strategies to assemble large light-harvesting antenna complexes (LHC) to maximize light capture under low-light conditions, with the downside that under high solar irradiance, most of the absorbed photons are wasted as fluorescence and heat to protect against photodamage. This limits the production process efficiency of mass culture. We applied RNAi technology to down-regulate the entire LHC gene family simultaneously to reduce energy losses by fluorescence and heat. The mutant Stm3LR3 had significantly reduced levels of LHCI and LHCII mRNAs and proteins while chlorophyll and pigment synthesis was functional. The grana were markedly less tightly stacked, consistent with the role of LHCII. Stm3LR3 also exhibited reduced levels of fluorescence, a higher photosynthetic quantum yield and a reduced sensitivity to photoinhibition, resulting in an increased efficiency of cell cultivation under elevated light conditions. Collectively, these properties offer three advantages in terms of algal bioreactor efficiency under natural high-light levels: (i) reduced fluorescence and LHC-dependent heat losses and thus increased photosynthetic efficiencies under high-light conditions; (ii) improved light penetration properties; and (iii) potentially reduced risk of oxidative photodamage of PSII.
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Mussgnug JH, Thomas-Hall S, Rupprecht J, et al. Engineering photosynthetic light capture: impacts on improved solar energy to biomass conversion. PLANT BIOTECHNOLOGY JOURNAL. 2007;5(6):802-814.
Mussgnug, J. H., Thomas-Hall, S., Rupprecht, J., Foo, A., Klassen, V., McDowall, A., Schenk, P. M., et al. (2007). Engineering photosynthetic light capture: impacts on improved solar energy to biomass conversion. PLANT BIOTECHNOLOGY JOURNAL, 5(6), 802-814. doi:10.1111/j.1467-7652.2007.00285.x
Mussgnug, J. H., Thomas-Hall, S., Rupprecht, J., Foo, A., Klassen, V., McDowall, A., Schenk, P. M., Kruse, O., and Hankamer, B. (2007). Engineering photosynthetic light capture: impacts on improved solar energy to biomass conversion. PLANT BIOTECHNOLOGY JOURNAL 5, 802-814.
Mussgnug, J.H., et al., 2007. Engineering photosynthetic light capture: impacts on improved solar energy to biomass conversion. PLANT BIOTECHNOLOGY JOURNAL, 5(6), p 802-814.
J.H. Mussgnug, et al., “Engineering photosynthetic light capture: impacts on improved solar energy to biomass conversion”, PLANT BIOTECHNOLOGY JOURNAL, vol. 5, 2007, pp. 802-814.
Mussgnug, J.H., Thomas-Hall, S., Rupprecht, J., Foo, A., Klassen, V., McDowall, A., Schenk, P.M., Kruse, O., Hankamer, B.: Engineering photosynthetic light capture: impacts on improved solar energy to biomass conversion. PLANT BIOTECHNOLOGY JOURNAL. 5, 802-814 (2007).
Mussgnug, Jan H., Thomas-Hall, Skye, Rupprecht, Jens, Foo, Alexander, Klassen, Viktor, McDowall, Alasdair, Schenk, Peer M., Kruse, Olaf, and Hankamer, Ben. “Engineering photosynthetic light capture: impacts on improved solar energy to biomass conversion”. PLANT BIOTECHNOLOGY JOURNAL 5.6 (2007): 802-814.
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Singh A, Nigam PS, Murphy JD., Bioresour Technol 102(1), 2011
PMID: 20615690
Engineering cyanobacteria to generate high-value products.
Ducat DC, Way JC, Silver PA., Trends Biotechnol 29(2), 2011
PMID: 21211860
Conversion and conservation of light energy in a photosynthetic microbial mat ecosystem.
Al-Najjar MA, de Beer D, Jørgensen BB, Kühl M, Polerecky L., ISME J 4(3), 2010
PMID: 19907503
Placing microalgae on the biofuels priority list: a review of the technological challenges.
Greenwell HC, Laurens LM, Shields RJ, Lovitt RW, Flynn KJ., J R Soc Interface 7(46), 2010
PMID: 20031983
Genetic engineering of algae for enhanced biofuel production.
Radakovits R, Jinkerson RE, Darzins A, Posewitz MC., Eukaryot Cell 9(4), 2010
PMID: 20139239
Microalgal hydrogen production.
Kruse O, Hankamer B., Curr Opin Biotechnol 21(3), 2010
PMID: 20399635
Developments and perspectives of photobioreactors for biofuel production.
Morweiser M, Kruse O, Hankamer B, Posten C., Appl Microbiol Biotechnol 87(4), 2010
PMID: 20535467
Future prospects of microalgal biofuel production systems.
Stephens E, Ross IL, Mussgnug JH, Wagner LD, Borowitzka MA, Posten C, Kruse O, Hankamer B., Trends Plant Sci 15(10), 2010
PMID: 20655798
Plant-derived vaccines and other therapeutics produced in contained systems.
Franconi R, Demurtas OC, Massa S., Expert Rev Vaccines 9(8), 2010
PMID: 20673011
Diatoms in biotechnology: modern tools and applications.
Bozarth A, Maier UG, Zauner S., Appl Microbiol Biotechnol 82(2), 2009
PMID: 19082585
Raising yield potential in wheat.
Reynolds M, Foulkes MJ, Slafer GA, Berry P, Parry MA, Snape JW, Angus WJ., J Exp Bot 60(7), 2009
PMID: 19363203
Closed photo-bioreactors as tools for biofuel production.
Lehr F, Posten C., Curr Opin Biotechnol 20(3), 2009
PMID: 19501503
Energy biotechnology with cyanobacteria.
Angermayr SA, Hellingwerf KJ, Lindblad P, de Mattos MJ., Curr Opin Biotechnol 20(3), 2009
PMID: 19540103
Engineering algae for biohydrogen and biofuel production.
Beer LL, Boyd ES, Peters JW, Posewitz MC., Curr Opin Biotechnol 20(3), 2009
PMID: 19560336
The technology of microalgal culturing.
Eriksen NT., Biotechnol Lett 30(9), 2008
PMID: 18478186
Aquatic phototrophs: efficient alternatives to land-based crops for biofuels.
Dismukes GC, Carrieri D, Bennette N, Ananyev GM, Posewitz MC., Curr Opin Biotechnol 19(3), 2008
PMID: 18539450
Transcriptome for photobiological hydrogen production induced by sulfur deprivation in the green alga Chlamydomonas reinhardtii.
Nguyen AV, Thomas-Hall SR, Malnoë A, Timmins M, Mussgnug JH, Rupprecht J, Kruse O, Hankamer B, Schenk PM., Eukaryot Cell 7(11), 2008
PMID: 18708561
A green light for engineered algae: redirecting metabolism to fuel a biotechnology revolution.
Rosenberg JN, Oyler GA, Wilkinson L, Betenbaugh MJ., Curr Opin Biotechnol 19(5), 2008
PMID: 18725295

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