Biochemical and molecular characterization of the mitochondrial peroxiredoxin PsPrxII F from Pisum sativum

Barranco-Medina S, Krell T, Finkemeier I, Sevilla F, Lazaro J-J, Dietz K-J (2007)
PLANT PHYSIOLOGY AND BIOCHEMISTRY 45(10-11): 729-739.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ;
Abstract
The pea peroxiredoxin homologue PsPrxII F of the Arabidopsis thaliana mitochondrial AtPrxII F was isolated as cDNA and genomic DNA, and characterized in respect to its biochemical and molecular properties. The deduced amino acid sequence contains an N-terminal targeting address for mitochondrial import. Mitochondrial location of PsPrxII F was confirmed by immunocytochemistry. The mature enzyme, without the transit peptide, has a molecular mass of 18.75 kDa, and, at positions 59 and 84, carries the two catalytic cysteinyl residues which are characteristic for this particular Prx subgroup. Activity of site-directed mutagenized C84S-variant lacking the so-called resolving Cys dropped to about 12% of WT Prx while C59S lost its peroxidatic activity completely. Likewise, WT PsPrxII F and C84S-variant but not C59S protected plasmid DNA against strand breakage in a mixed function oxidation assay. WT PrxII F and the variant proteins aggregated to high mass oligomers not yet described for type II Prx. Upon oxidation with hydrogen peroxide PsPrxII F focussed in a series of spots of distinct pI but similar molecular masses in two-dimensional gels indicating different oxidation states of the protein. Using this technique, partial oxidation was also detected in leaf extracts and isolated mitochondria. PsPrxII F mRNA and protein accumulated in cold and heavy metals treated pea plants suggesting a particular function under stress. (C) 2007 Elsevier Masson SAS. All rights reserved.
Publishing Year
ISSN
PUB-ID

Cite this

Barranco-Medina S, Krell T, Finkemeier I, Sevilla F, Lazaro J-J, Dietz K-J. Biochemical and molecular characterization of the mitochondrial peroxiredoxin PsPrxII F from Pisum sativum. PLANT PHYSIOLOGY AND BIOCHEMISTRY. 2007;45(10-11):729-739.
Barranco-Medina, S., Krell, T., Finkemeier, I., Sevilla, F., Lazaro, J. - J., & Dietz, K. - J. (2007). Biochemical and molecular characterization of the mitochondrial peroxiredoxin PsPrxII F from Pisum sativum. PLANT PHYSIOLOGY AND BIOCHEMISTRY, 45(10-11), 729-739.
Barranco-Medina, S., Krell, T., Finkemeier, I., Sevilla, F., Lazaro, J. - J., and Dietz, K. - J. (2007). Biochemical and molecular characterization of the mitochondrial peroxiredoxin PsPrxII F from Pisum sativum. PLANT PHYSIOLOGY AND BIOCHEMISTRY 45, 729-739.
Barranco-Medina, S., et al., 2007. Biochemical and molecular characterization of the mitochondrial peroxiredoxin PsPrxII F from Pisum sativum. PLANT PHYSIOLOGY AND BIOCHEMISTRY, 45(10-11), p 729-739.
S. Barranco-Medina, et al., “Biochemical and molecular characterization of the mitochondrial peroxiredoxin PsPrxII F from Pisum sativum”, PLANT PHYSIOLOGY AND BIOCHEMISTRY, vol. 45, 2007, pp. 729-739.
Barranco-Medina, S., Krell, T., Finkemeier, I., Sevilla, F., Lazaro, J.-J., Dietz, K.-J.: Biochemical and molecular characterization of the mitochondrial peroxiredoxin PsPrxII F from Pisum sativum. PLANT PHYSIOLOGY AND BIOCHEMISTRY. 45, 729-739 (2007).
Barranco-Medina, Sergio, Krell, Tino, Finkemeier, Iris, Sevilla, Francisca, Lazaro, Juan-Jose, and Dietz, Karl-Josef. “Biochemical and molecular characterization of the mitochondrial peroxiredoxin PsPrxII F from Pisum sativum”. PLANT PHYSIOLOGY AND BIOCHEMISTRY 45.10-11 (2007): 729-739.
This data publication is cited in the following publications:
This publication cites the following data publications:

13 Citations in Europe PMC

Data provided by Europe PubMed Central.

Functional and structural changes in plant mitochondrial PrxII F caused by NO.
Camejo D, Ortiz-Espin A, Lazaro JJ, Romero-Puertas MC, Lazaro-Payo A, Sevilla F, Jimenez A., J Proteomics 119(), 2015
PMID: 25682994
The metabolomics of oxidative stress.
Noctor G, Lelarge-Trouverie C, Mhamdi A., Phytochemistry 112(), 2015
PMID: 25306398
Mitochondrial energy and redox signaling in plants.
Schwarzlander M, Finkemeier I., Antioxid. Redox Signal. 18(16), 2013
PMID: 23234467
Salinity-induced changes in S-nitrosylation of pea mitochondrial proteins.
Camejo D, Romero-Puertas Mdel C, Rodriguez-Serrano M, Sandalio LM, Lazaro JJ, Jimenez A, Sevilla F., J Proteomics 79(), 2013
PMID: 23238061
Peroxiredoxins and NADPH-dependent thioredoxin systems in the model legume Lotus japonicus.
Tovar-Mendez A, Matamoros MA, Bustos-Sanmamed P, Dietz KJ, Cejudo FJ, Rouhier N, Sato S, Tabata S, Becana M., Plant Physiol. 156(3), 2011
PMID: 21562331
Proteomic analysis of common bean seed with storage protein deficiency reveals up-regulation of sulfur-rich proteins and starch and raffinose metabolic enzymes, and down-regulation of the secretory pathway.
Marsolais F, Pajak A, Yin F, Taylor M, Gabriel M, Merino DM, Ma V, Kameka A, Vijayan P, Pham H, Huang S, Rivoal J, Bett K, Hernandez-Sebastia C, Liu Q, Bertrand A, Chapman R., J Proteomics 73(8), 2010
PMID: 20353836
Functional analysis and expression characteristics of chloroplastic Prx IIE
Gama F, Brehelin C, Gelhaye E, Meyer Y, Jacquot JP, Rey P, Rouhier N., Physiol Plant 133(3), 2008
PMID: IND44069763
Functional analysis and expression characteristics of chloroplastic Prx IIE.
Gama F, Brehelin C, Gelhaye E, Meyer Y, Jacquot JP, Rey P, Rouhier N., Physiol Plant 133(3), 2008
PMID: 18422870

43 References

Data provided by Europe PubMed Central.

Genotypic variation of the response to cadmium toxicity in Pisum sativum L.
Metwally A, Safronova VI, Belimov AA, Dietz KJ., J. Exp. Bot. 56(409), 2005
PMID: 15533881
The Arabidopsis thaliana sulfiredoxin is a plastidic cysteine-sulfinic acid reductase involved in the photooxidative stress response
Rey, Plant J. 49(), 2007
Peroxiredoxin, a novel family of peroxidases.
Rhee SG, Kang SW, Chang TS, Jeong W, Kim K., IUBMB Life 52(1-2), 2001
PMID: 11795591
Isolation and characterization of a new peroxiredoxin from poplar sieve tubes that uses either glutaredoxin or thioredoxin as a proton donor.
Rouhier N, Gelhaye E, Sautiere PE, Brun A, Laurent P, Tagu D, Gerard J, de Fay E, Meyer Y, Jacquot JP., Plant Physiol. 127(3), 2001
PMID: 11706208
The plant multigenic family of thiol peroxidises
Rouhier, Free Radical Bio. Med. 38(), 2005
Identification of plant glutaredoxin targets.
Rouhier N, Villarejo A, Srivastava M, Gelhaye E, Keech O, Droux M, Finkemeier I, Samuelsson G, Dietz KJ, Jacquot JP, Wingsle G., Antioxid. Redox Signal. 7(7-8), 2005
PMID: 15998247

Sambrook, 1989
Cadmium-induced changes in the growth and oxidative metabolism of pea plants.
Sandalio LM, Dalurzo HC, Gomez M, Romero-Puertas MC, del Rio LA., J. Exp. Bot. 52(364), 2001
PMID: 11604450
Replacement of nitrate by ammonium as N-source increases salt sensitivity of pea plants. I. Ion concentrations in roots and in leaves
Speer, Plant Cell Environ. 17(), 1994
Differential impact of environmental stresses on the pea mitochondrial proteome.
Taylor NL, Heazlewood JL, Day DA, Millar AH., Mol. Cell Proteomics 4(8), 2005
PMID: 15914488
Hepatic microsomal ethanol oxidation. Hydrogen peroxide formation and the role of catalase.
Thurman RG, Ley HG, Scholz R., Eur. J. Biochem. 25(3), 1972
PMID: 4402915
The affinity maturation of anti-4-hydroxy-3-nitrophenylacetyl mouse monoclonal antibody. A calorimetric study of the antigen-antibody interaction.
Torigoe H, Nakayama T, Imazato M, Shimada I, Arata Y, Sarai A., J. Biol. Chem. 270(38), 1995
PMID: 7673199
Differential regulation of superoxide dismutases in plants exposed to environmental stress.
Tsang EW, Bowler C, Herouart D, Van Camp W, Villarroel R, Genetello C, Van Montagu M, Inze D., Plant Cell 3(8), 1991
PMID: 1820818
In vivo characterization of a thioredoxin h target protein defines a new peroxiredoxin family.
Verdoucq L, Vignols F, Jacquot JP, Chartier Y, Meyer Y., J. Biol. Chem. 274(28), 1999
PMID: 10391912
A cysteine-sulfinic acid in peroxiredoxin regulates H2O2-sensing by the antioxidant Pap1 pathway
Vivancos, Proc. Natl. Acad. Sci. USA 21(), 2005
Rapid measurement of binding constants and heats of binding using a new titration calorimeter.
Wiseman T, Williston S, Brandts JF, Lin LN., Anal. Biochem. 179(1), 1989
PMID: 2757186

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 17881238
PubMed | Europe PMC

Search this title in

Google Scholar