HAG2/MYB76 and HAG3/MYB29 exert a specific and coordinated control on the regulation of aliphatic glucosinolate biosynthesis in *Arabidopsis thaliana*

Gigolashvili T, Engqvist M, Yatusevich R, Müller C, Fluegge U-I (2008)
NEW PHYTOLOGIST 177(3): 627-642.

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Abstract
In a previous transactivation screen, two Arabidopsis thaliana R2R3-MYB transcription factors, HAG2/MYB76 and HAG3/MYB29, along with the already characterized HAG1/MYB28, were identified as putative regulators of aliphatic glucosinolate biosynthesis. Molecular and biochemical characterization of HAG2/MYB76 and HAG3/MYB29 functions was performed using transformants with increased or repressed transcript levels. Real-time PCR assays, cotransformation assays and measurements of glucosinolate contents were used to assess the impact of both MYB factors on the steady-state level of glucosinolate biosynthetic genes and accumulation of aliphatic glucosinolates. Both HAG2/MYB76 and HAG3/MYB29 were shown to be positive regulators of aliphatic glucosinolate biosynthesis. Expression of promoter-beta-glucuronidase (GUS) fusions indicated GUS activities in both vegetative and generative organs, with distinct characteristics for each MYB factor. HAG1/MYB28, HAG2/MYB76 and HAG3/MYB29 reciprocally transactivated each other in the control of aliphatic glucosinolate biosynthesis and downregulated the expression of genes involved in the control of indolic glucosinolate biosynthesis, pointing to a reciprocal negative regulation of these two pathways. All three HAG transcription factors exert a coordinated control on aliphatic glucosinolate biosynthesis.
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Gigolashvili T, Engqvist M, Yatusevich R, Müller C, Fluegge U-I. HAG2/MYB76 and HAG3/MYB29 exert a specific and coordinated control on the regulation of aliphatic glucosinolate biosynthesis in *Arabidopsis thaliana*. NEW PHYTOLOGIST. 2008;177(3):627-642.
Gigolashvili, T., Engqvist, M., Yatusevich, R., Müller, C., & Fluegge, U. - I. (2008). HAG2/MYB76 and HAG3/MYB29 exert a specific and coordinated control on the regulation of aliphatic glucosinolate biosynthesis in *Arabidopsis thaliana*. NEW PHYTOLOGIST, 177(3), 627-642.
Gigolashvili, T., Engqvist, M., Yatusevich, R., Müller, C., and Fluegge, U. - I. (2008). HAG2/MYB76 and HAG3/MYB29 exert a specific and coordinated control on the regulation of aliphatic glucosinolate biosynthesis in *Arabidopsis thaliana*. NEW PHYTOLOGIST 177, 627-642.
Gigolashvili, T., et al., 2008. HAG2/MYB76 and HAG3/MYB29 exert a specific and coordinated control on the regulation of aliphatic glucosinolate biosynthesis in *Arabidopsis thaliana*. NEW PHYTOLOGIST, 177(3), p 627-642.
T. Gigolashvili, et al., “HAG2/MYB76 and HAG3/MYB29 exert a specific and coordinated control on the regulation of aliphatic glucosinolate biosynthesis in *Arabidopsis thaliana*”, NEW PHYTOLOGIST, vol. 177, 2008, pp. 627-642.
Gigolashvili, T., Engqvist, M., Yatusevich, R., Müller, C., Fluegge, U.-I.: HAG2/MYB76 and HAG3/MYB29 exert a specific and coordinated control on the regulation of aliphatic glucosinolate biosynthesis in *Arabidopsis thaliana*. NEW PHYTOLOGIST. 177, 627-642 (2008).
Gigolashvili, Tamara, Engqvist, Martin, Yatusevich, Ruslan, Müller, Caroline, and Fluegge, Ulf-Ingo. “HAG2/MYB76 and HAG3/MYB29 exert a specific and coordinated control on the regulation of aliphatic glucosinolate biosynthesis in *Arabidopsis thaliana*”. NEW PHYTOLOGIST 177.3 (2008): 627-642.
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