MANIPULATION OF CORYNEBACTERIUM-GLUTAMICUM BY GENE DISRUPTION AND REPLACEMENT

SCHWARZER A, Pühler A (1991)
BIO-TECHNOLOGY 9(1): 84-87.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
We have developed a system for the genetic manipulation of the amino acid-producing Corynebacterium glutamicum. Gene disruption and replacement were achieved by intoducing, via conjugation, Escherichia coli vector plasmids carrying manipulated C. glutamicum DNA fragments. We obtained stable mutants in which the chromosomal lysA gene, encoding meso-diaminopimelate decarboxylase, was interrupted by a chloramphenicol resistance cartridge, or in which an essential internal part of the lysA gene was deleted. The deletion mutants retain neither antibiotic resistance markers nor vector sequences. This strategy is generally applicable to the construction of industrial strains to be used in fermentation processes.
Erscheinungsjahr
Zeitschriftentitel
BIO-TECHNOLOGY
Band
9
Zeitschriftennummer
1
Seite
84-87
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SCHWARZER A, Pühler A. MANIPULATION OF CORYNEBACTERIUM-GLUTAMICUM BY GENE DISRUPTION AND REPLACEMENT. BIO-TECHNOLOGY. 1991;9(1):84-87.
SCHWARZER, A., & Pühler, A. (1991). MANIPULATION OF CORYNEBACTERIUM-GLUTAMICUM BY GENE DISRUPTION AND REPLACEMENT. BIO-TECHNOLOGY, 9(1), 84-87. doi:10.1038/nbt0191-84
SCHWARZER, A., and Pühler, A. (1991). MANIPULATION OF CORYNEBACTERIUM-GLUTAMICUM BY GENE DISRUPTION AND REPLACEMENT. BIO-TECHNOLOGY 9, 84-87.
SCHWARZER, A., & Pühler, A., 1991. MANIPULATION OF CORYNEBACTERIUM-GLUTAMICUM BY GENE DISRUPTION AND REPLACEMENT. BIO-TECHNOLOGY, 9(1), p 84-87.
A. SCHWARZER and A. Pühler, “MANIPULATION OF CORYNEBACTERIUM-GLUTAMICUM BY GENE DISRUPTION AND REPLACEMENT”, BIO-TECHNOLOGY, vol. 9, 1991, pp. 84-87.
SCHWARZER, A., Pühler, A.: MANIPULATION OF CORYNEBACTERIUM-GLUTAMICUM BY GENE DISRUPTION AND REPLACEMENT. BIO-TECHNOLOGY. 9, 84-87 (1991).
SCHWARZER, A, and Pühler, Alfred. “MANIPULATION OF CORYNEBACTERIUM-GLUTAMICUM BY GENE DISRUPTION AND REPLACEMENT”. BIO-TECHNOLOGY 9.1 (1991): 84-87.

37 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

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26 References

Daten bereitgestellt von Europe PubMed Central.

TAXONOMICAL STUDIES ON GLUTAMIC ACID-PRODUCING BACTERIA
ABE, The Journal of General and Applied Microbiology 13(3), 1967

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