ISOLATION AND CHARACTERIZATION OF TISSUE INHIBITORS OF METALLOPROTEINASES (TIMP-1 AND TIMP-2) FROM HUMAN RHEUMATOID SYNOVIAL-FLUID

OSTHUES A, KNAUPER V, OBERHOFF R, REINKE H, Tschesche H (1992)
FEBS LETTERS 296(1): 16-20.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
; ; ; ;
Abstract / Bemerkung
The tissue inhibitors of metalloproteinases TIMP-1 and TIMP-2 were purified to apparent homogeneity from human rheumatoid synovial fluid (HRSF). The inhibitors were isolated by dissociation of non-covalent gelatinase/TIMP complexes. TIMP-1 migrated as a single polypeptide with M(r) 28 500 on SDS-PAGE, while the M(r) of TIMP-2 was 21 000. The inhibitory activity was stable under heat and acid pH. N-terminal sequence data were obtained for the first 15 residues of both inhibitors and showed identity to the human fibroblast inhibitors TIMP-1 and TIMP-2. This is the first demonstration that TIMP-1 and TIMP-2 can be directly purified from human rheumatoid synovial fluid. The complex formation between the metalloproteinase inhibitors and leucocyte metalloproteinases was shown by immunoblotting.
Erscheinungsjahr
Zeitschriftentitel
FEBS LETTERS
Band
296
Zeitschriftennummer
1
Seite
16-20
ISSN
PUB-ID

Zitieren

OSTHUES A, KNAUPER V, OBERHOFF R, REINKE H, Tschesche H. ISOLATION AND CHARACTERIZATION OF TISSUE INHIBITORS OF METALLOPROTEINASES (TIMP-1 AND TIMP-2) FROM HUMAN RHEUMATOID SYNOVIAL-FLUID. FEBS LETTERS. 1992;296(1):16-20.
OSTHUES, A., KNAUPER, V., OBERHOFF, R., REINKE, H., & Tschesche, H. (1992). ISOLATION AND CHARACTERIZATION OF TISSUE INHIBITORS OF METALLOPROTEINASES (TIMP-1 AND TIMP-2) FROM HUMAN RHEUMATOID SYNOVIAL-FLUID. FEBS LETTERS, 296(1), 16-20. doi:10.1016/0014-5793(92)80393-U
OSTHUES, A., KNAUPER, V., OBERHOFF, R., REINKE, H., and Tschesche, H. (1992). ISOLATION AND CHARACTERIZATION OF TISSUE INHIBITORS OF METALLOPROTEINASES (TIMP-1 AND TIMP-2) FROM HUMAN RHEUMATOID SYNOVIAL-FLUID. FEBS LETTERS 296, 16-20.
OSTHUES, A., et al., 1992. ISOLATION AND CHARACTERIZATION OF TISSUE INHIBITORS OF METALLOPROTEINASES (TIMP-1 AND TIMP-2) FROM HUMAN RHEUMATOID SYNOVIAL-FLUID. FEBS LETTERS, 296(1), p 16-20.
A. OSTHUES, et al., “ISOLATION AND CHARACTERIZATION OF TISSUE INHIBITORS OF METALLOPROTEINASES (TIMP-1 AND TIMP-2) FROM HUMAN RHEUMATOID SYNOVIAL-FLUID”, FEBS LETTERS, vol. 296, 1992, pp. 16-20.
OSTHUES, A., KNAUPER, V., OBERHOFF, R., REINKE, H., Tschesche, H.: ISOLATION AND CHARACTERIZATION OF TISSUE INHIBITORS OF METALLOPROTEINASES (TIMP-1 AND TIMP-2) FROM HUMAN RHEUMATOID SYNOVIAL-FLUID. FEBS LETTERS. 296, 16-20 (1992).
OSTHUES, A, KNAUPER, V, OBERHOFF, R, REINKE, H, and Tschesche, Harald. “ISOLATION AND CHARACTERIZATION OF TISSUE INHIBITORS OF METALLOPROTEINASES (TIMP-1 AND TIMP-2) FROM HUMAN RHEUMATOID SYNOVIAL-FLUID”. FEBS LETTERS 296.1 (1992): 16-20.

9 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Lymphocyte/macrophage interactions: biomaterial surface-dependent cytokine, chemokine, and matrix protein production.
Chang DT, Jones JA, Meyerson H, Colton E, Kwon IK, Matsuda T, Anderson JM., J Biomed Mater Res A 87(3), 2008
PMID: 18200554
High level production of secreted proteins: example of the human tissue inhibitor of metalloproteinases 1.
Crombez L, Marques B, Lenormand JL, Mouz N, Polack B, Trocme C, Toussaint B., Biochem. Biophys. Res. Commun. 337(3), 2005
PMID: 16219294
Proteins of rat serum V: adjuvant arthritis and its modulation by nonsteroidal anti-inflammatory drugs.
Ebrini I, Agnello D, Miller I, Villa P, Fratelli M, Ghezzi P, Gemeiner M, Chan J, Aebersold R, Gianazza E., Electrophoresis 21(11), 2000
PMID: 10892728
Tissue inhibitor of metalloproteinase-2 (TIMP-2) mRNA is constitutively expressed in bovine, human normal, and osteoarthritic articular chondrocytes.
Zafarullah M, Su S, Martel-Pelletier J, DiBattista JA, Costello BG, Stetler-Stevenson WG, Pelletier JP., J. Cell. Biochem. 60(2), 1996
PMID: 8655631
Oncostatin M differentially regulates tissue inhibitors of metalloproteinases TIMP-1 and TIMP-3 gene expression in human synovial lining cells.
Gatsios P, Haubeck HD, Van de Leur E, Frisch W, Apte SS, Greiling H, Heinrich PC, Graeve L., Eur. J. Biochem. 241(1), 1996
PMID: 8898888
Tissue inhibitor of metalloproteinases-2 (TIMP-2) in rat liver cells is increased by lipopolysaccharide and prostaglandin E2.
Roeb E, Rose-John S, Erren A, Edwards DR, Matern S, Graeve L, Heinrich PC., FEBS Lett. 357(1), 1995
PMID: 8001673
The role of adhesion molecules in the pathogenesis of rheumatoid arthritis.
Postigo AA, Garcia-Vicuna R, Laffon A, Sanchez-Madrid F., Autoimmunity 16(1), 1993
PMID: 8136468
Tissue inhibitor of metalloproteinases (TIMP, aka EPA): structure, control of expression and biological functions.
Denhardt DT, Feng B, Edwards DR, Cocuzzi ET, Malyankar UM., Pharmacol. Ther. 59(3), 1993
PMID: 8309995

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 1730286
PubMed | Europe PMC

Suchen in

Google Scholar