ISOLATION AND CHARACTERIZATION OF TISSUE INHIBITORS OF METALLOPROTEINASES (TIMP-1 AND TIMP-2) FROM HUMAN RHEUMATOID SYNOVIAL-FLUID

OSTHUES A, KNAUPER V, OBERHOFF R, REINKE H, Tschesche H (1992)
FEBS LETTERS 296(1): 16-20.

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The tissue inhibitors of metalloproteinases TIMP-1 and TIMP-2 were purified to apparent homogeneity from human rheumatoid synovial fluid (HRSF). The inhibitors were isolated by dissociation of non-covalent gelatinase/TIMP complexes. TIMP-1 migrated as a single polypeptide with M(r) 28 500 on SDS-PAGE, while the M(r) of TIMP-2 was 21 000. The inhibitory activity was stable under heat and acid pH. N-terminal sequence data were obtained for the first 15 residues of both inhibitors and showed identity to the human fibroblast inhibitors TIMP-1 and TIMP-2. This is the first demonstration that TIMP-1 and TIMP-2 can be directly purified from human rheumatoid synovial fluid. The complex formation between the metalloproteinase inhibitors and leucocyte metalloproteinases was shown by immunoblotting.
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OSTHUES A, KNAUPER V, OBERHOFF R, REINKE H, Tschesche H. ISOLATION AND CHARACTERIZATION OF TISSUE INHIBITORS OF METALLOPROTEINASES (TIMP-1 AND TIMP-2) FROM HUMAN RHEUMATOID SYNOVIAL-FLUID. FEBS LETTERS. 1992;296(1):16-20.
OSTHUES, A., KNAUPER, V., OBERHOFF, R., REINKE, H., & Tschesche, H. (1992). ISOLATION AND CHARACTERIZATION OF TISSUE INHIBITORS OF METALLOPROTEINASES (TIMP-1 AND TIMP-2) FROM HUMAN RHEUMATOID SYNOVIAL-FLUID. FEBS LETTERS, 296(1), 16-20.
OSTHUES, A., KNAUPER, V., OBERHOFF, R., REINKE, H., and Tschesche, H. (1992). ISOLATION AND CHARACTERIZATION OF TISSUE INHIBITORS OF METALLOPROTEINASES (TIMP-1 AND TIMP-2) FROM HUMAN RHEUMATOID SYNOVIAL-FLUID. FEBS LETTERS 296, 16-20.
OSTHUES, A., et al., 1992. ISOLATION AND CHARACTERIZATION OF TISSUE INHIBITORS OF METALLOPROTEINASES (TIMP-1 AND TIMP-2) FROM HUMAN RHEUMATOID SYNOVIAL-FLUID. FEBS LETTERS, 296(1), p 16-20.
A. OSTHUES, et al., “ISOLATION AND CHARACTERIZATION OF TISSUE INHIBITORS OF METALLOPROTEINASES (TIMP-1 AND TIMP-2) FROM HUMAN RHEUMATOID SYNOVIAL-FLUID”, FEBS LETTERS, vol. 296, 1992, pp. 16-20.
OSTHUES, A., KNAUPER, V., OBERHOFF, R., REINKE, H., Tschesche, H.: ISOLATION AND CHARACTERIZATION OF TISSUE INHIBITORS OF METALLOPROTEINASES (TIMP-1 AND TIMP-2) FROM HUMAN RHEUMATOID SYNOVIAL-FLUID. FEBS LETTERS. 296, 16-20 (1992).
OSTHUES, A, KNAUPER, V, OBERHOFF, R, REINKE, H, and Tschesche, Harald. “ISOLATION AND CHARACTERIZATION OF TISSUE INHIBITORS OF METALLOPROTEINASES (TIMP-1 AND TIMP-2) FROM HUMAN RHEUMATOID SYNOVIAL-FLUID”. FEBS LETTERS 296.1 (1992): 16-20.
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