ENZYMATIC FRAGMENT SUBSTITUTION AS A TOOL IN PROTEIN DESIGN

Tschesche H, Groeger C, Wenzel H (1991)
In: BIOMEDICA BIOCHIMICA ACTA. 50. AKADEMIE VERLAG GMBH: S175-S180.

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Abstract
An easy and rapid enzymatic method is described which allows replacement of P'-residues in bovine pancreatic trypsin inhibitor. Insertion of Xaa-Arg or Xaa-Lys into a BPTI fragment lacking P1' = Ala16 and P2' = Arg17 was carried out in a "one pot" reaction catalysed by trypsin in the presence of 80% 1,4 butanediol.
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Tschesche H, Groeger C, Wenzel H. ENZYMATIC FRAGMENT SUBSTITUTION AS A TOOL IN PROTEIN DESIGN. In: BIOMEDICA BIOCHIMICA ACTA. Vol 50. AKADEMIE VERLAG GMBH; 1991: S175-S180.
Tschesche, H., Groeger, C., & Wenzel, H. (1991). ENZYMATIC FRAGMENT SUBSTITUTION AS A TOOL IN PROTEIN DESIGN. BIOMEDICA BIOCHIMICA ACTA, 50(10-11), S175-S180.
Tschesche, H., Groeger, C., and Wenzel, H. (1991). “ENZYMATIC FRAGMENT SUBSTITUTION AS A TOOL IN PROTEIN DESIGN” in BIOMEDICA BIOCHIMICA ACTA, vol. 50, (AKADEMIE VERLAG GMBH), S175-S180.
Tschesche, H., Groeger, C., & Wenzel, H., 1991. ENZYMATIC FRAGMENT SUBSTITUTION AS A TOOL IN PROTEIN DESIGN. In BIOMEDICA BIOCHIMICA ACTA. no.50 AKADEMIE VERLAG GMBH, pp. S175-S180.
H. Tschesche, C. Groeger, and H. Wenzel, “ENZYMATIC FRAGMENT SUBSTITUTION AS A TOOL IN PROTEIN DESIGN”, BIOMEDICA BIOCHIMICA ACTA, vol. 50, AKADEMIE VERLAG GMBH, 1991, pp.S175-S180.
Tschesche, H., Groeger, C., Wenzel, H.: ENZYMATIC FRAGMENT SUBSTITUTION AS A TOOL IN PROTEIN DESIGN. BIOMEDICA BIOCHIMICA ACTA. 50, p. S175-S180. AKADEMIE VERLAG GMBH (1991).
Tschesche, Harald, Groeger, C., and Wenzel, Herbert. “ENZYMATIC FRAGMENT SUBSTITUTION AS A TOOL IN PROTEIN DESIGN”. BIOMEDICA BIOCHIMICA ACTA. AKADEMIE VERLAG GMBH, 1991.Vol. 50. S175-S180.
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Recent applications of enzymatic peptide synthesis.
Bongers J, Heimer EP., Peptides 15(1), 1994
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