MEMBERS OF A BROADBEAN NODULIN FAMILY WITH PARTIAL HOMOLOGIES TO THE ALFALFA NODULIN-25 ARE COMPOSED OF 2 TYPES OF AMINO-ACID REPEATS FLANKED BY UNIQUE AMINO-ACID-SEQUENCE TERMINI

Küster H, Perlick AM, Pühler A (1994)
PLANT MOLECULAR BIOLOGY 24(1): 143-157.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ;
Abstract
Five cross-hybridizing cDNAs from clone group 'VfNDS-L' of a broadbean nodule-specific cDNA library differed by five specific in-frame deletions within the coding region. Northern blot analysis revealed that the transcripts represented by these clones were expressed in a nodule-specific way and therefore encoded a new family of broadbean nodulins. These nodulins have been designated Nvf-28/32. The Nvf-28/32 proteins were between 269 and 299 amino acids long with a high proportion of charged amino acids and contained a putative signal peptide of 20 amino acids. Sequence analysis indicated that the central part of the Nvf-28/32 proteins was composed of two different types of repeating amino acid stretches designated 'repeat 1' and 'repeat 2', whereas the N- and C-termini were unique. Tn contrast to 'repeat 1' stretches, which contained both positively and negatively charged amino acid residues, 'repeat 2' sequences did not contain any positively, but a total of 13 negatively charged amino acid residues. We could demonstrate that the five deletions identified exactly corresponded to complete repeats. The unique sequence termini of the Nvf-28/32 proteins displayed strong homologies to the late nodulin 25 from alfalfa. In addition, the repeating units identified were significantly homologous to several, but not all exon parts of this protein. We speculate that the 'VfNDS-L' transcripts were derived from a differential splicing mechanism and that the Nvf-28/32 proteins fulfil a structural rather than an enzymatic
Publishing Year
ISSN
eISSN
PUB-ID

Cite this

Küster H, Perlick AM, Pühler A. MEMBERS OF A BROADBEAN NODULIN FAMILY WITH PARTIAL HOMOLOGIES TO THE ALFALFA NODULIN-25 ARE COMPOSED OF 2 TYPES OF AMINO-ACID REPEATS FLANKED BY UNIQUE AMINO-ACID-SEQUENCE TERMINI. PLANT MOLECULAR BIOLOGY. 1994;24(1):143-157.
Küster, H., Perlick, A. M., & Pühler, A. (1994). MEMBERS OF A BROADBEAN NODULIN FAMILY WITH PARTIAL HOMOLOGIES TO THE ALFALFA NODULIN-25 ARE COMPOSED OF 2 TYPES OF AMINO-ACID REPEATS FLANKED BY UNIQUE AMINO-ACID-SEQUENCE TERMINI. PLANT MOLECULAR BIOLOGY, 24(1), 143-157.
Küster, H., Perlick, A. M., and Pühler, A. (1994). MEMBERS OF A BROADBEAN NODULIN FAMILY WITH PARTIAL HOMOLOGIES TO THE ALFALFA NODULIN-25 ARE COMPOSED OF 2 TYPES OF AMINO-ACID REPEATS FLANKED BY UNIQUE AMINO-ACID-SEQUENCE TERMINI. PLANT MOLECULAR BIOLOGY 24, 143-157.
Küster, H., Perlick, A.M., & Pühler, A., 1994. MEMBERS OF A BROADBEAN NODULIN FAMILY WITH PARTIAL HOMOLOGIES TO THE ALFALFA NODULIN-25 ARE COMPOSED OF 2 TYPES OF AMINO-ACID REPEATS FLANKED BY UNIQUE AMINO-ACID-SEQUENCE TERMINI. PLANT MOLECULAR BIOLOGY, 24(1), p 143-157.
H. Küster, A.M. Perlick, and A. Pühler, “MEMBERS OF A BROADBEAN NODULIN FAMILY WITH PARTIAL HOMOLOGIES TO THE ALFALFA NODULIN-25 ARE COMPOSED OF 2 TYPES OF AMINO-ACID REPEATS FLANKED BY UNIQUE AMINO-ACID-SEQUENCE TERMINI”, PLANT MOLECULAR BIOLOGY, vol. 24, 1994, pp. 143-157.
Küster, H., Perlick, A.M., Pühler, A.: MEMBERS OF A BROADBEAN NODULIN FAMILY WITH PARTIAL HOMOLOGIES TO THE ALFALFA NODULIN-25 ARE COMPOSED OF 2 TYPES OF AMINO-ACID REPEATS FLANKED BY UNIQUE AMINO-ACID-SEQUENCE TERMINI. PLANT MOLECULAR BIOLOGY. 24, 143-157 (1994).
Küster, Helge, Perlick, A. M., and Pühler, Alfred. “MEMBERS OF A BROADBEAN NODULIN FAMILY WITH PARTIAL HOMOLOGIES TO THE ALFALFA NODULIN-25 ARE COMPOSED OF 2 TYPES OF AMINO-ACID REPEATS FLANKED BY UNIQUE AMINO-ACID-SEQUENCE TERMINI”. PLANT MOLECULAR BIOLOGY 24.1 (1994): 143-157.
This data publication is cited in the following publications:
This publication cites the following data publications:

7 Citations in Europe PMC

Data provided by Europe PubMed Central.

The signal peptide of the Medicago truncatula modular nodulin MtNOD25 operates as an address label for the specific targeting of proteins to nitrogen-fixing symbiosomes.
Hohnjec N, Lenz F, Fehlberg V, Vieweg MF, Baier MC, Hause B, Kuster H., Mol. Plant Microbe Interact. 22(1), 2009
PMID: 19061403
Cloning of nodule-specific cDNAs of Galega orientalis.
Kaijalainen S, Schroda M, Lindstrom K., Physiol Plant 114(4), 2002
PMID: 11975733
Expression of genes encoding late nodulins characterized by a putative signal peptide and conserved cysteine residues is reduced in ineffective pea nodules.
Kato T, Kawashima K, Miwa M, Mimura Y, Tamaoki M, Kouchi H, Suganuma N., Mol. Plant Microbe Interact. 15(2), 2002
PMID: 11876425
The Vicia faba leghemoglobin gene VfLb29 is induced in root nodules and in roots colonized by the arbuscular mycorrhizal fungus Glomus fasciculatum.
Fruhling M, Roussel H, Gianinazzi-Pearson V, Puhler A, Perlick AM., Mol. Plant Microbe Interact. 10(1), 1997
PMID: 9002275

34 References

Data provided by Europe PubMed Central.


R, Nucl Acids Res 10(), 1982

R, Nucl Acids Res 14(), 1986
Signal sequences. The limits of variation.
von Heijne G., J. Mol. Biol. 184(1), 1985
PMID: 4032478

G, Nucl Acids Res 14(), 1986

A, Plant Mol Biol Rep 2(), 1984

DPS, 1988

DPS, Physiol Plant 85(), 1992

J, Nucl Acids Res 18(), 1990

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 8111013
PubMed | Europe PMC

Search this title in

Google Scholar