A 4.6 KB DNA REGION OF RHIZOBIUM-MELILOTI INVOLVED IN DETERMINING UREASE AND HYDROGENASE ACTIVITIES CARRIES THE STRUCTURAL GENES FOR UREASE (UREA, UREB, UREC) INTERRUPTED BY OTHER OPEN READING FRAMES

Miksch G, Arnold W, LENTZSCH P, PRIEFER UB, Pühler A (1994)
MOLECULAR & GENERAL GENETICS 242(5): 539-550.

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A 4.6 kb DNA region of the Rhizobium meliloti strain AK631 was found to contain seven open reading frames (ORFs), all oriented in the same direction. The putative gene products of four of these ORFs were highly homologous to UreA, UreB and UreC of Klebsiella aerogenes, Proteus mirabilis, Proteus vulgaris and Canavalia ensiformis. The overall organisation of the DNA region analysed was ORF1, ureA (ORF 2), ORF3, ureB (ORF4), ORF5, ORF6 and ureC (ORF7), indicating that the organisation of the urease structural genes in R. meliloti differs from that of other urease genes so far characterized. ORF1 was incomplete; only the 3' end of the coding region was present. The six complete ORFs coded for polypeptides of 11.1 (UreA), 8.9 (ORF3), 10.8 (UreB), 15.0 (ORF5), 13.8 (ORF6) and 60.7 kDa (UreC). No sequence homology to known polypeptides could be detected for the gene products of ORF1, ORF3, ORF5 and ORF6. Using a lacZ fusion and insertional mutagenesis it was shown that the seven ORFs identified were all located in the same transcription unit. For mutational analysis a resistance gene cassette was introduced into each of the complete ORFs resulting in apolar mutations. Mutations in ureA, ureB and ureC, but not in ORF3, ORF5 and ORF6, abolished urease activity in R. meliloti. The determination of hydrogen uptake in these R. meliloti mutants revealed that only ORF6 and ureB are necessary for hydrogen uptake.
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Miksch G, Arnold W, LENTZSCH P, PRIEFER UB, Pühler A. A 4.6 KB DNA REGION OF RHIZOBIUM-MELILOTI INVOLVED IN DETERMINING UREASE AND HYDROGENASE ACTIVITIES CARRIES THE STRUCTURAL GENES FOR UREASE (UREA, UREB, UREC) INTERRUPTED BY OTHER OPEN READING FRAMES. MOLECULAR & GENERAL GENETICS. 1994;242(5):539-550.
Miksch, G., Arnold, W., LENTZSCH, P., PRIEFER, U. B., & Pühler, A. (1994). A 4.6 KB DNA REGION OF RHIZOBIUM-MELILOTI INVOLVED IN DETERMINING UREASE AND HYDROGENASE ACTIVITIES CARRIES THE STRUCTURAL GENES FOR UREASE (UREA, UREB, UREC) INTERRUPTED BY OTHER OPEN READING FRAMES. MOLECULAR & GENERAL GENETICS, 242(5), 539-550.
Miksch, G., Arnold, W., LENTZSCH, P., PRIEFER, U. B., and Pühler, A. (1994). A 4.6 KB DNA REGION OF RHIZOBIUM-MELILOTI INVOLVED IN DETERMINING UREASE AND HYDROGENASE ACTIVITIES CARRIES THE STRUCTURAL GENES FOR UREASE (UREA, UREB, UREC) INTERRUPTED BY OTHER OPEN READING FRAMES. MOLECULAR & GENERAL GENETICS 242, 539-550.
Miksch, G., et al., 1994. A 4.6 KB DNA REGION OF RHIZOBIUM-MELILOTI INVOLVED IN DETERMINING UREASE AND HYDROGENASE ACTIVITIES CARRIES THE STRUCTURAL GENES FOR UREASE (UREA, UREB, UREC) INTERRUPTED BY OTHER OPEN READING FRAMES. MOLECULAR & GENERAL GENETICS, 242(5), p 539-550.
G. Miksch, et al., “A 4.6 KB DNA REGION OF RHIZOBIUM-MELILOTI INVOLVED IN DETERMINING UREASE AND HYDROGENASE ACTIVITIES CARRIES THE STRUCTURAL GENES FOR UREASE (UREA, UREB, UREC) INTERRUPTED BY OTHER OPEN READING FRAMES”, MOLECULAR & GENERAL GENETICS, vol. 242, 1994, pp. 539-550.
Miksch, G., Arnold, W., LENTZSCH, P., PRIEFER, U.B., Pühler, A.: A 4.6 KB DNA REGION OF RHIZOBIUM-MELILOTI INVOLVED IN DETERMINING UREASE AND HYDROGENASE ACTIVITIES CARRIES THE STRUCTURAL GENES FOR UREASE (UREA, UREB, UREC) INTERRUPTED BY OTHER OPEN READING FRAMES. MOLECULAR & GENERAL GENETICS. 242, 539-550 (1994).
Miksch, Gerd, Arnold, Walter, LENTZSCH, P, PRIEFER, UB, and Pühler, Alfred. “A 4.6 KB DNA REGION OF RHIZOBIUM-MELILOTI INVOLVED IN DETERMINING UREASE AND HYDROGENASE ACTIVITIES CARRIES THE STRUCTURAL GENES FOR UREASE (UREA, UREB, UREC) INTERRUPTED BY OTHER OPEN READING FRAMES”. MOLECULAR & GENERAL GENETICS 242.5 (1994): 539-550.
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Identification of the nik gene cluster of Brucella suis: regulation and contribution to urease activity.
Jubier-Maurin V, Rodrigue A, Ouahrani-Bettache S, Layssac M, Mandrand-Berthelot MA, Kohler S, Liautard JP., J. Bacteriol. 183(2), 2001
PMID: 11133934
Characterisation of the urease gene cluster in Bordetella bronchiseptica.
McMillan DJ, Mau M, Walker MJ., Gene 208(2), 1998
PMID: 9524276
Clostridium perfringens urease genes are plasmid borne.
Dupuy B, Daube G, Popoff MR, Cole ST., Infect. Immun. 65(6), 1997
PMID: 9169769
Molecular biology of microbial ureases.
Mobley HL, Island MD, Hausinger RP., Microbiol. Rev. 59(3), 1995
PMID: 7565414

53 References

Data provided by Europe PubMed Central.

Multiple proteins encoded within the urease gene complex of Proteus mirabilis.
Walz SE, Wray SK, Hull SI, Hull RA., J. Bacteriol. 170(3), 1988
PMID: 2830226
On the evolution of Tn21-like multiresistance transposons: sequence analysis of the gene (aacC1) for gentamicin acetyltransferase-3-I(AAC(3)-I), another member of the Tn21-based expression cassette.
Wohlleben W, Arnold W, Bissonnette L, Pelletier A, Tanguay A, Roy PH, Gamboa GC, Barry GF, Aubert E, Davies J., Mol. Gen. Genet. 217(2-3), 1989
PMID: 2549372

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