The new class II transposon Tn163 is plasmid-borne in two unrelated Rhizobium leguminosarum biovar viciae strains

Ulrich A, Pühler A (1994)
Molecular & General Genetics 242(5): 505-516.

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Tn163 is a transposable element identified in Rhizobium leguminosarum bv. viciae by its high insertion rate into positive selection vectors. The 4.6 kb element was found in only one further R. leguminosarum bv. viciae strain out of 70 strains investigated. Both unrelated R. leguminosarum bv. viciae strains contained one copy of the transposable element, which was localized in plasmids native to these strains. DNA sequence analysis revealed three large open reading frames (ORFs) and 38 bp terminal inverted repeats. ORF1 encodes a putative protein of 990 amino acids displaying strong homologies to transposases of class II transposons. ORF2, transcribed in the opposite direction, codes for a protein of 213 amino acids which is highly homologous to DNA invertases and resolvases of class II transposons. Homology of ORF1 and ORF2 and the genetic structure of the element indicate that Tn163 can be classified as a class II transposon. It is the first example of a native transposon in the genus Rhizobium. ORF3, which was found not to be involved in the transposition process, encodes a putative protein (256 amino acids) of unknown function. During transposition Tn163 produced direct repeats of 5 bp, which is typical for transposons of the Tn3 family. However, one out of the ten insertion sites sequenced showed a 6 bp duplication of the target DNA; all duplicated sequences were A/T rich. Insertion of Tn163 into the sacB gene revealed two hot spots. Chromosomes of different R. leguminosarum bv. viciae strains were found to be highly refractory to the insertion of Tn163.
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Ulrich A, Pühler A. The new class II transposon Tn163 is plasmid-borne in two unrelated Rhizobium leguminosarum biovar viciae strains. Molecular & General Genetics. 1994;242(5):505-516.
Ulrich, A., & Pühler, A. (1994). The new class II transposon Tn163 is plasmid-borne in two unrelated Rhizobium leguminosarum biovar viciae strains. Molecular & General Genetics, 242(5), 505-516.
Ulrich, A., and Pühler, A. (1994). The new class II transposon Tn163 is plasmid-borne in two unrelated Rhizobium leguminosarum biovar viciae strains. Molecular & General Genetics 242, 505-516.
Ulrich, A., & Pühler, A., 1994. The new class II transposon Tn163 is plasmid-borne in two unrelated Rhizobium leguminosarum biovar viciae strains. Molecular & General Genetics, 242(5), p 505-516.
A. Ulrich and A. Pühler, “The new class II transposon Tn163 is plasmid-borne in two unrelated Rhizobium leguminosarum biovar viciae strains”, Molecular & General Genetics, vol. 242, 1994, pp. 505-516.
Ulrich, A., Pühler, A.: The new class II transposon Tn163 is plasmid-borne in two unrelated Rhizobium leguminosarum biovar viciae strains. Molecular & General Genetics. 242, 505-516 (1994).
Ulrich, A., and Pühler, Alfred. “The new class II transposon Tn163 is plasmid-borne in two unrelated Rhizobium leguminosarum biovar viciae strains”. Molecular & General Genetics 242.5 (1994): 505-516.
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Coordination of plastid and nuclear gene expression.
Gray JC, Sullivan JA, Wang JH, Jerome CA, MacLean D., Philos. Trans. R. Soc. Lond., B, Biol. Sci. 358(1429), 2003
PMID: 12594922
Transposon targeting determined by resolvase.
Kamali-Moghaddam M, Sundstrom L., FEMS Microbiol. Lett. 186(1), 2000
PMID: 10779712
ISRf1, a transposable insertion sequence from Sinorhizobium fredii.
Vinardell JM, Ollero FJ, Krishnan HB, del Rosario Espuny M, Villalobo E, Pueppke SG, Ruiz-Sainz JE., Gene 204(1-2), 1997
PMID: 9434166
Structure and evolution of NGRRS-1, a complex, repeated element in the genome of Rhizobium sp. strain NGR234.
Perret X, Viprey V, Freiberg C, Broughton WJ., J. Bacteriol. 179(23), 1997
PMID: 9393715

56 References

Data provided by Europe PubMed Central.

The DNA sequence of the gene for the secreted Bacillus subtilis enzyme levansucrase and its genetic control sites.
Steinmetz M, Le Coq D, Aymerich S, Gonzy-Treboul G, Gay P., Mol. Gen. Genet. 200(2), 1985
PMID: 2993818
Probe mapping to facilitate transposon-based DNA sequencing.
Strausbaugh LD, Bourke MT, Sommer MT, Coon ME, Berg CM., Proc. Natl. Acad. Sci. U.S.A. 87(16), 1990
PMID: 2166950
Toluene transposons Tn4651 and Tn4653 are class II transposons.
Tsuda M, Minegishi K, Iino T., J. Bacteriol. 171(3), 1989
PMID: 2537816

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