TRANSFORMING GROWTH-FACTOR-BETA-1 REGULATES TISSUE INHIBITOR OF METALLOPROTEINASES-1 EXPRESSION IN DIFFERENTIATED HUMAN ARTICULAR CHONDROCYTES

GUNTHER M, HAUBECK HD, VANDELEUR E, BLASER J, BENDER S, GUTGEMANN I, FISCHER DC, Tschesche H, GREILING H, HEINRICH PC, GRAEVE L (1994)
ARTHRITIS AND RHEUMATISM 37(3): 395-405.

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Abstract
Objective. To investigate the role of interleukin-6 (IL-6) and transforming growth factor beta 1 (TGF beta 1) in the regulation of tissue inhibitor of metalloproteinases-1 (TIMP-1) synthesis in human articular chondrocytes. Methods. Articular cartilage was obtained from human knee joints 24 hours after death. Chondrocytes were isolated by collagenase digestion and embedded in low-gelling-temperature agarose. After stimulation by cytokines, total RNA was isolated and analyzed by Northern blotting. TIMP-1 protein levels were determined using a competitive enzyme-linked immunosorbent assay. Results. Human chondrocytes in agarose culture expressed messenger RNA (mRNA) for the IL-6 receptor (gp80) and its signal-transducing subunit gp130. In contrast to the findings in a previous study, IL-6 did not stimulate TIMP-1 expression in these cells, whereas TGF beta 1 was an important inducer of TIMP-1 mRNA and protein synthesis. Conclusion. Our findings suggest that TGF beta 1 has a protective effect on the extracellular matrix of human articular chondrocytes.
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GUNTHER M, HAUBECK HD, VANDELEUR E, et al. TRANSFORMING GROWTH-FACTOR-BETA-1 REGULATES TISSUE INHIBITOR OF METALLOPROTEINASES-1 EXPRESSION IN DIFFERENTIATED HUMAN ARTICULAR CHONDROCYTES. ARTHRITIS AND RHEUMATISM. 1994;37(3):395-405.
GUNTHER, M., HAUBECK, H. D., VANDELEUR, E., BLASER, J., BENDER, S., GUTGEMANN, I., FISCHER, D. C., et al. (1994). TRANSFORMING GROWTH-FACTOR-BETA-1 REGULATES TISSUE INHIBITOR OF METALLOPROTEINASES-1 EXPRESSION IN DIFFERENTIATED HUMAN ARTICULAR CHONDROCYTES. ARTHRITIS AND RHEUMATISM, 37(3), 395-405.
GUNTHER, M., HAUBECK, H. D., VANDELEUR, E., BLASER, J., BENDER, S., GUTGEMANN, I., FISCHER, D. C., Tschesche, H., GREILING, H., HEINRICH, P. C., et al. (1994). TRANSFORMING GROWTH-FACTOR-BETA-1 REGULATES TISSUE INHIBITOR OF METALLOPROTEINASES-1 EXPRESSION IN DIFFERENTIATED HUMAN ARTICULAR CHONDROCYTES. ARTHRITIS AND RHEUMATISM 37, 395-405.
GUNTHER, M., et al., 1994. TRANSFORMING GROWTH-FACTOR-BETA-1 REGULATES TISSUE INHIBITOR OF METALLOPROTEINASES-1 EXPRESSION IN DIFFERENTIATED HUMAN ARTICULAR CHONDROCYTES. ARTHRITIS AND RHEUMATISM, 37(3), p 395-405.
M. GUNTHER, et al., “TRANSFORMING GROWTH-FACTOR-BETA-1 REGULATES TISSUE INHIBITOR OF METALLOPROTEINASES-1 EXPRESSION IN DIFFERENTIATED HUMAN ARTICULAR CHONDROCYTES”, ARTHRITIS AND RHEUMATISM, vol. 37, 1994, pp. 395-405.
GUNTHER, M., HAUBECK, H.D., VANDELEUR, E., BLASER, J., BENDER, S., GUTGEMANN, I., FISCHER, D.C., Tschesche, H., GREILING, H., HEINRICH, P.C., GRAEVE, L.: TRANSFORMING GROWTH-FACTOR-BETA-1 REGULATES TISSUE INHIBITOR OF METALLOPROTEINASES-1 EXPRESSION IN DIFFERENTIATED HUMAN ARTICULAR CHONDROCYTES. ARTHRITIS AND RHEUMATISM. 37, 395-405 (1994).
GUNTHER, M, HAUBECK, HD, VANDELEUR, E, BLASER, J, BENDER, S, GUTGEMANN, I, FISCHER, DC, Tschesche, Harald, GREILING, H, HEINRICH, PC, and GRAEVE, L. “TRANSFORMING GROWTH-FACTOR-BETA-1 REGULATES TISSUE INHIBITOR OF METALLOPROTEINASES-1 EXPRESSION IN DIFFERENTIATED HUMAN ARTICULAR CHONDROCYTES”. ARTHRITIS AND RHEUMATISM 37.3 (1994): 395-405.
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